pLV3-CMV-TRIM28-3×FLAG-Hyg pla

pLV3-CMV-TRIM28-3×FLAG-Hyg plasmid

PVT91639

pLV3-CMV-TRIM28-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-TRIM28(human)-3×FLAG-Hyg
Alias: NM_005762.3;KAP1; PPP1R157; RNF96; TF1B; TIF1B;TIF1beta;
Gene lenght: 2505bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM28-3×FLAG-Hyg plasmid Description

pLV3-CMV-TRIM28-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TRIM28-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggcggc ctccgcggcg gcagcctcgg cagcagcggc 2341 ctcggccgcc tctggcagcc cgggcccggg cgagggctcc gctggcggcg aaaagcgctc 2401 caccgcccct tcggccgcag cctcggcctc tgcctcagcc gcggcgtcgt cgcccgcggg 2461 gggcggcgcc gaggcgctgg agctgctgga gcactgcggc gtgtgcagag agcgcctgcg 2521 acccgagagg gagccccgcc tgctgccctg tttgcactcg gcctgtagtg cctgcttagg 2581 gcccgcggcc cccgccgccg ccaacagctc gggggacggc ggggcggcgg gcgacggcac 2641 cgtggtggac tgtcccgtgt gcaagcaaca gtgcttctcc aaagacatcg tggagaatta 2701 tttcatgcgt gatagtggca gcaaggctgc caccgacgcc caggatgcga accagtgctg 2761 cactagctgt gaggataatg ccccagccac cagctactgt gtggagtgct cggagcctct 2821 gtgtgagacc tgtgtagagg cgcaccagcg ggtgaagtac accaaggacc atactgtgcg 2881 ctctactggg ccagccaagt ctcgggatgg tgaacgtact gtctattgca acgtacacaa 2941 gcatgaaccc cttgtgctgt tttgtgagag ctgtgatact ctcacctgcc gagactgcca 3001 gctcaatgcc cacaaggacc accagtacca gttcttagag gatgcagtga ggaaccagcg 3061 caagctcctg gcctcactgg tgaagcgcct tggggacaaa catgcaacat tgcagaagag 3121 caccaaggag gttcgcagct caatccgcca ggtgtctgac gtacagaagc gtgtgcaagt 3181 ggatgtcaag atggccatcc tgcagatcat gaaggagctg aataagcggg gccgtgtgct 3241 ggtcaatgat gcccagaagg tgactgaggg gcagcaggag cgcctggagc ggcagcactg 3301 gaccatgacc aagatccaga agcaccagga gcacattctg cgctttgcct cttgggctct 3361 ggagagtgac aacaacacag cccttttgct ttctaagaag ttgatctact tccagctgca 3421 ccgggccctc aagatgattg tggatcccgt ggagccacat ggcgagatga agtttcagtg 3481 ggacctcaat gcctggacca agagtgccga ggcctttggc aagattgtgg cagagcgtcc 3541 tggcactaac tcaacaggcc ctgcacccat ggcccctcca agagccccag ggcccctgag 3601 caagcagggc tctggcagca gccagcccat ggaggtgcag gaaggctatg gctttgggtc 3661 aggagatgat ccctactcaa gtgcagagcc ccatgtgtca ggtgtgaaac ggtcccgctc 3721 aggtgagggc gaggtgagcg gccttatgcg caaggtgcca cgagtgagcc ttgaacgcct 3781 ggacctggac ctcacagctg acagccagcc acccgtcttc aaggtcttcc caggcagtac 3841 cactgaggac tacaacctta ttgttattga acgtggcgct gccgctgcag ctaccggcca 3901 gccagggact gcgcctgcag gaacccctgg tgccccaccc ctggctggca tggccattgt 3961 caaggaggag gagacggagg ctgccattgg agcccctcct actgccactg agggccctga 4021 gaccaaacct gtgcttatgg ctcttgcgga gggtcctggt gctgagggtc cccgcctggc 4081 ctcacctagt ggcagcacca gctcagggct ggaggtggtg gctcctgagg gtacctcagc 4141 cccaggtggt ggcccgggaa ccctggatga cagtgccacc atttgccgtg tctgccagaa 4201 gccaggcgat ctggttatgt gcaaccagtg tgagttttgt ttccacctgg actgtcacct 4261 gccggccctg caggatgtac caggggagga gtggagctgc tcactctgcc atgtgctccc 4321 tgacctgaag gaggaggatg gcagcctcag cctggatggt gcagacagca ctggcgtggt 4381 ggccaagctc tcaccagcca accagcggaa atgtgagcgt gtactgctgg ccctattctg 4441 tcacgaaccc tgccgccccc tgcatcagct ggctaccgac tccaccttct ccctggacca 4501 gcccggtggc accctggatc tgaccctgat ccgtgcccgc ctccaggaga agttgtcacc 4561 tccctacagc tccccacagg agtttgccca ggatgtgggc cgcatgttca agcaattcaa 4621 caagttaact gaggacaagg cagacgtgca gtccatcatc ggcctgcagc gcttcttcga 4681 gacgcgcatg aacgaggcct tcggtgacac caagttctct gctgtgctgg tggagccccc 4741 gccgatgagc ctgcctggtg ctggcctgag ttcccaggag ctgtctggtg gccctggtga 4801 tggccccCTC GAGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 4861 CAAGGATGAC GATGACAAGT AGcggccgca aggatctgcg atcgctccgg tgcccgtcag 4921 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4981 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 5041 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 5101 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 5161 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 5221 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 5281 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 5341 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 5401 cgccgttaca gatccaagct gtgaccggcg cctacgtcga catgaaaaag cctgaactca 5461 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc 5521 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg 5581 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact 5641 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc 5701 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg 5761 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc 5821 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat 5881 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg 5941 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg 6001 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg 6061 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat 6121 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc 6181 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc 6241 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag 6301 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta 6361 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg 6421 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataggtc gacaatcaac 6481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 6541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 6601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 6661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 6721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 6781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 6841 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 6901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 6961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 7021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc tttaagacca 7081 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 7141 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 7201 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 7261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 7321 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 7381 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 7441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 7501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 7561 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 7681 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 7741 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7801 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7861 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7921 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7981 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 8041 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 8101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 8161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 8221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 8281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 8341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 8401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 8461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 8521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 8581 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8761 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8821 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 9001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 9061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 9121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 9181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 9241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 9301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 9361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 9421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 9481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 9541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9901 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 9961 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 10021 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 10081 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 10141 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 10201 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 10261 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 10321 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.