pLV3-CMV-NFAT5-3×Myc-Neo plasm

pLV3-CMV-NFAT5-3×Myc-Neo plasmid

PVT91618

pLV3-CMV-NFAT5-3×Myc-Neo plasmid Information

Plasmid name: pLV3-CMV-NFAT5(human)-3×Myc-Neo
Alias: NM_138714.4;NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
Gene lenght: 4365bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NFAT5-3×Myc-Neo plasmid Description

pLV3-CMV-NFAT5-3×Myc-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NFAT5-3×Myc-Neo plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgggcggt gcttgcagct cctttaccac ctcttccagc 2341 cctaccattt attctacctc agtcaccgac agcaaggcta tgcaagtgga gagctgctcc 2401 tcagccgtgg gggtaagtaa cagaggggta agtgaaaagc agttaaccag taacacagtt 2461 cagcagcatc catcaacacc gaagaggcac acagtcttgt acatctcacc accacctgag 2521 gacttgctgg ataacagtcg gatgtcctgc caggatgagg ggtgtggatt ggaatctgag 2581 cagagctgca gtatgtggat ggaggattcc ccctccaact tcagtaacat gagcaccagt 2641 tcctacaatg ataacactga ggtacctcgt aaatcacgaa aacgaaatcc aaagcagagg 2701 ccgggggtca aacgacgaga ttgtgaagaa tctaatatgg atatatttga tgccgacagt 2761 gccaaagcac ctcactatgt gctttctcag cttaccacgg acaacaaagg caactcaaaa 2821 gcgggaaatg gaacattgga aaaccaaaaa ggaactggag taaagaagag ccctatgttg 2881 tgtggacaat atcctgttaa aagtgaggga aaggagctga agatagttgt acaacctgag 2941 acacagcacc gagctcggta cctgactgag ggcagccgtg gctcagtgaa agatagaaca 3001 cagcaaggct ttcctacagt aaagctggaa ggccataatg aacctgtagt gttgcaagtg 3061 tttgtgggca acgactctgg acgagtgaaa ccacatggat tttatcaggc ctgcagagta 3121 actggacgaa atacaactcc ttgcaaagaa gtggacattg aaggcactac tgttatagaa 3181 gtcggccttg atcctagcaa caacatgaca ctggcggtgg actgcgtagg gatattgaaa 3241 ttgaggaatg ctgatgtcga agccagaata ggaattgctg gttccaagaa gaaaagcact 3301 cgtgccagat tggtttttcg agttaatatc atgaggaaag atggctccac tttgacactg 3361 caaacaccct cttctccaat tttgtgtact cagccagcag gagtgccaga aatcttaaag 3421 aaaagcttgc atagctgttc agtgaaagga gaagaagaag tgtttttaat cggcaagaac 3481 tttctgaaag gaactaaagt tattttccaa gaaaatgttt ctgatgaaaa ctcttggaag 3541 tcagaagctg aaattgatat ggaactattt catcagaatc atcttattgt gaaggttcct 3601 ccctatcatg accaacatat aactttgcct gtgtcagtgg gaatatatgt agtgacaaat 3661 gctggaagat ctcatgatgt tcaaccattc acttacactc cagacccagc agcagctggt 3721 gctttgaatg taaatgtgaa gaaggaaata tctagtccag caagaccttg ctcttttgaa 3781 gaggccatga aagcaatgaa aactactgga tgtaatttag ataaggtaaa tattatccct 3841 aatgccctga tgactccact cataccaagc agtatgatta agagtgaaga tgttactcca 3901 atggaagtaa cagcagaaaa aagatcttcc actattttta agactacaaa gtctgttgga 3961 tcaactcagc aaacattaga aaacatctca aacatagcag gaaatggctc tttttcatca 4021 ccatcatctt cccacctacc ttctgaaaat gaaaaacagc agcagattca gcccaaggca 4081 tacaacccag agaccctgac aactattcaa acccaggaca tctcacagcc tggtactttt 4141 ccagcagttt ctgcttctag tcagctgccc aacagcgatg cactattgca gcaggctaca 4201 cagtttcaga caagagaaac tcagtctaga gagatattac agtcagatgg tacagtggtt 4261 aatttgtcac aactgactga ggcatcacaa caacagcagc agtcaccact acaagaacaa 4321 gcacagactt tacagcagca gatttcatca aatatttttc catcaccaaa tagtgtgagt 4381 cagcttcaga atactattca gcagctgcaa gcagggagtt tcacaggcag tactgctagt 4441 ggcagcagtg gaagtgttga cttggtccaa caagttttag aggcacagca gcagttatct 4501 tcagttttat tttctgctcc agatggtaat gagaatgttc aagagcagct tagtgcagat 4561 atttttcaac aagtcagtca aattcagagt ggtgtaagcc ctggaatgtt ttcctcaaca 4621 gagccaacag tccataccag accagataat ttattacctg gaagagctga aagtgttcat 4681 ccacagtctg aaaacacgtt atctaatcaa cagcagcagc agcagcagca acagcaagtg 4741 atggaatctt cagccgcaat ggtgatggag atgcaacaga gtatctgcca ggcagctgcc 4801 cagattcagt cagagttatt cccttcaact gcttcagcaa atggaaacct tcagcaatcg 4861 ccagtttacc agcagacttc tcacatgatg agtgcattgt ctaccaatga ggatatgcaa 4921 atgcagtgtg aattgttttc ttctcctcct gcagtttctg gaaatgaaac ttctacaact 4981 accacacagc aggttgcaac ccctggcact accatgtttc agacatcaag ttcaggagat 5041 ggagaagaaa ctggaacaca agcaaaacag attcagaaca gtgtctttca gaccatggtc 5101 caaatgcaac atagtgggga caatcaacct caagttaacc ttttttcatc cacaaaaagt 5161 atgatgagtg ttcagaatag tggtacccaa caacaaggta atggtttatt ccagcaaggg 5221 aatgagatga tgtcacttca atctggaaat tttttgcagc agtcttctca ttcacaggcc 5281 caactttttc atcctcaaaa tcctattgcc gatgctcaga acctttccca ggaaactcaa 5341 ggttctctct ttcatagtcc aaatcctatt gtccacagtc agacttctac aacctcctct 5401 gaacaaatgc agcctccaat gtttcactct caaagtacca ttgctgtgtt acagggctct 5461 tcagttcctc aagaccagca gtcaaccaac atatttcttt cccagagtcc catgaataat 5521 cttcagacta acacagtagc ccaagaagca ttttttgcag caccgaactc aatttctcca 5581 cttcagtcaa catcaaacag tgaacaacaa gctgctttcc aacagcaagc tccaatatca 5641 cacatccaga ctcctatgct ttcccaagaa caggcacaac ccccgcagca gggtttattt 5701 cagcctcagg tggccctggg ctcccttcca cctaatccaa tgcctcaaag ccaacaagga 5761 accatgttcc agtcacagca ctcaatagtt gccatgcaga gtaactctcc atcccaggaa 5821 cagcagcagc agcagcaaca gcagcagcaa cagcagcagc aacaacaaca gagcatttta 5881 ttcagtaatc agaataccat ggctacaatg gcgtctccaa agcaaccacc accaaacatg 5941 atattcaacc caaatcaaaa tccaatggct aatcaggagc aacagaacca gtcaattttt 6001 caccaacaaa gtaacatggc cccaatgaat caagagcaac agcccatgca atttcagagt 6061 cagtccacag tttcctcact tcagaaccca ggtcctaccc agtcggaatc atcacagacc 6121 cccttgttcc atagctctcc tcagattcag ttggtacaag ggtcacctag ttctcaagag 6181 cagcaagtaa ctctcttctt atctccagca tccatgtctg ccttgcagac cagtataaat 6241 caacaagata tgcaacagtc tcctctttat tcccctcaga acaacatgcc tggaattcaa 6301 ggagccacat cttcgcctca accacaggct actttatttc acaacacagc aggaggcaca 6361 atgaaccaac tgcagaattc tcctggctca tctcagcaga catcaggaat gttcttattt 6421 ggcattcaaa ataactgtag tcagctttta acctctggac cagctacatt gcctgatcag 6481 ttgatggcca taagtcagcc aggccaacca caaaacgagg gccagccacc tgtgacaaca 6541 cttctttctc agcaaatgcc agagaattct ccactggcat cctctataaa caccaaccag 6601 aacatcgaaa agattgattt gcttgtttca ttgcaaaacc aagggaacaa cttgactggc 6661 tcctttGGAT CCGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT 6721 GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAGCGGCC GCGAAGGATC 6781 TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG 6841 TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG 6901 GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA 6961 AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG 7021 AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT 7081 CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC 7141 GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG 7201 AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC 7261 GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG 7321 CTAGGCCACC atggctgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 7381 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 7441 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 7501 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 7561 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 7621 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 7681 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 7741 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 7801 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 7861 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 7921 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 7981 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 8041 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 8101 tcgccttctt gacgagttct tctgaGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 8161 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 8221 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 8281 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 8341 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 8401 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 8461 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 8521 GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 8581 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 8641 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 8701 CCTTTGGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG 8761 ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA 8821 AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG 8881 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC 8941 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT 9001 TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT 9061 TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA 9121 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 9181 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT 9241 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 9301 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 9361 CTTTTTTGGA GGCCTAGACT TTTGCAGAGA CGGCCCAAAT TCGTAATCAT GGTCATAGCT 9421 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 9481 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 9541 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 9601 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 9661 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 9721 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 9781 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 9841 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 9901 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 9961 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 10021 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 10081 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 10141 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 10201 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 10261 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 10321 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 10381 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 10441 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 10501 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 10561 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 10621 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 10681 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 10741 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 10801 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 10861 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 10921 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 10981 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 11041 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 11101 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 11161 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 11221 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 11281 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 11341 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 11401 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 11461 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 11521 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT 11581 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG 11641 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 11701 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 11761 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 11821 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC 11881 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA 11941 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 12001 GTCACGACGT TGTAAAACGA CGGCCAGTGC CAAGCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.