pLV3-CMV-3×FLAG-CANX-CopGFP-EF

pLV3-CMV-3×FLAG-CANX-CopGFP-EF1a-Blast plasmid

PVT91913

pLV3-CMV-3×FLAG-CANX-CopGFP-EF1a-Blast plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-CANX(human)-CopGFP-EF1a-Blast
Alias: NM_001746.4;CNX; IP90; P90;
Gene lenght: 1776bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-CANX-CopGFP-EF1a-Blast plasmid Description

pLV3-CMV-3×FLAG-CANX-CopGFP-EF1a-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-CANX-CopGFP-EF1a-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAgccacca tgGACTACAA GGATGACGAT GACAAGGATT ACAAAGACGA CGATGATAAG 2341 GACTATAAGG ATGATGACGA CAAAgaattc atggaaggga agtggttgct gtgtatgtta 2401 ctggtgcttg gaactgctat tgttgaggct catgatggac atgatgatga tgtgattgat 2461 attgaggatg accttgacga tgtcattgaa gaggtagaag actcaaaacc agataccact 2521 gctcctcctt catctcccaa ggttacttac aaagctccag ttccaacagg ggaagtatat 2581 tttgctgatt cttttgacag aggaactctg tcagggtgga ttttatccaa agccaagaaa 2641 gacgataccg atgatgaaat tgccaaatat gatggaaagt gggaggtaga ggaaatgaag 2701 gagtcaaagc ttccaggtga taaaggactt gtgttgatgt ctcgggccaa gcatcatgcc 2761 atctctgcta aactgaacaa gcccttcctg tttgacacca agcctctcat tgttcagtat 2821 gaggttaatt tccaaaatgg aatagaatgt ggtggtgcct atgtgaaact gctttctaaa 2881 acaccagaac tcaacctgga tcagttccat gacaagaccc cttatacgat tatgtttggt 2941 ccagataaat gtggagagga ctataaactg cacttcatct tccgacacaa aaaccccaaa 3001 acgggtatct atgaagaaaa acatgctaag aggccagatg cagatctgaa gacctatttt 3061 actgataaga aaacacatct ttacacacta atcttgaatc cagataatag ttttgaaata 3121 ctggttgacc aatctgtggt gaatagtgga aatctgctca atgacatgac tcctcctgta 3181 aatccttcac gtgaaattga ggacccagaa gaccggaagc ccgaggattg ggatgaaaga 3241 ccaaaaatcc cagatccaga agctgtcaag ccagatgact gggatgaaga tgcccctgct 3301 aagattccag atgaagaggc cacaaaaccc gaaggctggt tagatgatga gcctgagtac 3361 gtacctgatc cagacgcaga gaaacctgag gattgggatg aagacatgga tggagaatgg 3421 gaggctcctc agattgccaa ccctagatgt gagtcagctc ctggatgtgg tgtctggcag 3481 cgacctgtga ttgacaaccc caattataaa ggcaaatgga agcctcctat gattgacaat 3541 cccagttacc agggaatctg gaaacccagg aaaataccaa atccagattt ctttgaagat 3601 ctggaacctt tcagaatgac tccttttagt gctattggtt tggagctgtg gtccatgacc 3661 tctgacattt tttttgacaa ctttatcatt tgtgctgatc gaagaatagt tgatgattgg 3721 gccaatgatg gatggggcct gaagaaagct gctgatgggg ctgctgagcc aggcgttgtg 3781 gggcagatga tcgaggcagc tgaagagcgc ccgtggctgt gggtagtcta tattctaact 3841 gtagcccttc ctgtgttcct ggttatcctc ttctgctgtt ctggaaagaa acagaccagt 3901 ggtatggagt ataagaaaac tgatgcacct caaccggatg tgaaggaaga ggaagaagag 3961 aaggaagagg aaaaggacaa gggagatgag gaggaggaag gagaagagaa acttgaagag 4021 aaacagaaaa gtgatgctga agaagatggt ggcactgtca gtcaagagga ggaagacaga 4081 aaacctaaag cagaggagga tgaaattttg aacagatcac caagaaacag aaagccacga 4141 agagagggag gagggggatc aggaggcggt ggatctatgg agagcgacga gagcggcctg 4201 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4261 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4321 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4381 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4441 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4501 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4561 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4621 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4681 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4741 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4801 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4861 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 4921 accggatctc gcTAGGATCC GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG 4981 TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 5041 ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 5101 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 5161 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT 5221 CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG 5281 CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC 5341 AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC 5401 TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG 5461 CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG GCCACCATGG CCAAGCCTTT 5521 GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT 5581 CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG 5641 TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC 5701 TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG 5761 CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA 5821 AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC 5881 CTCTGGTTAT GTGTGGGAGG GCTAAGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 5941 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 6001 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 6061 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 6121 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 6181 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 6241 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 6301 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 6361 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 6421 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 6481 CCTTTGGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG 6541 ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA 6601 AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG 6661 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC 6721 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT 6781 TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT 6841 TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA 6901 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 6961 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT 7021 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 7081 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 7141 CTTTTTTGGA GGCCTAGACT TTTGCAGAGA CGGCCCAAAT TCGTAATCAT GGTCATAGCT 7201 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 7261 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 7321 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 7381 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 7441 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 7501 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 7561 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 7621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 7681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 7741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 7801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 7861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 7921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 7981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 8041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 8101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 8161 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 8221 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 8281 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 8341 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 8401 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 8461 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 8521 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 8581 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 8641 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 8701 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 8761 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 8821 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 8881 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 8941 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 9001 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 9061 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 9121 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 9181 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 9241 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 9301 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 9361 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG 9421 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 9481 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 9541 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 9601 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 9661 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 9721 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 9781 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.