pLV3-CMV-Cd274-EF1a-Fluc-Puro

pLV3-CMV-Cd274-EF1a-Fluc-Puro plasmid

PVT93379

pLV3-CMV-Cd274-EF1a-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Cd274(mouse)-EF1a-Fluc-Puro
Alias: NM_021893.3;A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1;Pdl1;
Gene lenght: 873bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Cd274-EF1a-Fluc-Puro plasmid Description

pLV3-CMV-Cd274-EF1a-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Cd274-EF1a-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catgaggata tttgctggca ttatattcac agcctgctgt 2341 cacttgctac gggcgtttac tatcacggct ccaaaggact tgtacgtggt ggagtatggc 2401 agcaacgtca cgatggagtg cagattccct gtagaacggg agctggacct gcttgcgtta 2461 gtggtgtact gggaaaagga agatgagcaa gtgattcagt ttgtggcagg agaggaggac 2521 cttaagcctc agcacagcaa cttcaggggg agagcctcgc tgccaaagga ccagcttttg 2581 aagggaaatg ctgcccttca gatcacagac gtcaagctgc aggacgcagg cgtttactgc 2641 tgcataatca gctacggtgg tgcggactac aagcgaatca cgctgaaagt caatgcccca 2701 taccgcaaaa tcaaccagag aatttccgtg gatccagcca cttctgagca tgaactaata 2761 tgtcaggccg agggttatcc agaagctgag gtaatctgga caaacagtga ccaccaaccc 2821 gtgagtggga agagaagtgt caccacttcc cggacagagg ggatgcttct caatgtgacc 2881 agcagtctga gggtcaacgc cacagcgaat gatgttttct actgtacgtt ttggagatca 2941 cagccagggc aaaaccacac agcggagctg atcatcccag aactgcctgc aacacatcct 3001 ccacagaaca ggactcactg ggtgcttctg ggatccatcc tgttgttcct cattgtagtg 3061 tccacggtcc tcctcttctt gagaaaacaa gtgagaatgc tagatgtgga gaaatgtggc 3121 gttgaagata caagctcaaa aaaccgaaat gatacacaat tcgaggagac gtaaggatcc 3181 gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3241 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3301 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3361 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3421 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 3481 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 3541 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 3601 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 3661 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 3721 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gatggaagac 3781 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga 3841 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca 3901 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg 3961 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa 4021 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg 4081 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct 4141 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc 4201 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg 4261 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca 4321 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt 4381 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga 4441 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc 4501 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc 4561 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa 4621 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa 4681 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc 4741 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact 4801 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt 4861 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc 4921 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac 4981 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata 5041 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag 5101 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac 5161 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc 5221 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc 5281 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg 5341 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag 5401 aagggcggaa agatcgccgt gagatcccgc gctcagtcgt ccaattctgc cgtggacggc 5461 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 5521 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 5581 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5641 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5701 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5761 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5821 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5881 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5941 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 6001 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 6061 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 6121 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 6181 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6241 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6301 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6361 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6421 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 6541 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6601 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6661 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 6721 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 6781 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 6841 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6901 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6961 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7021 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 7081 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 7141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 7261 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 7321 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 7381 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 7441 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7501 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7561 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 7621 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 8281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 9061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 9601 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 9661 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 9721 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 9781 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 9841 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 9901 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 9961 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 10021 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.