pCMV-FLAG-Hif1a-HA-Neo plasmid

pCMV-FLAG-Hif1a-HA-Neo plasmid

PVT93088

pCMV-FLAG-Hif1a-HA-Neo plasmid Information

Plasmid name: pCMV-FLAG-Hif1a(mouse)-HA-Neo
Alias: NM_010431.3;bHLHe78; HIF-1-alpha; HIF1-alpha;HIF1alpha; MOP1
Gene lenght: 2508bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FLAG-Hif1a-HA-Neo plasmid Description

pCMV-FLAG-Hif1a-HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FLAG-Hif1a-HA-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 gccaccATGG ATTACAAGGA TGACGACGAT AAGACTAAGC TTatggaggg cgccggcggc 961 gagaacgaga agaaaaagat gagttctgaa cgtcgaaaag aaaagtctag agatgcagca 1021 agatctcggc gaagcaaaga gtctgaagtt ttttatgagc ttgctcatca gttgccactt 1081 ccccacaatg tgagctcaca tcttgataaa gcttctgtta tgaggctcac catcagttat 1141 ttacgtgtga gaaaacttct ggatgccggt ggtctagaca gtgaagatga gatgaaggca 1201 cagatggact gtttttatct gaaagcccta gatggctttg tgatggtgct aacagatgac 1261 ggcgacatgg tttacatttc tgataacgtg aacaaataca tggggttaac tcagtttgaa 1321 ctaactggac acagtgtgtt tgattttact catccatgtg accatgagga aatgagagaa 1381 atgcttacac acagaaatgg cccagtgaga aaagggaaag aactaaacac acagcggagc 1441 ttttttctca gaatgaagtg caccctaaca agccggggga ggacgatgaa catcaagtca 1501 gcaacgtgga aggtgcttca ctgcacgggc catattcatg tctatgatac caacagtaac 1561 caacctcagt gtgggtacaa gaaaccaccc atgacgtgct tggtgctgat ttgtgaaccc 1621 attcctcatc cgtcaaatat tgaaattcct ttagatagca agacatttct cagtcgacac 1681 agcctcgata tgaaattttc ttactgtgat gaaagaatta ctgagttgat gggttatgag 1741 ccggaagaac ttttgggccg ctcaatttat gaatattatc atgctttgga ttctgatcat 1801 ctgaccaaaa ctcaccatga tatgtttact aaaggacaag tcaccacagg acagtacagg 1861 atgcttgcca aaagaggtgg atatgtctgg gttgaaactc aagcaactgt catatataat 1921 acgaagaact cccagccaca gtgcattgtg tgtgtgaatt atgttgtaag tggtattatt 1981 cagcacgact tgattttctc ccttcaacaa acagaatctg tgctcaaacc agttgaatct 2041 tcagatatga agatgactca gctgttcacc aaagttgaat cagaggatac aagctgcctt 2101 tttgataagc ttaagaagga gcctgatgct ctcactctgc tggctccagc tgccggcgac 2161 accatcatct ctctggattt tggcagcgat gacacagaaa ctgaagatca acaacttgaa 2221 gatgttccat tatataatga tgtaatgttt ccctcttcta atgaaaaatt aaatataaac 2281 ctggcaatgt ctcctttacc ttcatcggaa actccaaagc cacttcgaag tagtgctgat 2341 cctgcactga atcaagaggt tgcattaaaa ttagaatcaa gtccagagtc actgggactt 2401 tcttttacca tgccccagat tcaagatcag ccagcaagtc cttctgatgg aagcactaga 2461 caaagttcac ctgagagact tcttcaggaa aacgtaaaca ctcctaactt ttcccagcct 2521 aacagtccca gtgaatattg ctttgatgtg gatagcgata tggtcaatgt attcaagttg 2581 gaactggtgg aaaaactgtt tgctgaagac acagaggcaa agaatccatt ttcaactcag 2641 gacactgatt tagatttgga gatgctggct ccctatatcc caatggatga tgatttccag 2701 ttacgttcct ttgatcagtt gtcaccatta gagagcaatt ctccaagccc tccaagtatg 2761 agcacagtta ctgggttcca gcagacccag ttacagaaac ctaccatcac tgccactgcc 2821 accacaactg ccaccactga tgaatcaaaa acagagacga aggacaataa agaagatatt 2881 aaaatactga ttgcatctcc atcttctacc caagtacctc aagaaacgac cactgctaag 2941 gcatcagcat acagtggcac tcacagtcgg acagcctcac cagacagagc aggaaagaga 3001 gtcatagaac agacagacaa agctcatcca aggagcctta acctgtctgc cactttgaat 3061 caaagaaata ctgttcctga ggaagaatta aacccaaaga caatagcttc gcagaatgct 3121 cagaggaagc gaaaaatgga acatgatggc tccctttttc aagcagcagg aattggaaca 3181 ttattgcagc aaccaggtga ctgtgcacct actatgtcac tttcctggaa acgagtgaaa 3241 ggattcatat ctagtgaaca gaatggaacg gagcaaaaga ctattatttt aataccctcc 3301 gatttagcat gcagactgct ggggcagtca atggatgaga gtggattacc acagctgacc 3361 agttacgatt gtgaagttaa tgctcccata caaggcagca gaaacctact gcagggtgaa 3421 gaattactca gagctttgga tcaagttaac CTCGAGTACC CATACGACGT ACCTGATTAT 3481 GCATAGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGTTT 3541 AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT 3601 CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG 3661 AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC 3721 AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT 3781 CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT 3841 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 3901 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 3961 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 4021 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 4081 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 4141 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 4201 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 4261 AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 4321 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC 4381 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 4441 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 4501 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA 4561 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG 4621 TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA 4681 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG 4741 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG 4801 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC 4861 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT 4921 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC 4981 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA 5041 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC 5101 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG 5161 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT 5221 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC 5281 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC 5341 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA 5401 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT 5461 CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA 5521 GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC 5581 GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC 5641 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 5701 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT 5761 CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT 5821 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG 5881 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG 5941 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 6001 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 6061 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 6121 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 6181 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 6241 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 6301 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 6361 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 6421 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 6481 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 6541 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 6601 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 6661 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 6721 GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 6781 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 6841 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 6901 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 6961 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 7021 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 7081 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 7141 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 7201 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 7261 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 7321 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 7381 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 7441 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 7501 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 7561 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 7621 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 7681 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 7741 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 7801 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 7861 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 7921 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.