pU6-SF3B5-sgRNA1-Cas9-Neo plas

pU6-SF3B5-sgRNA1-Cas9-Neo plasmid

PVT92710

pU6-SF3B5-sgRNA1-Cas9-Neo plasmid Information

Plasmid name: pU6-SF3B5(human)-sgRNA1-Cas9-Neo
Alias: GTGGCCCGTGCCGATGTACT
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-SF3B5-sgRNA1-Cas9-Neo plasmid Description

pU6-SF3B5-sgRNA1-Cas9-Neo is a plasmid suitable for gene editing in mammalian cells.

pU6-SF3B5-sgRNA1-Cas9-Neo plasmid Sequence

1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 241 CGAAACACCg gGTGGCCCGT GCCGATGTAC Tgttttagag ctaGAAAtag caagttaaaa 301 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcTTT TTTgttttag 361 agctagaaat agcaagttaa aataaggcta gtccgtTTTT agcgcgtgcg ccaattctgc 421 agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga 481 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga 541 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 601 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattGtgcc 661 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 721 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 781 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 841 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 901 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 961 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg 1021 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 1081 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 1141 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa 1201 ttacctggag cacctgcctg aaatcacttt ttttcaggtt GGaccggtgc caccATGGAC 1261 TATAAGGACC ACGACGGAGA CTACAAGGAT CATGATATTG ATTACAAAGA CGATGACGAT 1321 AAGATGGCCC CAAAGAAGAA GCGGAAGGTC GGTATCCACG GAGTCCCAGC AGCCGACAAG 1381 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC 1441 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC 1501 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG 1561 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA 1621 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG 1681 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG 1741 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG 1801 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG 1861 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG 1921 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC 1981 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA 2041 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC 2101 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA 2161 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC 2221 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC 2281 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG 2341 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG 2401 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT 2461 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG 2521 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTGCG GAAGCAGCGG 2581 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG 2641 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC 2701 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC 2761 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC 2821 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC 2881 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG 2941 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG 3001 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG 3061 CTGAAAGAGG ACTACTTCAA GAAAATCGAG TGCTTCGACT CCGTGGAAAT CTCCGGCGTG 3121 GAAGATCGGT TCAACGCCTC CCTGGGCACA TACCACGATC TGCTGAAAAT TATCAAGGAC 3181 AAGGACTTCC TGGACAATGA GGAAAACGAG GACATTCTGG AAGATATCGT GCTGACCCTG 3241 ACACTGTTTG AGGACAGAGA GATGATCGAG GAACGGCTGA AAACCTATGC CCACCTGTTC 3301 GACGACAAAG TGATGAAGCA GCTGAAGCGG CGGAGATACA CCGGCTGGGG CAGGCTGAGC 3361 CGGAAGCTGA TCAACGGCAT CCGGGACAAG CAGTCCGGCA AGACAATCCT GGATTTCCTG 3421 AAGTCCGACG GCTTCGCCAA CAGAAACTTC ATGCAGCTGA TCCACGACGA CAGCCTGACC 3481 TTTAAAGAGG ACATCCAGAA AGCCCAGGTG TCCGGCCAGG GCGATAGCCT GCACGAGCAC 3541 ATTGCCAATC TGGCCGGCAG CCCCGCCATT AAGAAGGGCA TCCTGCAGAC AGTGAAGGTG 3601 GTGGACGAGC TCGTGAAAGT GATGGGCCGG CACAAGCCCG AGAACATCGT GATCGAAATG 3661 GCCAGAGAGA ACCAGACCAC CCAGAAGGGA CAGAAGAACA GCCGCGAGAG AATGAAGCGG 3721 ATCGAAGAGG GCATCAAAGA GCTGGGCAGC CAGATCCTGA AAGAACACCC CGTGGAAAAC 3781 ACCCAGCTGC AGAACGAGAA GCTGTACCTG TACTACCTGC AGAATGGGCG GGATATGTAC 3841 GTGGACCAGG AACTGGACAT CAACCGGCTG TCCGACTACG ATGTGGACCA TATCGTGCCT 3901 CAGAGCTTTC TGAAGGACGA CTCCATCGAC AACAAGGTGC TGACCAGAAG CGACAAGAAC 3961 CGGGGCAAGA GCGACAACGT GCCCTCCGAA GAGGTCGTGA AGAAGATGAA GAACTACTGG 4021 CGGCAGCTGC TGAACGCCAA GCTGATTACC CAGAGAAAGT TCGACAATCT GACCAAGGCC 4081 GAGAGAGGCG GCCTGAGCGA ACTGGATAAG GCCGGCTTCA TCAAGAGACA GCTGGTGGAA 4141 ACCCGGCAGA TCACAAAGCA CGTGGCACAG ATCCTGGACT CCCGGATGAA CACTAAGTAC 4201 GACGAGAATG ACAAGCTGAT CCGGGAAGTG AAAGTGATCA CCCTGAAGTC CAAGCTGGTG 4261 TCCGATTTCC GGAAGGATTT CCAGTTTTAC AAAGTGCGCG AGATCAACAA CTACCACCAC 4321 GCCCACGACG CCTACCTGAA CGCCGTCGTG GGAACCGCCC TGATCAAAAA GTACCCTAAG 4381 CTGGAAAGCG AGTTCGTGTA CGGCGACTAC AAGGTGTACG ACGTGCGGAA GATGATCGCC 4441 AAGAGCGAGC AGGAAATCGG CAAGGCTACC GCCAAGTACT TCTTCTACAG CAACATCATG 4501 AACTTTTTCA AGACCGAGAT TACCCTGGCC AACGGCGAGA TCCGGAAGCG GCCTCTGATC 4561 GAGACAAACG GCGAAACCGG GGAGATCGTG TGGGATAAGG GCCGGGATTT TGCCACCGTG 4621 CGGAAAGTGC TGAGCATGCC CCAAGTGAAT ATCGTGAAAA AGACCGAGGT GCAGACAGGC 4681 GGCTTCAGCA AAGAGTCTAT CCTGCCCAAG AGGAACAGCG ATAAGCTGAT CGCCAGAAAG 4741 AAGGACTGGG ACCCTAAGAA GTACGGCGGC TTCGACAGCC CCACCGTGGC CTATTCTGTG 4801 CTGGTGGTGG CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC TGAAGAGTGT GAAAGAGCTG 4861 CTGGGGATCA CCATCATGGA AAGAAGCAGC TTCGAGAAGA ATCCCATCGA CTTTCTGGAA 4921 GCCAAGGGCT ACAAAGAAGT GAAAAAGGAC CTGATCATCA AGCTGCCTAA GTACTCCCTG 4981 TTCGAGCTGG AAAACGGCCG GAAGAGAATG CTGGCCTCTG CCGGCGAACT GCAGAAGGGA 5041 AACGAACTGG CCCTGCCCTC CAAATATGTG AACTTCCTGT ACCTGGCCAG CCACTATGAG 5101 AAGCTGAAGG GCTCCCCCGA GGATAATGAG CAGAAACAGC TGTTTGTGGA ACAGCACAAG 5161 CACTACCTGG ACGAGATCAT CGAGCAGATC AGCGAGTTCT CCAAGAGAGT GATCCTGGCC 5221 GACGCTAATC TGGACAAAGT GCTGTCCGCC TACAACAAGC ACCGGGATAA GCCCATCAGA 5281 GAGCAGGCCG AGAATATCAT CCACCTGTTT ACCCTGACCA ATCTGGGAGC CCCTGCCGCC 5341 TTCAAGTACT TTGACACCAC CATCGACCGG AAGAGGTACA CCAGCACCAA AGAGGTGCTG 5401 GACGCCACCC TGATCCACCA GAGCATCACC GGCCTGTACG AGACACGGAT CGACCTGTCT 5461 CAGCTGGGAG GCGACAAAAG GCCGGCGGCC ACGAAAAAGG CCGGCCAGGC AAAAAAGAAA 5521 AAGgaattcG GCTCCGGCGC AACAAACTTC TCTCTGCTGA AACAAGCCGG AGATGTCGAA 5581 GAGAATCCTG GACCGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 5641 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 5701 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 5761 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 5821 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 5881 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 5941 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 6001 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 6061 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 6121 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 6181 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 6241 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 6301 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 6361 TATCGCCTTC TTGACGAGTT CTTCTGAgaa ttctaaCTAG AGCTCGCTGA TCAGCCTCGA 6421 CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC 6481 TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC 6541 TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT 6601 GGGAAGAgAA TAGCAGGCAT GCTGGGGAgc ggccgcagga acccctagtg atggagttgg 6661 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 6721 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc 6781 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa 6841 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 6901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 6961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 7021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc 7081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 7141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc 7201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 7261 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct 7321 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 7381 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 7441 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 7501 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 7561 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 7621 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 7681 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 7741 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 7801 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 7861 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 7921 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 7981 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 8041 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 8101 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 8161 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 8221 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 8281 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 8341 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 8401 tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 8461 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 8521 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 8581 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 8641 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 8701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 8761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 8821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 8881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 8941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 9001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 9061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 9121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 9181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 9241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 9301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 9361 ttttgctcac atgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.