pCMV-LOC100505715-Neo plasmid

pCMV-LOC100505715-Neo plasmid

PVT93144

pCMV-LOC100505715-Neo plasmid Information

Plasmid name: pCMV-LOC100505715(human)-Neo
Alias: NR_038334.1
Gene lenght: 2193bp
Host: Mammalian cells
Use(s): Transposition regulation
Fragment type: ncRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-LOC100505715-Neo plasmid Description

pCMV-LOC100505715-Neo is a plasmid suitable for transcriptional regulation in mammalian cells.

pCMV-LOC100505715-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGAGCTGCTG TAGCCCCTAC GTGGTGAGTA GATCGTCTCC ACAGAAATCA GCGGAGGCTT 961 CCACAGTCCC TGGTTGGAAT GGAACCGGCG GTGAGGATGA GAGGCGCTGA AGACCAGAAG 1021 ACCCAGGGCG TGAACGGGGC GTGTCATCCC TGGAGTTTCA GTAGAATTTC AGGCCTGTCT 1081 GCTTGAGCAG CAGAGGAGGA GTTTCTGTGT GCCTGTGAGA GACTGTGTGT GTGTGTGTGT 1141 GTGTGTGTGT GTGTGAGATG AATGAACAGG GTTGTGTACG TGTGTGTGCG TTAGACAAAT 1201 GAACAGTGTT GTGTACCTGT GTGAATTGTC ACTGTGACTC CAGGCTCTAG GCTCTGCAGA 1261 TTCCTCAGCC CTATCGCCTC ACAGACACTT GGGATGTGCA GCAGGTAAAA GAGAGAAGCC 1321 AGATTCATGG AGGCGTGAAG GATCCCAGCA AGAAAACACA GACTTGATCT TGAGACGAGA 1381 TTTTTGAAAT TGGTTGCGGC CTCAAGGCAG GCTCTGAGAA GACTCGATAA AGAGTTCTCA 1441 GGAATAGGCA TCCCTCCTGG ATATTTCCCC CTTGGAAGCG CCGAGGAGCC AGCTCTATGT 1501 TTGTAACCAT AGCATTTCTA AAGCTCCAGC CCCAGGAACC TGCTGTGAAT TAGAGACAGA 1561 TTCCACCCTA GGCATCACTG ATCTTGGCCT CTCTTGTATT GGGAAGGTCA ACACTGTTCC 1621 AGTTTCTCTC CTACTCCCAT CTGGTTGTGC TCGGTGAAGT AGGTGAGCAG TGCTACCCTT 1681 CTGTTAAAAA AAAAAAAAAA AAAAAGATCC TGAAAGAGGA AAGAATTTTT GCTACACATC 1741 TGAAAGCTAA AGTCCAGTTG AACTCCAGAG GAGAACACAG CCCAGGTAAC CCCTCGTTTG 1801 CAGCCTGTGA GACCAGCCTG AGGACAGCAC CCAGCTAAAC TCCAGGTCCA TGGAAACTGT 1861 GAGATTTTAT ATATTTATGT TGTTTTAAGC TAAATTCGTG GTAATTGGTT ATGGTAGCAA 1921 CTGAAAATTA ATGCAGTAAG AAAGTCTAAG GAGATCGGAA GAGACTAAAG AGAAATAACA 1981 GCTAAATGCA ACGTAGATTA ATGTGTGGGA TCCTGGAAAA GAAAAAGGAC ATTAATGGGA 2041 AAACATATTT GAATAAGATG TGCAAATTTT TCGTATTGTA CCAATGTTAA TTTCCTGGTT 2101 TTGATAATTA TCCTTTGGTT ATGTAAGATA AACGCTAGAA TGAAGGGTAT ATGAGAACCC 2161 TCTGTACTAC TCTTGCAACT TTTCTCTAAG TCTAAAATAA GTGGAAATTA AAAGTTAAAA 2221 CAAAAAAAAA GGAAAATTTT ATCTATCCCA AGACCACAAA GTTATTTTTC TGTTTCTTCC 2281 TCCAGAATCT TTATTGTTTG TTTTCACACT TAGACCTATG ATCCATCTTG AATTTATATC 2341 TGTGTATGTA GTGGGATGAA GTTCAAAATC ATTTTTTTCA TACAGATATC CAAGTGCACT 2401 GGCACCGTTT GTTAAAAACA CTGGCCTTCT CTTAAGCTGC AGTCAGACTT TTACTGTAAA 2461 GCATGGGCCC ATATATGTGC AGGAGTCTTT CTACTTTTCC GTTGGTCCTC TTTGTCAATC 2521 TTTGTGCCAG CACCTCCATT TTAATTCCTG TAGCTTTATA TTATATCTTG ATAATCTGGT 2581 AATATAAGTT GAAGAACAAC TTTGTTCTTC AAAATCACTT TGTCTATTCC AGGTTCTTTG 2641 CATTGCTCTA TAATTTTAGA ATGAGTTAAT TAATTTTCTC AAAACAAACA AAACGCCCTG 2701 CTGACATTTT GTTGGCAGTA CATTGAATCA GTAGATAAAT TTAGAGAGAA TTATCTCAAA 2761 ATTGAGTCTT ACAGTTCATG AACATGGAAT ACATTTCCAT TTATTGAATT CATCTTTAAT 2821 TTCTTTCCTT AAGGTTTTGT TGTTTCCTGT CTAAAGGTCT TGCACATCTG TTAAATTTAT 2881 TCCTGTTTTA CATTTTTTAT GTTTTTACAA ACTGTTAAGG TTTTAAAAAT TTTATTTTTC 2941 AGTAGCTTTG CTTTAATATA ATTGTTTTAT ACTTTTACCC TTTATTCAGC AATAAATTAT 3001 TTAATTTAAA GCTTGTGGAA TTAAAAAATA TTTTTATGTA CATAATCATG TAATCTACAC 3061 TATCTGCAAA TAAAGATCAT TTTACTTCCT TCCACTCGAG TCTAGAGGGC CCGTTTAAAC 3121 CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC 3181 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA 3241 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA 3301 CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT 3361 GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 3421 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 3481 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 3541 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 3601 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 3661 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 3721 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 3781 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 3841 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 3901 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 3961 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 4021 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 4081 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 4141 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 4201 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 4261 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 4321 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 4381 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 4441 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 4501 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 4561 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 4621 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 4681 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 4741 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 4801 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 4861 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 4921 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 4981 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 5041 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 5101 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 5161 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 5221 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 5281 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 5341 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 5401 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 5461 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 5521 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 5581 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 5641 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 5701 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 5761 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 5821 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 5881 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 5941 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 6001 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 6061 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 6121 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 6181 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 6241 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 6301 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 6361 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 6421 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 6481 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 6541 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 6601 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 6661 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 6721 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 6781 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 6841 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 6901 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 6961 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 7021 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 7081 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 7141 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 7201 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 7261 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 7321 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 7381 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 7441 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 7501 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA 7561 TCCCCTATGG T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.