pTac-GST-NAT8 plasmid

pTac-GST-NAT8 plasmid

PVT93190

pTac-GST-NAT8 plasmid Information

Plasmid name: pTac-GST-NAT8(human)
Alias: NM_003960.4;ATase2; CCNAT; CML1; GLA; Hcml1; TSC501;TSC510;
Gene lenght: 684bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pGEX-5'
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-NAT8 plasmid Description

pTac-GST-NAT8 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-NAT8 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggc tccttgtcac atccgcaaat 961 accaggagag cgaccgccag tgggttgtgg gcttgctctc ccgggggatg gccgagcatg 1021 ccccagccac cttccggcaa ttgctgaagc tgcctcgaac cctcatactc ttacttgggg 1081 ggcccctcgc cctactcctg gtctctggat cctggcttct agccctcgtg ttcagcatca 1141 gcctcttccc tgccctgtgg ttccttgcca aaaaaccctg gacggagtat gtggacatga 1201 cattgtgcac agacatgtct gacattacca aatcctacct gagtgagcgt ggctcctgct 1261 tctgggtggc tgagtctgaa gagaaggtgg tgggcatggt aggagctctg cctgttgatg 1321 atcccacctt gagggagaag cggttgcagc tgtttcatct ctttgtggac agtgagcacc 1381 gtcgtcaggg gatagcaaaa gccctggtca ggactgtcct ccagtttgcc cgggaccagg 1441 gctacagtga agttatcctg gacaccggca ccatccagct ctctgctatg gccctctacc 1501 agagcatggg cttcaagaag acgggccagt ccttcttctg tgtgtgggcc aggctagtgg 1561 ctcttcatac agttcatttc atctaccacc tcccttcttc taaggtaggg agtctgtgac 1621 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg 1681 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 1741 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 1801 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga 1861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 1921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 1981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 2041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 2101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 2161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 2221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 2281 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 2341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 2401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 2461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 2521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 2581 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 2641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 2701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 2761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 2821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 2881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 2941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 3001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 3061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 3121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 3181 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 3241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 3301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 3361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 3421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 3481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 3541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 3601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 3661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 3721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 3781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 3841 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa 3901 attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag 3961 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 4021 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 4081 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 4141 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 4201 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 4261 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 4321 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 4381 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 4441 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 4501 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 4561 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 4621 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 4681 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 4741 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 4801 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 4861 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 4921 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 4981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5041 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 5101 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 5161 ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt 5221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 5281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 5341 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc 5401 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg 5461 gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa 5521 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga 5581 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgttg gaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.