pLV2-CMV-DDX3X-3×HA-Puro plasm

pLV2-CMV-DDX3X-3×HA-Puro plasmid

PVT91635

pLV2-CMV-DDX3X-3×HA-Puro plasmid Information

Plasmid name: pLV2-CMV-DDX3X(human)-3×HA-Puro
Alias: NM_001356.5;CAP-Rf; DBX; DDX14; DDX3; HLP2; MRX102;MRXSSB
Gene lenght: 1986bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-DDX3X-3×HA-Puro plasmid Description

pLV2-CMV-DDX3X-3×HA-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-DDX3X-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatgagtca tgtggcagtg gaaaatgcgc tcgggctgga 2881 ccagcagttt gctggcctag acctgaactc ttcagataat cagagtggag gaagtacagc 2941 cagcaaaggg cgctatattc ctcctcattt aaggaaccga gaagctacta aaggtttcta 3001 cgataaagac agttcagggt ggagttctag caaagataag gatgcgtata gcagttttgg 3061 atctcgtagt gattcaagag ggaagtctag cttcttcagt gatcgtggaa gtggatcaag 3121 gggaaggttt gatgatcgtg gacggagtga ttacgatggc attggcagcc gtggtgacag 3181 aagtggcttt ggcaaatttg aacgtggtgg aaacagtcgc tggtgtgaca aatcagatga 3241 agatgattgg tcaaaaccac tcccaccaag tgaacgcttg gaacaggaac tcttttctgg 3301 aggcaacact gggattaatt ttgagaaata cgatgacatt ccagttgagg caacaggcaa 3361 caactgtcct ccacatattg aaagtttcag tgatgttgag atgggagaaa ttatcatggg 3421 aaacattgag cttactcgtt atactcgccc aactccagtg caaaagcatg ctattcctat 3481 tatcaaagag aaaagagact tgatggcttg tgcccaaaca gggtctggaa aaactgcagc 3541 atttctgttg cccatcttga gtcagattta ttcagatggt ccaggcgagg ctttgagggc 3601 catgaaggaa aatggaaggt atgggcgccg caaacaatac ccaatctcct tggtattagc 3661 accaacgaga gagttggcag tacagatcta cgaggaagcc agaaaatttt cataccgatc 3721 tagagttcgt ccttgcgtgg tttatggtgg tgccgatatt ggtcagcaga ttcgagactt 3781 ggaacgtgga tgccatttgt tagtagccac tccaggacgt ctagtggata tgatggaaag 3841 aggaaagatt ggattagact tttgcaaata cttggtgtta gatgaagctg atcggatgtt 3901 ggatatgggg tttgagcctc agattcgtag aatagtcgaa caagatacta tgcctccaaa 3961 gggtgtccgc cacactatga tgtttagtgc tacttttcct aaggaaatac agatgctggc 4021 tcgtgatttc ttagatgaat atatcttctt ggctgtagga agagttggct ctacctctga 4081 aaacatcaca cagaaagtag tttgggtgga agaatcagac aaacggtcat ttctgcttga 4141 cctcctaaat gcaacaggca aggattcact gaccttagtg tttgtggaga ccaaaaaggg 4201 tgcagattct ctggaggatt tcttatacca tgaaggatac gcatgtacca gcatccatgg 4261 agaccgttct cagagggata gagaagaggc ccttcaccag ttccgctcag gaaaaagccc 4321 aattttagtg gctacagcag tagcagcaag aggactggac atttcaaatg tgaaacatgt 4381 tatcaatttt gacttgccaa gtgatattga agaatatgta catcgtattg gtcgtacggg 4441 acgtgtagga aaccttggcc tggcaacctc attctttaac gagaggaaca taaatattac 4501 taaggatttg ttggatcttc ttgttgaagc taaacaagaa gtgccgtctt ggttagaaaa 4561 catggcttat gaacaccact acaagggtag cagtcgtgga cgttctaaga gtagcagatt 4621 tagtggaggg tttggtgcca gagactaccg acaaagtagc ggtgccagca gttccagctt 4681 cagcagcagc cgcgcaagca gcagccgcag tggcggaggt ggccacggta gcagcagagg 4741 atttggtgga ggtggctatg gaggctttta caacagtgat ggatatggag gaaattataa 4801 ctcccagggg gttgactggt ggggtaacgg atccTACCCA TACGACGTAC CTGATTATGC 4861 AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC 4921 ATAGtctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 4981 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 5041 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 5101 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 5161 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 5221 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 5281 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 5341 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 5401 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc 5461 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5521 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5581 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5641 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5701 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5761 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5821 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5881 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5941 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6001 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6061 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6121 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6181 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6241 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6301 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6361 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6421 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 6481 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6541 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6601 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6661 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 6721 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 6781 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6841 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 6901 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 6961 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 7021 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 7081 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 7141 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 7201 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 7261 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 7321 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 7381 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 7441 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 7501 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 7561 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg 7621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 7681 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 7741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 7801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 7861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 8041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 8101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 8161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 8221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 8281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 8341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 8401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 8461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 8521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 8581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 8641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 8701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 8761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 8821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 8881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 8941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 9001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 9061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 9121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 9181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 9241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 9301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 9361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 9421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 9481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 9541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 9601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 9661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 9721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 9781 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa 9841 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9901 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 9961 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 10021 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 10081 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta 10141 caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.