pSin-EF2-RTN3-EGFP-IRES-Blast

pSin-EF2-RTN3-EGFP-IRES-Blast plasmid

PVT93045

pSin-EF2-RTN3-EGFP-IRES-Blast plasmid Information

Plasmid name: pSin-EF2-RTN3(human)-EGFP-IRES-Blast
Alias: NM_001265589.1;ASYIP; HAP; NSPL2; NSPLII; RTN3-A1;
Gene lenght: 3096bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pSin-EF2-RTN3-EGFP-IRES-Blast plasmid Description

pSin-EF2-RTN3-EGFP-IRES-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pSin-EF2-RTN3-EGFP-IRES-Blast plasmid Sequence

1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga 121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca 181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca 2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg 2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga 2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 2701 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 2761 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 2821 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 2881 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt 2941 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 3001 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 3061 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 3121 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 3181 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 3241 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 3301 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 3361 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 3421 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 3481 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 3541 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattgggatc 3601 cGCCACCatg gcggagccgt cggcggccac tcagtcccat tccatctcct cgtcgtcctt 3661 cggagccgag ccgtccgcgc ccggcggcgg cgggagccca ggagcctgcc ccgccctggg 3721 gacgaagagc tgcagctcct cctgtgcgga ttcctttgtt tcttcctctt cctctcagcc 3781 tgtatctcta ttttcgacct cacaagaggg attgagctct ctttgctctg atgagccatc 3841 ttcagaaatt atgacttctt cctttctttc atcttctgaa atacataaca ctggccttac 3901 aatactacat ggagaaaaaa gccatgtgtt agggagccag cctattttag ccaaagaagg 3961 aaaagaccac ttggatcttc tagatatgaa aaagatggaa aagcctcagg ggaccagcaa 4021 caacgtatca gactcttcag tttctcttgc agcaggagtt cattgtgacc gtccttctat 4081 tccagccagt ttcccagagc atcctgcttt tctctcaaag aaaattggtc aagtggaaga 4141 gcaaatagat aaagagacca agaacccaaa tggggtatca agtagggagg ctaaaactgc 4201 attggatgct gatgacagat tcactttgct gacagcccag aaaccaccta ctgagtactc 4261 taaggtagaa ggcatttata catattcttt gtctccatcc aaagtttcag gagatgatgt 4321 tattgaaaag gattcccctg aatcaccatt tgaagtaatt attgacaaag cagcatttga 4381 caaagaattt aaagactcat ataaggagag cacagatgat tttggtagct ggtctgtgca 4441 cactgataaa gaatcatccg aagacatttc agagactaat gacaagcttt ttccactgag 4501 aaataaagag gcaggacgtt acccaatgtc tgcattgctc agtaggcagt tttcacacac 4561 aaatgcagca ctggaagagg tgtccagatg cgtgaatgat atgcataact ttactaacga 4621 aatactgact tgggatctgg ttccccaagt gaaacaacag accgataaat cttctgactg 4681 catcacaaaa actacaggac ttgacatgag tgaatataat tcagaaattc cagttgtaaa 4741 tcttaaaact agcactcatc agaaaactcc tgtatgttct attgatggga gcactcccat 4801 cactaaatca acaggtgatt gggcagaagc atctctccag caagaaaatg ctattactgg 4861 aaaacctgta cctgactctt tgaattccac aaaagaattc agtatcaaag gtgtgcaagg 4921 caatatgcag aaacaggatg acacacttgc agaattacct ggatctccac ctgagaaatg 4981 tgactctttg ggttctggag tggccacagt gaaagtggtt ttacctgatg accacctgaa 5041 agatgaaatg gactggcaga gctctgcatt gggagaaatc acagaagctg atagttctgg 5101 tgagtctgat gacacagtaa tagaggacat cacagcagat acatcatttg aaaataacaa 5161 aattcaggct gaaaaacctg tttccattcc aagtgctgtt gtaaaaacag gtgaaagaga 5221 aatcaaagag attcccagtt gtgagagaga agaaaaaaca tctaaaaact ttgaagaatt 5281 ggtcagtgac tctgagctgc atcaagatca gcctgatatt cttggaagga gtccagctag 5341 tgaggcagca tgttcaaaag tacccgatac gaatgtctcc ttagaagatg tgagtgaagt 5401 tgctcctgaa aagcctatta ctactgagaa ccccaaactt ccttcaacag tgtctccaaa 5461 tgtttttaat gagacagaat tctcattaaa tgtgacaaca tctgcctatt tggagtcatt 5521 acatgggaaa aatgttaaac atatagatga ttcctcccca gaggacctga tagcagcctt 5581 tacagaaacc agagataaag gaatagtaga tagtgaaaga aatgctttta aagcaatatc 5641 agagaagatg acagacttta aaacaactcc tcctgtagaa gtcttacatg aaaatgagtc 5701 cggtggttct gaaattaaag acattggaag caaatacagt gaacaaagca aagaaacaaa 5761 tggaagtgag cctctaggtg ttttccctac ccaaggtact ccagtagcat ctcttgactt 5821 agaacaagaa cagctcacaa ttaaggctct taaagaatta ggtgaaagac aggttgagaa 5881 gtcaacttct gcacagcgtg acgcagaatt gccttctgaa gaagtactga agcaaacttt 5941 cacatttgct ccagaatctt ggccacagag atcatatgac atcctagaac gtaatgtcaa 6001 gaatggatct gatcttggga tttcccagaa gcccatcact atcagagaaa ctactagggt 6061 agatgctgtt tccagcctta gcaagactga attggtaaaa aagcatgtcc tagcaagact 6121 tctgacagac ttctcagtgc acgatctgat tttctggaga gatgtgaaga agactgggtt 6181 tgtctttggc accacgctga tcatgctgct ttccctggca gctttcagtg tcatcagtgt 6241 ggtttcttac ctcatcctgg ctcttctctc tgtcaccatc agcttcagga tctacaagtc 6301 cgtcatccaa gctgtacaga agtcagaaga aggccatcca ttcaaagcct acctggacgt 6361 agacattact ctgtcctcag aagctttcca taattacatg aatgctgcca tggtgcacat 6421 caacagggcc ctgaaactca ttattcgtct ctttctggta gaagatctgg ttgactcctt 6481 gaagctggct gtcttcatgt ggctgatgac ctatgttggt gctgttttta acggaatcac 6541 ccttctaatt cttgctgaac tgctcatttt cagtgtcccg attgtctatg agaagtacaa 6601 gacccagatt gatcactatg ttggcatcgc ccgagatcag accaagtcaa ttgttgaaaa 6661 gatccaagca aaactccctg gaatcgccaa aaaaaaggca gaaGGCGGTG GAGGAAGCgg 6721 cggaggtgga tctatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 6781 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 6841 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 6901 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 6961 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 7021 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 7081 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 7141 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 7201 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 7261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 7321 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 7381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 7441 gctgtacaag taggaattct tcgaacatat gaagcttgct agcactagtg atccgcatgc 7501 atctagggcg gccaattccg cccctctccc ccccccccct ctccctcccc cccccctaac 7561 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 7621 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 7681 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 7741 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 7801 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 7861 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 7921 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 7981 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg 8041 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 8101 cgatgataag cttgccacaa cccacaagga gacgaccttc catggccaag cctttgtctc 8161 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg 8221 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca 8281 atgtatatca ttttactggg ggaccttgcg cagaactcgt ggtgctgggc actgctgctg 8341 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct 8401 tgagcccctg cggacggtgc cgacaggttc ttctcgatct gcatcctggg atcaaagcca 8461 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg 8521 gttatgtgtg ggagggctaa taataggcgg ccgctcgaga cctagaaaaa catggagcaa 8581 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 8641 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 8701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 8761 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 8821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 8881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 8941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 9001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattata 9061 atagatttag caggaattga actaggagtg gagcacacag gcaaagttct agagctcgct 9121 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 9181 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 9241 catcgcattg tctgagtagg tgtcattcta ttctgggggg tgggggtggg gcaggacagc 9301 aaggggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 9361 ttctgaggcg gaaagaacca gctgggggcg cgcccctcga ggccgccatg gtcatagctg 9421 tttgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 9481 ctaaatacat tcaaatatgt atccgctcac gagacaataa ccctgataaa tgcttcaata 9541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 9601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 9661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 9721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 9781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 9841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 9901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 9961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 10021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 10081 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 10141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 10201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 10261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 10321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 10381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 10441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 10501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 10561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 10621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 10681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 10741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 10801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 10861 gttggactca agacgatagt taccggataa gcgcagcggt cgggctgaac ggggggttcg 10921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 10981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 11041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 11101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 11161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttctg gccttttgca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.