pLV3-CMV-Otub1-3×FLAG-CopGFP-P

pLV3-CMV-Otub1-3×FLAG-CopGFP-Puro plasmid

PVT91906

pLV3-CMV-Otub1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Otub1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_134150.2;
Gene lenght: 813bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Otub1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Otub1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Otub1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cggcggagga acctcagcag 2341 cagaagcagg agccactggg tagcgactcc gaaggtgtta actgtttggc ctatgatgaa 2401 gccattatgg ctcagcagga ccgaattcag caagagattg ctgtgcagaa tcctctggtg 2461 tcagagcggc tggaactctc agtcctgtac aaggagtatg ctgaggatga caacatctac 2521 caacagaaga tcaaggacct ccacaaaaag tactcataca tacggaagac ccggcctgat 2581 ggcaactgct tctaccgagc gtttggcttc tcccacctgg aggcactgct agatgacagc 2641 aaggaactgc agcggttcaa agccgtgtct gccaagagta aagaggacct ggtatcccag 2701 ggcttcactg aattcacaat tgaagacttc cacaacacgt tcatggacct tatcgagcag 2761 gtggagaagc agacctcagt agctgacctg ctggcctcct tcaatgacca gagcacctca 2821 gactaccttg tggtctacct gcgactgctc acctcaggct acctacagcg ggagagcaag 2881 ttcttcgagc acttcatcga gggtggccgg actgttaagg agttctgcca gcaggaggtg 2941 gaacccatgt gcaaggagag cgaccacatc cacatcatag ccctggccca ggccctcagt 3001 gtgtccatcc aagtggagta catggaccgc ggcgagggcg gcaccaccaa cccacacgtc 3061 ttccccgagg gctccgagcc caaggtctac cttctctacc gacctggaca ctacgatatc 3121 ctctacaaag gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC 3181 TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc cggtgcccgt 3241 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 3301 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 3361 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 3421 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 3481 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 3541 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 3601 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 3661 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 3721 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 3781 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 3841 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 3901 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 3961 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 4021 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 4081 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 4141 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 4201 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 4261 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 4321 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 4381 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 4441 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 4501 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 4561 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 4621 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4681 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 4741 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 4801 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 4861 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 4921 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 4981 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5041 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5101 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5161 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 5221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 5521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaATCA 5581 TCGTCCTTTC CTtggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 5641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 5701 ctgcggcctc ttccgcgtct Tcgccttcgc cctcagacga gtcggatctc cctttggGcc 5761 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 5821 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa Gataagatct 5881 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 5941 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 6001 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 6061 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6121 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 6181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6241 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctaT CCCGCCCCTA 6301 ACTCCGCCCA tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6361 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 6421 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc 6481 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6541 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6601 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6661 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6721 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 6841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 6901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 7081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 7141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 7201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 7261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 7321 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 7381 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 7441 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7501 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7561 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7621 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7681 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7741 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7801 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 7861 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 7921 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7981 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 8041 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 8101 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 8161 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 8221 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 8281 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 8341 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 8401 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 8461 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8521 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 8581 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 8641 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 8701 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 8761 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 8821 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 8881 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 8941 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 9001 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 9061 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.