pCMV-RASA1-3×HA-Neo plasmid

pCMV-RASA1-3×HA-Neo plasmid

PVT92666

pCMV-RASA1-3×HA-Neo plasmid Information

Plasmid name: pCMV-RASA1(human)-3×HA-Neo
Alias: NM_002890.3;CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;p120RASGAP; PKWS; RASA; RASGAP
Gene lenght: 3141bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RASA1-3×HA-Neo plasmid Description

pCMV-RASA1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RASA1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgat ggcggccgag 721 gccggcagtg aggagggcgg cccggtaaca gccggagctg gaggaggcgg cgcggcagcg 781 ggctccagtg cctatcccgc agtgtgtcgg gtgaagatac ccgcggccct gcctgtggca 841 gccgccccct atcctgggct ggtggagacc ggagtggctg gaactctggg tggcggagcc 901 gctttggggt cagagttcct aggagccggg tctgtggcag gggcactggg gggagctgga 961 ctgacagggg gaggtactgc tgctggcgta gctggtgctg ctgctggcgt ggccggtgct 1021 gctgttgctg gacctagtgg agacatggct ctcaccaaac tgcccacttc gttgcttgct 1081 gagactctcg ggccaggcgg cggttttccc cctctgcccc ctccccctta cctgccccct 1141 ttgggggcgg gcctcgggac agtggacgaa ggtgactctc tggatggacc agaatacgag 1201 gaggaagagg tggccatacc gttgaccgct cctccaacta accagtggta tcacggaaaa 1261 cttgacagaa cgatagcaga agaacgcctc aggcaggcag ggaagtctgg cagttatctt 1321 ataagagaga gtgatcggag gccagggtcc tttgtacttt catttcttag ccagatgaat 1381 gttgtcaacc attttaggat tattgctatg tgtggagatt actacattgg tggaagacgt 1441 ttttcttcac tgtcagacct aataggttat tacagtcatg tttcttgttt gcttaaagga 1501 gaaaaattac tttacccagt tgcaccacca gagccagtag aagatagaag gcgtgtacga 1561 gctattctac cttacacaaa agtaccagac actgatgaaa taagtttctt aaaaggagat 1621 atgttcattg ttcataatga attagaagat ggatggatgt gggttacaaa tttaagaaca 1681 gatgaacaag gccttattgt tgaagaccta gtagaagagg tgggccggga agaagatcca 1741 catgaaggaa aaatatggtt ccatgggaag atttccaaac aggaagctta taatttacta 1801 atgacagttg gtcaagtctg cagttttctt gtgaggccct cagataatac tcctggcgat 1861 tattcacttt atttccggac caatgaaaat attcagcgat ttaaaatatg tccaacgcca 1921 aacaatcagt ttatgatggg aggccggtat tataacagca ttggggacat catagatcac 1981 tatcgaaaag aacagattgt tgaaggatat tatcttaagg aacctgtacc aatgcaggat 2041 caagaacaag tactcaatga cacagtggat ggcaaggaaa tctataatac catccgtcgt 2101 aaaacaaagg atgcctttta taaaaacatt gttaagaaag gttatcttct gaaaaagggc 2161 aaaggaaaac gttggaaaaa tttatatttt atcttagagg gtagtgatgc ccaacttatt 2221 tattttgaaa gcgaaaaacg agctaccaaa ccaaaaggat taatagatct cagtgtatgt 2281 tctgtctatg tcgttcatga tagtctcttt ggcaggccaa actgttttca gatagtagtt 2341 cagcacttta gtgaagaaca ttacatcttt tactttgcag gagaaactcc agaacaagca 2401 gaggattgga tgaaaggtct gcaggcattt tgcaatttac ggaaaagtag tccagggaca 2461 tccaataaac gccttcgtca ggtcagcagc cttgttttac atattgaaga agcccataaa 2521 ctcccagtaa aacattttac taatccatat tgtaacatct acctgaatag tgtccaagta 2581 gcaaaaactc atgcaaggga agggcaaaac ccagtatggt cagaagagtt tgtctttgat 2641 gatcttcctc ctgacatcaa tagatttgaa ataactctta gtaataaaac aaagaaaagc 2701 aaagatcctg atatcttatt tatgcgctgc cagttgagcc gattacagaa agggcatgcc 2761 acagatgaat ggtttctgct cagctcccat ataccattaa aaggtattga accagggtcc 2821 ctgcgtgttc gagcacgata ctctatggaa aaaatcatgc cagaagaaga gtacagtgaa 2881 tttaaagagc ttatactgca aaaggaactt catgtagtct atgctttatc acatgtatgt 2941 ggacaagacc gaacactact ggccagcatc ctactgagga tttttcttca cgaaaagctt 3001 gaatcgttgt tgttatgcac actaaatgac agagaaataa gcatggaaga tgaagccact 3061 accctatttc gagccacaac acttgcaagc accttgatgg agcagtatat gaaagccact 3121 gctacacagt ttgttcatca tgctttgaaa gactctattt taaagataat ggaaagcaag 3181 cagtcttgtg agttaagtcc atcaaagtta gaaaaaaatg aagatgtgaa cactaattta 3241 acacacctat tgaacatact ttcagagctt gtggagaaaa tattcatggc ttcagaaata 3301 cttccaccga cattgagata tatttatggg tgtttacaga aatctgttca gcataagtgg 3361 cctacaaata ccaccatgag aacaagagtt gttagtggtt ttgtttttct tcgactcatc 3421 tgtcctgcca tcctgaatcc acggatgttc aatatcatct cagattctcc atctcctatt 3481 gctgcaagaa cactgatatt agtggctaaa tctgtgcaga acttagcaaa tcttgtggaa 3541 tttggagcta aggagcccta catggaaggt gtcaatccat tcatcaaaag caacaaacat 3601 cgtatgatca tgtttttaga tgaacttggg aatgtacctg aacttccgga cactacagag 3661 cattctagaa cggacctgtc ccgtgattta gcagcattgc atgagatttg cgtggctcat 3721 tcagatgaac ttcgaacgct cagtaatgag cgtggtgcac agcagcacgt attgaaaaag 3781 cttctggcta taacagaact gcttcaacaa aaacaaaacc agtatacaaa aaccaatgat 3841 gtcaggCTCG AGTACCCATA CGACGTACCT GATTATGCAA CTTATCCTTA TGATGTACCT 3901 GATTATGCTA GCTATCCTTA TGATGTCCCA GACTACGCAT AGTGAGGGCC CGATATCTCG 3961 ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 4021 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 4081 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 4141 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 4201 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 4261 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 4321 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC 4381 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 4441 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 4501 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG 4561 GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC 4621 AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC 4681 GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC 4741 GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG 4801 CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT 4861 ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 4921 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 4981 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC 5041 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 5101 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 5161 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 5221 ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 5281 AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 5341 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 5401 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 5461 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 5521 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC 5581 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 5641 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT 5701 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 5761 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 5821 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 5881 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 5941 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 6001 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 6061 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 6121 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 6181 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC 6241 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 6301 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 6361 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 6421 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 6481 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC 6541 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 6601 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 6661 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 6721 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 6781 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 6841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 6901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 6961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 7021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 7081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 7141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 7201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 7261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 7321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 7381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 7441 AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 7501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 7561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 7621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 7681 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 7741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 9001 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA 9061 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 9121 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 9181 GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.