pPEA3-TA-Luc plasmid

pPEA3-TA-Luc plasmid

PVT92703

pPEA3-TA-Luc plasmid Information

Plasmid name: pPEA3-TA-Luc
Alias: -
Gene lenght: 30bp
Host: Mammalian cells
Use(s): Transcription factor reports
Fragment type: Element
Species: Non
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: minP
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: Fluc
Copy number: Low
Induction:

pPEA3-TA-Luc plasmid Description

pPEA3-TA-Luc is a plasmid suitable for transcription factor reporting in mammalian cells.

pPEA3-TA-Luc plasmid Sequence

1 GGCCTAACTG GCCGGTACCA GGAAGAGGAA GAGGAAGAGG AAGAGGAAGA GATCTGCAGA 61 AGCTTAGACA CTAGAGGGTA TATAATGGAA GCTCGACTTC CAGCTTGGCA ATCCGGTACT 121 GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC 181 CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG 241 GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG 301 TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC 361 CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC 421 CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG 481 AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG 541 ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG 601 ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC 661 TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC 721 ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT 781 TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC 841 GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC 901 TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC 961 AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC 1021 GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC 1081 GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC 1141 ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG 1201 GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC 1261 TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC 1321 ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC 1381 GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG 1441 GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG 1501 AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC 1561 GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG 1621 AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT 1681 GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC 1741 CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGTAATA ATTCTAGAGT 1801 CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC 1861 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA 1921 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 1981 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT 2041 GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT TCCGCTGATC TGCGCAGCAC 2101 CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA 2161 ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 2221 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 2281 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 2341 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG 2401 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC 2461 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG 2521 ACACTAGCGC CACCATGATC GAACAAGACG GCCTCCATGC TGGCAGTCCC GCAGCTTGGG 2581 TCGAACGCTT GTTCGGGTAC GACTGGGCCC AGCAGACCAT CGGATGTAGC GATGCGGCCG 2641 TGTTCCGTCT AAGCGCTCAA GGCCGGCCCG TGCTGTTCGT GAAGACCGAC CTGAGCGGCG 2701 CCCTGAACGA GCTTCAAGAC GAGGCTGCCC GCCTGAGCTG GCTGGCCACC ACCGGCGTAC 2761 CCTGCGCCGC TGTGTTGGAT GTTGTGACCG AAGCCGGCCG GGACTGGCTG CTGCTGGGCG 2821 AGGTCCCTGG CCAGGATCTG CTGAGCAGCC ACCTTGCCCC CGCTGAGAAG GTTTCTATCA 2881 TGGCCGATGC AATGCGGCGC CTGCACACCC TGGACCCCGC TACCTGCCCC TTCGACCACC 2941 AGGCTAAGCA TCGGATCGAG CGTGCTCGGA CCCGCATGGA GGCCGGCCTG GTGGACCAGG 3001 ACGACCTGGA CGAGGAGCAT CAGGGCCTGG CCCCCGCTGA ACTGTTCGCC CGACTGAAAG 3061 CCCGCATGCC GGACGGTGAG GACCTGGTTG TCACACACGG AGATGCCTGC CTCCCTAACA 3121 TCATGGTCGA GAATGGCCGC TTCTCCGGCT TCATCGACTG CGGTCGCCTA GGAGTTGCCG 3181 ACCGCTACCA GGACATCGCC CTGGCCACCC GCGACATCGC TGAGGAGCTT GGCGGCGAGT 3241 GGGCCGACCG CTTCTTAGTC TTGTACGGCA TCGCAGCTCC CGACAGCCAG CGCATCGCCT 3301 TCTACCGCTT GCTCGACGAG TTCTTTTAAT GATCTAGAAC CGGTCATGGC CGCAATAAAA 3361 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGTTCGAACT AGATGCTGTC 3421 GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA 3481 CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG 3541 CAGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 3601 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 3661 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 3721 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 3781 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 3841 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 3901 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 3961 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 4021 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 4081 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 4141 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 4201 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 4261 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 4321 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 4381 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 4441 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCGG CCGCAAATGC 4501 TAAACCACTG CAGTGGTTAC CAGTGCTTGA TCAGTGAGGC ACCGATCTCA GCGATCTGCC 4561 TATTTCGTTC GTCCATAGTG GCCTGACTCC CCGTCGTGTA GATCACTACG ATTCGTGAGG 4621 GCTTACCATC AGGCCCCAGC GCAGCAATGA TGCCGCGAGA GCCGCGTTCA CCGGCCCCCG 4681 ATTTGTCAGC AATGAACCAG CCAGCAGGGA GGGCCGAGCG AAGAAGTGGT CCTGCTACTT 4741 TGTCCGCCTC CATCCAGTCT ATGAGCTGCT GTCGTGATGC TAGAGTAAGA AGTTCGCCAG 4801 TGAGTAGTTT CCGAAGAGTT GTGGCCATTG CTACTGGCAT CGTGGTATCA CGCTCGTCGT 4861 TCGGTATGGC TTCGTTCAAC TCTGGTTCCC AGCGGTCAAG CCGGGTCACA TGATCACCCA 4921 TATTATGAAG AAATGCAGTC AGCTCCTTAG GGCCTCCGAT CGTTGTCAGA AGTAAGTTGG 4981 CCGCGGTGTT GTCGCTCATG GTAATGGCAG CACTACACAA TTCTCTTACC GTCATGCCAT 5041 CCGTAAGATG CTTTTCCGTG ACCGGCGAGT ACTCAACCAA GTCGTTTTGT GAGTAGTGTA 5101 TACGGCGACC AAGCTGCTCT TGCCCGGCGT CTATACGGGA CAACACCGCG CCACATAGCA 5161 GTACTTTGAA AGTGCTCATC ATCGGGAATC GTTCTTCGGG GCGGAAAGAC TCAAGGATCT 5221 TGCCGCTATT GAGATCCAGT TCGATATAGC CCACTCTTGC ACCCAGTTGA TCTTCAGCAT 5281 CTTTTACTTT CACCAGCGTT TCGGGGTGTG CAAAAACAGG CAAGCAAAAT GCCGCAAAGA 5341 AGGGAATGAG TGCGACACGA AAATGTTGGA TGCTCATACT CGTCCTTTTT CAATATTATT 5401 GAAGCATTTA TCAGGGTTAC TAGTACGTCT CTCAAGGATA AGTAAGTAAT ATTAAGGTAC 5461 GGGAGGTATT GGACAGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT 5521 TTTTTGTGTG AATCGATAGT ACTAACATAC GCTCTCCATC AAAACAAAAC GAAACAAAAC 5581 AAACTAGCAA AATAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT TCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.