pCMV-HA-RIPK1 plasmid

pCMV-HA-RIPK1 plasmid

PVT91887

pCMV-HA-RIPK1 plasmid Information

Plasmid name: pCMV-HA-RIPK1(human)
Alias: NM_003804.6;AIEFL; IMD57; RIP; RIP-1; RIP1
Gene lenght: 2013bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pCMV-HA-RIPK1 plasmid Description

pCMV-HA-RIPK1 is a plasmid suitable for protein expression in mammalian cells.

pCMV-HA-RIPK1 plasmid Sequence

1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG 181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA 541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC 601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG 661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG 721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA 781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC 841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTgccaccat gcaaccagac 901 atgtccttga atgtcattaa gatgaaatcc agtgacttcc tggagagtgc agaactggac 961 agcggaggct ttgggaaggt gtctctgtgt ttccacagaa cccagggact catgatcatg 1021 aaaacagtgt acaaggggcc caactgcatt gagcacaacg aggccctctt ggaggaggcg 1081 aagatgatga acagactgag acacagccgg gtggtgaagc tcctgggcgt catcatagag 1141 gaagggaagt actccctggt gatggagtac atggagaagg gcaacctgat gcacgtgctg 1201 aaagccgaga tgagtactcc gctttctgta aaaggaagga taattttgga aatcattgaa 1261 ggaatgtgct acttacatgg aaaaggcgtg atacacaagg acctgaagcc tgaaaatatc 1321 cttgttgata atgacttcca cattaagatc gcagacctcg gccttgcctc ctttaagatg 1381 tggagcaaac tgaataatga agagcacaat gagctgaggg aagtggacgg caccgctaag 1441 aagaatggcg gcaccctcta ctacatggcg cccgagcacc tgaatgacgt caacgcaaag 1501 cccacagaga agtcggatgt gtacagcttt gctgtagtac tctgggcgat atttgcaaat 1561 aaggagccat atgaaaatgc tatctgtgag cagcagttga taatgtgcat aaaatctggg 1621 aacaggccag atgtggatga catcactgag tactgcccaa gagaaattat cagtctcatg 1681 aagctctgct gggaagcgaa tccggaagct cggccgacat ttcctggcat tgaagaaaaa 1741 tttaggcctt tttatttaag tcaattagaa gaaagtgtag aagaggacgt gaagagttta 1801 aagaaagagt attcaaacga aaatgcagtt gtgaagagaa tgcagtctct tcaacttgat 1861 tgtgtggcag taccttcaag ccggtcaaat tcagccacag aacagcctgg ttcactgcac 1921 agttcccagg gacttgggat gggtcctgtg gaggagtcct ggtttgctcc ttccctggag 1981 cacccacaag aagagaatga gcccagcctg cagagtaaac tccaagacga agccaactac 2041 catctttatg gcagccgcat ggacaggcag acgaaacagc agcccagaca gaatgtggct 2101 tacaacagag aggaggaaag gagacgcagg gtctcccatg acccttttgc acagcaaaga 2161 ccttacgaga attttcagaa tacagaggga aaaggcactg cttattccag tgcagccagt 2221 catggtaatg cagtgcacca gccctcaggg ctcaccagcc aacctcaagt actgtatcag 2281 aacaatggat tatatagctc acatggcttt ggaacaagac cactggatcc aggaacagca 2341 ggtcccagag tttggtacag gccaattcca agtcatatgc ctagtctgca taatatccca 2401 gtgcctgaga ccaactatct aggaaataca cccaccatgc cattcagctc cttgccacca 2461 acagatgaat ctataaaata taccatatac aatagtactg gcattcagat tggagcctac 2521 aattatatgg agattggtgg gacgagttca tcactactag acagcacaaa tacgaacttc 2581 aaagaagagc cagctgctaa gtaccaagct atctttgata ataccactag tctgacggat 2641 aaacacctgg acccaatcag ggaaaatctg ggaaagcact ggaaaaactg tgcccgtaaa 2701 ctgggcttca cacagtctca gattgatgaa attgaccatg actatgagcg agatggactg 2761 aaagaaaagg tttaccagat gctccaaaag tgggtgatga gggaaggcat aaagggagcc 2821 acggtgggga agctggccca ggcgctccac cagtgttcca ggatcgacct tctgagcagc 2881 ttgatttacg tcagccagaa ctaaGGTACC GCGGCCGCGG GGATCCAGAC ATGATAAGAT 2941 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 3001 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 3061 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTCGG 3121 ATCCTCTAGA GTCGATCTGC AGGCATGCTA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 3181 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 3241 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 3301 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 3361 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 3421 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3481 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 3541 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 3601 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 3661 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 3721 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 3781 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 3841 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 3901 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 3961 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 4021 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4081 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4141 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4201 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4261 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 4321 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 4381 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 4441 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 4501 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 4561 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 4621 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 4681 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 4741 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 4801 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 4861 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 4921 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 4981 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 5041 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 5101 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 5161 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 5221 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 5281 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA 5341 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG 5401 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT 5461 AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC 5521 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT 5581 GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA 5641 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG 5701 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC 5761 GACGTTGTAA AACGACGGCC AGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.