pCMV-OGDH-3×FLAG-Neo plasmid

pCMV-OGDH-3×FLAG-Neo plasmid

PVT92792

pCMV-OGDH-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-OGDH(human)-3×FLAG-Neo
Alias: NM_002541.4;AKGDH; E1k; E1o; KGD1; OGDC; OGDH-E1;OGDH2; OGDHD
Gene lenght: 3069bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-OGDH-3×FLAG-Neo plasmid Description

pCMV-OGDH-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-OGDH-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatgtttc atttaaggac ttgtgctgct aagttgaggc cattgacggc ttcccagact 1021 gttaagacat tttcacaaaa cagaccagca gcagctagga catttcaaca gattcggtgc 1081 tattctgcac ctgttgctgc tgagcccttt ctcagtggga ctagttcgaa ctatgtggag 1141 gagatgtact gtgcttggct ggaaaacccc aaaagtgtac ataagtcatg ggacattttt 1201 tttcgcaaca cgaatgccgg agccccaccg ggcactgcct accagagtcc ccttcccctg 1261 agccgaggct ccctggctgc tgtggcccat gcacagtccc tggtagaagc acagcccaac 1321 gtggacaagc tcgtggagga ccacctggca gtgcagtcgc tcatcagggc atatcagata 1381 cgagggcacc atgtagcaca gctggacccc ctggggattt tggatgctga tctggactcc 1441 tccgtgcccg ctgacattat ctcatccaca gacaaacttg ggttctatgg cctggatgag 1501 tctgacctcg acaaggtctt ccacttgccc accaccactt tcatcggggg acaggaatca 1561 gcacttcctc tgcgggagat catccgtcgg ctggagatgg cctactgcca gcatattggg 1621 gtggagttca tgttcatcaa tgacctggag cagtgccagt ggatccggca gaagtttgag 1681 acccctggga tcatgcagtt cacaaatgag gagaaacgga ccctgctggc caggcttgtg 1741 cggtccacca ggtttgagga gttcctacag cggaagtggt cctctgagaa gcgctttggt 1801 ctagaaggct gcgaggtact gatccctgcc ctcaagacca tcattgacaa gtctagtgag 1861 aatggcgtgg actacgtgat catgggcatg ccacacagag ggcggctgaa cgtgcttgca 1921 aatgtcatca ggaaggagct ggaacagatc ttctgtcaat tcgattcaaa gctggaggca 1981 gctgatgagg gctccggaga tgtgaagtac cacctgggca tgtatcaccg caggatcaat 2041 cgtgtcaccg acaggaacat taccttgtcc ttggtggcca acccttccca ccttgaggcc 2101 gctgaccccg tggtgatggg caagaccaaa gccgaacagt tttactgtgg cgacactgaa 2161 gggaaaaagg tcatgtccat cctgttgcat ggggatgctg catttgctgg ccagggcatt 2221 gtgtacgaga ccttccacct cagcgacctg ccatcctaca caactcatgg caccgtgcac 2281 gtggtcgtca acaaccagat cggcttcacc accgaccctc ggatggcccg ctcctccccc 2341 taccccactg acgtggcccg agtggtgaat gcccccattt tccacgtgaa ctcagatgac 2401 cccgaggctg tcatgtacgt gtgcaaagtg gcggccgagt ggaggagcac cttccacaag 2461 gacgtggttg tcgatttggt gtgttaccgg cgcaacggcc acaacgagat ggatgagccc 2521 atgttcacgc agccgctcat gtacaagcag atccgcaagc agaagcctgt gttacagaag 2581 tacgctgagc tgctggtgtc gcagggtgtg gtcaaccagc ctgagtatga ggaggaaatt 2641 tccaagtatg ataagatctg tgaggaagct tttgccagat ctaaagatga gaagatcttg 2701 cacattaagc actggctgga ctctccctgg cctggcttct tcaccctgga cgggcagccc 2761 aggagcatgt cctgcccctc cacgggtctg acggaggata ttctgacaca catcgggaat 2821 gtggctagtt ctgtgcctgt ggaaaacttt actattcatg gagggctgag ccggatcttg 2881 aagactcgtg gggaaatggt gaagaaccgg actgtggact gggctctagc ggagtacatg 2941 gcgtttggct cgctcctgaa ggagggcatc cacattcggc tgagcggcca ggacgtggag 3001 cggggcacat tcagccaccg ccaccatgtg ctccatgacc agaatgtgga caagagaacc 3061 tgcatcccca tgaaccatct ctggcccaat caggccccct atactgtgtg caacagctca 3121 ctgtctgagt acggcgtgct gggctttgag ctgggcttcg ccatggccag tcctaatgcc 3181 ctggtcctct gggaagccca atttggtgac ttccacaaca cggcccagtg tatcatcgac 3241 cagttcatct gcccgggaca agccaagtgg gtgcggcaga atggcatcgt gttgctgctg 3301 ccccatggca tggagggcat gggtccagaa cattcctccg cccgcccaga gcggttcttg 3361 cagatgtgca acgatgaccc agatgtcctg ccagacctta aagaagccaa cttcgacatc 3421 aatcagctat atgactgcaa ttgggttgtt gtcaactgct ccactcctgg caacttcttc 3481 cacgtgctac gacgccagat cctgctgcca ttccggaagc cgttaattat cttcaccccc 3541 aaatccctgt tgcgccaccc cgaggccaga tccagctttg atgagatgct tccaggaacc 3601 cacttccagc gggtgatccc agaagatggc cctgcagctc agaacccaga aaatgtcaaa 3661 aggcttctct tctgcaccgg caaagtgtat tatgacctca cccgggagcg caaagcacgc 3721 gacatggtgg ggcaggtggc catcacaagg attgagcagc tgtcgccatt cccctttgac 3781 ctcctgctga aggaggtgca gaagtacccc aatgctgagc tggcctggtg ccaggaggag 3841 cacaagaacc aaggctacta tgactacgtg aagccaagac ttcggaccac catcagccgc 3901 gccaagcccg tctggtatgc cggccgggac ccagcggctg ctccagccac cggcaacaag 3961 aagacccacc tgacggagct gcagcgcctc ctggacacgg ccttcgacct ggacgtcttc 4021 aagaacttct cgCTCGAGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 4081 GACTACAAGG ATGACGATGA CAAGTAGTGA GGGCCCGTTT AAACCCGCTG ATCAGCCTCG 4141 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC 4201 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT 4261 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT 4321 TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA 4381 AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG 4441 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 4501 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 4561 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 4621 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 4681 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 4741 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 4801 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC 4861 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4921 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 4981 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 5041 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 5101 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 5161 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 5221 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 5281 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 5341 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 5401 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 5461 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 5521 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 5581 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 5641 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 5701 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT 5761 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 5821 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC 5881 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 5941 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 6001 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT 6061 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 6121 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 6181 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 6241 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 6301 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 6361 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6421 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6481 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6541 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6601 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 6661 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 6721 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 6781 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 6841 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 6901 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 6961 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 7021 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 7081 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 7141 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 7201 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 7261 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7321 TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 7381 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 7441 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 7501 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 7561 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 7621 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 7681 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 7741 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 7801 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 7861 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 7921 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 7981 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 8041 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 8101 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 8161 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 8221 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 8281 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 8341 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 8401 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 8461 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 8521 CCGAAAAGTG CCACCTGACG TC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.