pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-

pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-Klb-IRES-Puro plasmid

PVT92678

pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-Klb-IRES-Puro plasmid Information

Plasmid name: pLV3-EF1a-GAL4BD-P2A-Elk1(mouse)-PGK-Klb(mouse)-IRES-Puro
Alias: NM_007922.5;Elk-1/NM_031180.2;betaKlotho
Gene lenght: 1290/3132bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pIRES2-EGFP.P3'
Copy number: High
Induction:

pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-Klb-IRES-Puro plasmid Description

pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-Klb-IRES-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-GAL4BD-P2A-Elk1-PGK-Klb-IRES-Puro plasmid Sequence

1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca 3421 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct 3481 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa 3541 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga 3601 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag 3661 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc 3721 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag 3781 gtcaaagaca gttgactgta tcgGCCACCA ACTTCAGCCT GCTGAAGCAG GCCGGCGACG 3841 TGGAGGAGAA CCCCGGCCCC GCCACCatgg acccatctgt gacgctgtgg cagtttctgc 3901 tgcagcttct gagagaacaa ggtaatggcc acatcatctc ctggacctca cgggatggtg 3961 gtgagttcaa gttggtggat gcagaggagg tggcccggct atggggactg cgcaagaaca 4021 agaccaacat gaattacgac aagcttagcc gggccttgcg gtactactat gataagaata 4081 tcatccgcaa ggtgagcggc cagaagtttg tctacaagtt tgtgtcctac ccagaggttg 4141 cagggtgctc cactgaagac tgcccacccc agcctgaggt gtctgtaacc tcggccatag 4201 ccatggcccc tgctactgtc catgcaggcc caggggacac tgccactgga aagccaggaa 4261 caccaaaggg tgcaggaatg acaggccaag gtggcttagc acgaagcagc cggaatgaat 4321 acatgcgctc gggcctctat tctaccttca caatacaatc cctgcagcca cagccacagc 4381 cacccattcc tcctcggcct gcctcagtgc ttcccaacac tacccctgca ggagtaccag 4441 cacccgcctc agggagcagg agcaccagtc caaacccctt agaagcctgt ttggaagcag 4501 aagaggctgg tctgcccctg caggttatcc taaccccacc agaggcccca aaccagaaat 4561 ccgaagagtt gagtctggac ccaagttttg gccatccaca gcccccagaa gtcaaagtgg 4621 aggggcctaa ggaagaattg gaagctgcaa gggctggagg cttcagttca gaagctgtca 4681 aagctgaacc agaagtctca gcctcagaag gcctgctggc tcggctccca gccatcctaa 4741 cagagaacac agcccaggtg tgtggcctct ccacttccac cactgagatc acccaaccgc 4801 agaagggccg aaagcctcgg gacctggaac ttccacttag cccaagcctg ctgggtggcc 4861 agggacctga acggactcca ggatcaggaa caagctctgg tcttcaggca ccggggccag 4921 cgctaacgcc atccctgctc cccacacata ccttgacccc ggtgctgctg acacccagct 4981 cgctgccccc tagcatccat ttctggagca ctctgagtcc aattgcaccc cgtagtccag 5041 ccaagctctc cttccagttt ccgtccagtg gcagcgcaca ggtgcacatc ccttccatca 5101 gtgtggatgg cctctcgacc cccgtggtgc tctccccagg gccccagaag ccatgaGGGT 5161 AGGGGAGGCG CTTTTCCCAA GGCAGTCTGG AGCATGCGCT TTAGCAGCCC CGCTGGGCAC 5221 TTGGCGCTAC ACAAGTGGCC TCTGGCCTCG CACACATTCC ACATCCACCG GTAGGCGCCA 5281 ACCGGCTCCG TTCTTTGGTG GCCCCTTCGC GCCACCTTCT ACTCCTCCCC TAGTCAGGAA 5341 GTTCCCCCCC GCCCCGCAGC TCGCGTCGTG CAGGACGTGA CAAATGGAAG TAGCACGTCT 5401 CACTAGTCTC GTGCAGATGG ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG 5461 CAGCGGCCAA TAGCAGCTTT GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GAAGGGGTGG 5521 GTCCGGGGGC GGGCTCAGGG GCGGGCTCAG GGGCGGGGCG GGCGCCCGAA GGTCCTCCGG 5581 AGGCCCGGCA TTCTGCACGC TTCAAAAGCG CACGTCTGCC GCGCTGTTCT CCTCTTCCTC 5641 ATCTCCGGGC CTTTCGGCCA CCATGAAGAC AGGCTGTGCA GCAGGGTCTC CGGGGAATGA 5701 ATGGATTTTC TTCAGCTCTG ATGAAAGAAA CACACGCTCT AGGAAAACAA TGTCCAACAG 5761 GGCACTGCAA AGATCTGCCG TGCTGTCTGC GTTTGTTCTG CTGCGAGCTG TTACCGGCTT 5821 CTCCGGAGAC GGGAAAGCAA TATGGGATAA AAAACAGTAC GTGAGTCCGG TAAACCCAAG 5881 TCAGCTGTTC CTCTATGACA CTTTCCCTAA AAACTTTTCC TGGGGCGTTG GGACCGGAGC 5941 ATTTCAAGTG GAAGGGAGTT GGAAGACAGA TGGAAGAGGA CCCTCGATCT GGGATCGGTA 6001 CGTCTACTCA CACCTGAGAG GTGTCAACGG CACAGACAGA TCCACTGACA GTTACATCTT 6061 TCTGGAAAAA GACTTGTTGG CTCTGGATTT TTTAGGAGTT TCTTTTTATC AGTTCTCAAT 6121 CTCCTGGCCA CGGTTGTTTC CCAATGGAAC AGTAGCAGCA GTGAATGCGC AAGGTCTCCG 6181 GTACTACCGT GCACTTCTGG ACTCGCTGGT ACTTAGGAAT ATCGAGCCCA TTGTTACCTT 6241 GTACCATTGG GATTTGCCTC TGACGCTCCA GGAAGAATAT GGGGGCTGGA AAAATGCAAC 6301 TATGATAGAT CTCTTCAACG ACTATGCCAC ATACTGCTTC CAGACCTTTG GAGACCGTGT 6361 CAAATATTGG ATTACAATTC ACAACCCTTA CCTTGTTGCT TGGCATGGGT TTGGCACAGG 6421 TATGCATGCA CCAGGAGAGA AGGGAAATTT AACAGCTGTC TACACTGTGG GACACAACCT 6481 GATCAAGGCA CATTCGAAAG TGTGGCATAA CTACGACAAA AACTTCCGCC CTCATCAGAA 6541 GGGTTGGCTC TCCATCACCT TGGGGTCCCA TTGGATAGAG CCAAACAGAA CAGACAACAT 6601 GGAGGACGTG ATCAACTGCC AGCACTCCAT GTCCTCTGTG CTTGGATGGT TCGCCAACCC 6661 CATCCACGGG GACGGCGACT ACCCTGAGTT CATGAAGACG GGCGCCATGA TCCCCGAGTT 6721 CTCTGAGGCA GAGAAGGAGG AGGTGAGGGG CACGGCTGAT TTCTTTGCCT TTTCCTTCGG 6781 GCCCAACAAC TTCAGGCCCT CAAACACCGT GGTGAAAATG GGACAAAATG TATCACTCAA 6841 CTTAAGGCAG GTGCTGAACT GGATTAAACT GGAATACGAT GACCCTCAAA TCTTGATTTC 6901 GGAGAACGGC TGGTTCACAG ATAGCTATAT AAAGACAGAG GACACCACGG CCATCTACAT 6961 GATGAAGAAT TTCCTAAACC AGGTTCTTCA AGCAATAAAA TTTGATGAAA TCCGCGTGTT 7021 TGGTTATACG GCCTGGACTC TCCTGGATGG CTTTGAGTGG CAGGATGCCT ATACGACCCG 7081 ACGAGGGCTG TTTTATGTGG ACTTTAACAG TGAGCAGAAA GAGAGGAAAC CCAAGTCCTC 7141 GGCTCATTAC TACAAGCAGA TCATACAAGA CAACGGCTTC CCTTTGAAAG AGTCCACGCC 7201 AGACATGAAG GGTCGGTTCC CCTGTGATTT CTCTTGGGGA GTCACTGAGT CTGTTCTTAA 7261 GCCCGAGTTT ACGGTCTCCT CCCCGCAGTT TACCGATCCT CACCTGTATG TGTGGAATGT 7321 CACTGGCAAC AGATTGCTCT ACCGAGTGGA AGGGGTAAGG CTGAAAACAA GACCATCCCA 7381 GTGCACAGAT TATGTGAGCA TCAAAAAACG AGTTGAAATG TTGGCAAAAA TGAAAGTCAC 7441 CCACTACCAG TTTGCTCTGG ACTGGACCTC TATCCTTCCC ACTGGCAATC TGTCCAAAGT 7501 TAACAGACAA GTGTTAAGGT ACTATAGGTG TGTGGTGAGC GAAGGACTGA AGCTGGGCGT 7561 CTTCCCCATG GTGACGTTGT ACCACCCAAC CCACTCCCAT CTCGGCCTCC CCCTGCCACT 7621 TCTGAGCAGT GGGGGGTGGC TAAACATGAA CACAGCCAAG GCCTTCCAGG ACTACGCTGA 7681 GCTGTGCTTC CGGGAGTTGG GGGACTTGGT GAAGCTCTGG ATCACCATCA ATGAGCCTAA 7741 CAGGCTGAGT GACATGTACA ACCGCACGAG TAATGACACC TACCGTGCAG CCCACAACCT 7801 GATGATCGCC CATGCCCAGG TCTGGCACCT CTATGATAGG CAGTATAGGC CGGTCCAGCA 7861 TGGGGCTGTG TCGCTGTCCT TACATTGCGA CTGGGCAGAA CCTGCCAACC CCTTTGTGGA 7921 TTCACACTGG AAGGCAGCCG AGCGCTTCCT CCAGTTTGAG ATCGCCTGGT TTGCAGATCC 7981 GCTCTTCAAG ACTGGCGACT ATCCATCGGT TATGAAGGAA TACATCGCCT CCAAGAACCA 8041 GCGAGGGCTG TCTAGCTCAG TCCTGCCGCG CTTCACCGCG AAGGAGAGCA GGCTGGTGAA 8101 GGGTACCGTC GACTTCTACG CACTGAACCA CTTCACTACG AGGTTCGTGA TACACAAGCA 8161 GCTGAACACC AACCGCTCAG TTGCAGACAG GGACGTCCAG TTCCTGCAGG ACATCACCCG 8221 CCTAAGCTCG CCCAGCCGCC TGGCTGTAAC ACCCTGGGGA GTGCGCAAGC TCCTTGCGTG 8281 GATCCGGAGG AACTACAGAG ACAGGGATAT CTACATCACA GCCAATGGCA TCGATGACCT 8341 GGCTCTAGAG GATGATCAGA TCCGAAAGTA CTACTTGGAG AAGTATGTCC AGGAGGCTCT 8401 GAAAGCATAT CTCATTGACA AGGTCAAAAT CAAAGGCTAC TATGCATTCA AACTGACTGA 8461 AGAGAAATCT AAGCCTAGAT TTGGATTTTT CACCTCTGAC TTCAGAGCTA AGTCCTCTGT 8521 CCAGTTTTAC AGCAAGCTGA TCAGCAGCAG TGGCCTCCCC GCTGAGAACA GAAGTCCTGC 8581 GTGTGGTCAG CCTGCGGAAG ACACAGACTG CACCATTTGC TCATTTCTCG TGGAGAAGAA 8641 ACCACTCATC TTCTTCGGTT GCTGCTTCAT CTCCACTCTG GCTGTACTGC TATCCATCAC 8701 CGTTTTTCAT CATCAAAAGA GAAGAAAATT CCAGAAAGCA AGGAACTTAC AAAATATACC 8761 ATTGAAGAAA GGCCACAGCA GAGTTTTCAG CTAAacgcgt cccctctccc tccccccccc 8821 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 8881 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 8941 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 9001 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 9061 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 9121 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 9181 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 9241 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 9301 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 9361 aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa 9421 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 9481 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 9541 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 9601 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 9661 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 9721 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 9781 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 9841 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 9901 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 9961 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaggtcg acaatcaacc 10021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 10081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 10141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 10201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 10261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 10321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 10381 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 10441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 10501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 10561 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta 10621 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 10681 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact 10741 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 10801 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 10861 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 10921 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 10981 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 11041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 11101 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc 11161 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 11221 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 11281 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact 11341 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 11401 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 11461 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag 11521 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 11581 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 11641 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 11701 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 11761 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 11821 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 11881 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 11941 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 12001 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 12061 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 12121 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 12181 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 12241 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 12301 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 12361 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 12421 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 12481 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 12541 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 12601 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 12661 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 12721 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 12781 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 12841 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 12901 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 12961 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 13021 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 13081 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 13141 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 13201 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 13261 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 13321 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 13381 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 13441 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 13501 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 13561 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 13621 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 13681 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 13741 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 13801 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 13861 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 13921 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 13981 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 14041 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 14101 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg 14161 c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.