pCAMBIA1300-35S-mCherry-Golgi

pCAMBIA1300-35S-mCherry-Golgi plasmid

PVT93206

pCAMBIA1300-35S-mCherry-Golgi plasmid Information

Plasmid name: pCAMBIA1300-35S-mCherry-Golgi
Alias: -
Gene lenght:
Host: Plant
Use(s): Protein Expression,subcellular localization
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Red
Promoter: 35S
Replicon: pVS1
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: 35S
Reverse primer:
Copy number: Low
Induction:

pCAMBIA1300-35S-mCherry-Golgi plasmid Description

pCAMBIA1300-35S-mCherry-Golgi is a plasmid suitable for protein expression in plants, subcellular localization.

pCAMBIA1300-35S-mCherry-Golgi plasmid Sequence

1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta 61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa 121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca 181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc 241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc 301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg 361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct 421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct 481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg 541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct 601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca 661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa 721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac 781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga 841 gaacacgggg gacgagctcg gtacccggga tccTCTAGAa tggtgagcaa gggcgaggag 901 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 961 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 1021 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 1081 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 1141 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 1201 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg 1261 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 1321 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 1381 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 1441 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc 1501 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 1561 accggcggca tggacgagct gtacaaggtc gacgtccaag actataacca tgataaagtt 1621 gtttcccgag gatcgaaaaa gtatgtggaa gatcttgaaa gtggattcag ctcggatgtc 1681 gaatcaaaat acaagaaaat ttacgaagat gacatcaacc cttttgcagc attctcgaaa 1741 aaggaaagag agcaacggat caaagatttg ggaatcagag atcggattac gctaagcagt 1801 gggcggttcc ttctaggaaa caaatacgca aggacatttg ctttcttcta cacaatagga 1861 ttgcacgtcc ttgtcttcac ttgtctctac cgtatgtctg cttacagtta tctcagccat 1921 ggagcagagg agactctaat gacagaagca accacaaacc ttcctcacgg tctttaactg 1981 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt 2041 cattgcgcac acaccagaat cctactgagt tcgagtatta tggcattggg aaacatgttt 2101 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 2161 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 2221 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaaatataa 2281 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 2341 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac 2401 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg 2461 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta 2521 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt 2581 ttaatgcatt ttatgacttg ccaattgatt gacaacatgc atcaagcttg gcactggccg 2641 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 2701 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 2761 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 2821 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 2881 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 2941 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 3001 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 3061 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 3121 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 3181 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 3241 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 3301 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 3361 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 3421 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 3481 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 3541 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 3601 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 3661 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 3721 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 3781 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 3841 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 3901 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 3961 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 4021 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 4081 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 4141 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 4201 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 4261 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 4321 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 4381 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 4441 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 4501 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 4561 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 4621 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 4681 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 4741 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 4801 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 4861 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 4921 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 4981 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 5041 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 5101 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 5161 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 5221 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 5281 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 5341 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 5401 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 5461 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 5521 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 5581 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 5641 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 5701 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 5761 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 5821 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 5881 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 5941 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 6001 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 6061 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 6121 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 6181 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 6241 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 6301 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 6361 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 6421 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 6481 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 6541 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 6601 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 6661 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 6721 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 6781 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 6841 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 6901 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 6961 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 7021 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 7081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 7381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 7441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 7501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 7561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 7621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 7681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 7741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 7801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 7861 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 7921 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 7981 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 8041 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 8101 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 8161 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 8221 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 8281 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 8341 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 8401 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 8461 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 8521 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 8581 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 8641 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 8701 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 8761 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 8821 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 8881 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 8941 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 9001 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 9061 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 9121 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 9181 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 9241 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 9301 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 9361 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga 9421 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc 9481 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc 9541 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac 9601 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa 9661 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc 9721 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt 9781 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta 9841 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga 9901 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac 9961 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata 10021 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc 10081 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa 10141 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga 10201 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg 10261 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat 10321 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc 10381 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga 10441 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag 10501 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga 10561 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc 10621 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 10681 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 10741 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 10801 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 10861 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 10921 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt 10981 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 11041 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 11101 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 11161 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 11221 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct 11281 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc 11341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 11401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 11461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 11521 ccatgattac g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.