pCMV-3×FLAG-FEM1B-Neo plasmid

pCMV-3×FLAG-FEM1B-Neo plasmid

PVT91402

pCMV-3×FLAG-FEM1B-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-FEM1B(human)-Neo
Alias: NM_015322.5;F1A-ALPHA; F1AA; FEM1-beta
Gene lenght: 1884bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-FEM1B-Neo plasmid Description

pCMV-3×FLAG-FEM1B-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-FEM1B-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggagggcc tggctggcta tgtatacaag 1021 gcggccagcg agggcaaggt gctgactctg gccgccttgc ttctcaaccg gtctgaaagc 1081 gacatccgct atctgcttgg ctatgtcagc cagcagggag ggcagcgctc cacgcccctc 1141 atcatcgcag cccgcaatgg acacgcaaag gtggtacgct tgctcttaga acattaccgg 1201 gtgcagactc agcagactgg caccgtccgc ttcgacgggt atgtcattga tggtgccact 1261 gctctttggt gtgcagctgg agcaggacat tttgaagttg ttaaacttct agtcagccat 1321 ggagccaacg tgaaccatac cacagtaact aattcaaccc ccctgcgggc agcatgcttt 1381 gatggcagac tggacattgt gaaatacttg gttgaaaata atgccaacat cagcattgcc 1441 aacaaatatg acaacacctg cctaatgatt gcggcatata agggacacac tgatgtggtc 1501 agataccttt tagaacaacg tgctgatccc aatgccaaag cacattgtgg agccacagca 1561 ttgcactttg cagctgaagc tgggcacata gatattgtga aagagctgat aaaatggcgt 1621 gctgctatag tagtgaatgg ccatgggatg acgccattga aagtagctgc cgaaagctgt 1681 aaagctgatg tcgtagaact gttactctct catgctgatt gcgaccgaag aagtcggatt 1741 gaagctttgg aactcttggg tgcctccttt gcaaatgacc gtgagaacta tgacatcata 1801 aagacatacc actatctata tttagccatg ttagagaggt tccaagatgg tgataacatt 1861 ctcgaaaaag aggttcttcc accaatccat gcttatggga atagaactga atgtagaaat 1921 cctcaggaac tggagtccat tcggcaagac agagatgctc ttcatatgga aggccttata 1981 gttcgggaac ggattttagg tgctgacaat attgatgttt ctcatcccat catttacaga 2041 ggagctgttt atgcggataa tatggaattt gagcagtgta tcaagttgtg gcttcatgcc 2101 ctgcacctca gacaaaaagg taacaggaac acccacaagg atcttcttcg atttgctcaa 2161 gttttctcac aaatgataca tttgaatgaa actgtgaagg ccccagacat agaatgtgtt 2221 ttgagatgca gtgttttgga aatagaacaa agtatgaaca gagtgaaaaa tatttcagat 2281 gctgatgtcc acaatgctat ggacaattat gaatgtaatc tctatacctt tctgtattta 2341 gtgtgcatct ctaccaaaac acagtgcagc gaagaagatc agtgcaaaat taacaagcag 2401 atctacaacc tgattcacct tgatcccaga actcgtgaag gtttcacctt gctgcatctg 2461 gctgtcaatt ccaatactcc agttgatgat ttccacacca atgacgtctg cagctttcca 2521 aatgcacttg tcacaaagct cctgctggac tgtggtgctg aggtgaatgc cgtggacaat 2581 gagggaaaca gtgcccttca tattatcgtt cagtacaaca ggcccatcag tgattttttg 2641 accttgcact ccatcatcat tagcctagtt gaagccggag ctcacactga catgacgaat 2701 aaacagaata agactccgct agacaaaagt acaactgggg tatctgaaat actgcttaaa 2761 actcaaatga agatgagtct caagtgcctg gctgcccgag cagttcgggc taatgacatt 2821 aactaccaag accagatccc cagaactctt gaagagtttg ttggatttca ttaagaattc 2881 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga 2941 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 3001 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 3061 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 3121 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 3181 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 3241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 3301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 3361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 3421 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 3481 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3541 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 3601 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 3661 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3721 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 3781 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3901 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3961 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 4021 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 4081 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 4141 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 4201 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 4261 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 4321 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 4381 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 4441 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 4501 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 4561 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 4621 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 4681 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 4741 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4801 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4861 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4921 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4981 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 5161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 5221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 5281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 5341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 5401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 5461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 5521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 5821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6001 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 6061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 6121 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 6181 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 6241 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6301 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6361 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6421 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6481 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 6541 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 6601 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 6661 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 6721 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 6781 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 6841 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 6901 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 6961 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7021 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7081 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7141 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 7201 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 7261 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7321 cacatttccc cgaaaagtgc cacctgacgt c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.