pLV3-U6-ZDHHC15-sgRNA3-Cas9-YP

pLV3-U6-ZDHHC15-sgRNA3-Cas9-YPet-Blast plasmid

PVT91551

pLV3-U6-ZDHHC15-sgRNA3-Cas9-YPet-Blast plasmid Information

Plasmid name: pLV3-U6-ZDHHC15(human)-sgRNA3-Cas9-YPet-Blast
Alias: TGTAAAGATAGACTTCCAGT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Yellow
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-ZDHHC15-sgRNA3-Cas9-YPet-Blast plasmid Description

pLV3-U6-ZDHHC15-sgRNA3-Cas9-YPet-Blast This is a plasmid suitable for gene editing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-ZDHHC15-sgRNA3-Cas9-YPet-Blast plasmid Sequence

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgTGT AAAGATAGAC TTCCAGTgtt 2881 ttagagctaG AAAtagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc 2941 accgagtcgg tgcTTTTTTg aattcgctag ctaggtcttg aaaggagtgg gaattggctc 3001 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3061 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3121 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3181 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagag 3241 cgctgccacc ATGGACAAGA AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG 3301 CTGGGCCGTG ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA 3361 CACCGACCGG CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA 3421 AACAGCCGAG GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA 3481 CCGGATCTGC TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT 3541 CTTCCACAGA CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC 3601 CATCTTCGGC AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA 3661 CCTGAGAAAG AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC 3721 CCTGGCCCAC ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA 3781 CAACAGCGAC GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA 3841 GGAAAACCCC ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG 3901 CAAGAGCAGA CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT 3961 GTTCGGAAAC CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA 4021 CCTGGCCGAG GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA 4081 CCTGCTGGCC CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC 4141 CGACGCCATC CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT 4201 GAGCGCCTCT ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC 4261 TCTCGTGCGG CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA 4321 CGGCTACGCC GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA 4381 GCCCATCCTG GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA 4441 CCTGCTGCGG AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG 4501 AGAGCTGCAC GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG 4561 GGAAAAGATC GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG 4621 GGGAAACAGC AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA 4681 CTTCGAGGAA GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA 4741 CTTCGATAAG AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA 4801 CTTCACCGTG TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC 4861 CGCCTTCCTG AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG 4921 GAAAGTGACC GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC 4981 CGTGGAAATC TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT 5041 GCTGAAAATT ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA 5101 AGATATCGTG CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA 5161 AACCTATGCC CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC 5221 CGGCTGGGGC AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA 5281 GACAATCCTG GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT 5341 CCACGACGAC AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG 5401 CGATAGCCTG CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT 5461 CCTGCAGACA GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA 5521 GAACATCGTG ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG 5581 CCGCGAGAGA ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA 5641 AGAACACCCC GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA 5701 GAATGGGCGG GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA 5761 TGTGGACCAT ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT 5821 GACCAGAAGC GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA 5881 GAAGATGAAG AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT 5941 CGACAATCTG ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT 6001 CAAGAGACAG CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC 6061 CCGGATGAAC ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC 6121 CCTGAAGTCC AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA 6181 GATCAACAAC TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT 6241 GATCAAAAAG TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA 6301 CGTGCGGAAG ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT 6361 CTTCTACAGC AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT 6421 CCGGAAGCGG CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG 6481 CCGGGATTTT GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA 6541 GACCGAGGTG CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA 6601 TAAGCTGATC GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC 6661 CACCGTGGCC TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT 6721 GAAGAGTGTG AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA 6781 TCCCATCGAC TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA 6841 GCTGCCTAAG TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC 6901 CGGCGAACTG CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA 6961 CCTGGCCAGC CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT 7021 GTTTGTGGAA CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC 7081 CAAGAGAGTG ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA 7141 CCGGGATAAG CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA 7201 TCTGGGAGCC CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC 7261 CAGCACCAAA GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA 7321 GACACGGATC GACCTGTCTC AGCTGGGAGG CGACAAGCGA CCTGCCGCCA CAAAGAAGGC 7381 TGGACAGGCT AAGAAGAAGA AAGATTACAA AGACGATGAC GATAAGGGAT CCGCCACCAA 7441 CTTCAGCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC CCCGGCCCCa tgtctaaagg 7501 tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg 7561 tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acgtacggta aattgacctt 7621 aaaattactc tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt 7681 aggttatggt gttcaatgtt ttgctagata cccagatcat atgaaacaac atgacttttt 7741 caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg 7801 taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga 7861 attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa 7921 ctataactct cacaatgttt acatcactgc tgacaaacaa aagaatggta tcaaagctaa 7981 cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa ttagctgacc attatcaaca 8041 aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatcctatca 8101 atctgcctta ttcaaagatc caaacgaaaa gagagaccac atggtcttgt tagaattttt 8161 gactgctgct ggtattaccg agggtatgaa tgaattgtac aaaGGCGCAA CAAACTTCTC 8221 TCTGCTGAAA CAAGCCGGAG ATGTCGAAGA GAATCCTGGA CCGATGGCCA AGCCTTTGTC 8281 TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC 8341 TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT 8401 CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC GTGGTGCTGG GCACTGCTGC 8461 TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT 8521 CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT CTGCATCCTG GGATCAAAGC 8581 CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC 8641 TGGTTATGTG TGGGAGGGCT AAGAATTCGA TATCAAGCTT ATCGGTAATC AACCTCTGGA 8701 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 8761 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 8821 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 8881 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 8941 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 9001 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 9061 TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC 9121 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 9181 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 9241 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCGTCGA CTTTAAGACC AATGACTTAC 9301 AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT 9361 CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG 9421 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 9481 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 9541 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA Gggcccgttt aaacccgctg 9601 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 9661 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 9721 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 9781 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 9841 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 9901 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 9961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 10021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 10081 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 10141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 10201 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 10261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 10321 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 10381 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 10441 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 10501 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 10561 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 10621 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 10681 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat 10741 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca 10801 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg 10861 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg 10921 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg 10981 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc 11041 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg 11101 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg 11161 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 11221 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 11281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 11341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 11401 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 11461 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 11521 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 11581 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 11641 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 11701 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 11761 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 11821 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 11881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 11941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 12001 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 12061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 12121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 12181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 12241 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 12301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 12361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 12421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 12481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 12541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 12601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 12661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 12721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 12781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 12841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 12901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 12961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 13021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 13081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 13141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 13201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 13261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 13321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 13381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 13441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 13501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 13561 ttccgcgcac atttccccga aaagtgccac ctgac

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.