pCMV-mCherry-PPP1R3A-3×Myc-Neo

pCMV-mCherry-PPP1R3A-3×Myc-Neo plasmid

PVT91267

pCMV-mCherry-PPP1R3A-3×Myc-Neo plasmid Information

Plasmid name: pCMV-mCherry-PPP1R3A(human)-3×Myc-Neo
Alias: NM_002711.4;GM; PP1G; PPP1R3;
Gene lenght: 3366bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pCMV-mCherry-PPP1R3A-3×Myc-Neo plasmid Description

pCMV-mCherry-PPP1R3A-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-PPP1R3A-3×Myc-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tgGaGGTGGA GGTAGTGGAG GAGGCGGAAG Caagcttatg 1381 gagccttctg aagtacctag tcagattagc aaagataatt ttttagaagt tcctaattta 1441 tctgactctc tttgtgaaga tgaagaagtt actttccaac ctggtttctc ccctcaacca 1501 agtagacgag gttctgattc ttctgaagac atatacctgg ataccccatc ttcaggtact 1561 agaagagttt catttgctga ttcctttgga ttcaatcttg tgtctgttaa agaatttgat 1621 tgctgggaat taccgagtgc ttcaaccact tttgacttag ggacggacat tttccacaca 1681 gaagaatatg ttttagcccc actgtttgac ttgccttctt caaaagaaga tcttatgcaa 1741 caactccaaa tacagaaagc aatactggag tcaactgagt ctcttcttgg gtctacaagt 1801 atcaagggta ttattcgagt tttgaatgtt tcttttgaga agttagtata tgtaagaatg 1861 tctttagatg actggcagac acattatgac attttagcag aatatgttcc taattcatgt 1921 gatggtgaaa ctgaccagtt ctcctttaag attgtattgg ttcctcctta tcaaaaagat 1981 ggcagtaaag ttgagttttg tatacgttat gaaacttctg ttggtacatt ttggtcaaat 2041 aataatggca caaattatac attcatttgt caaaagaaag aacaagagcc ggagcctgta 2101 aaaccatgga aagaagttcc taacagacaa ataaaaggct gcttaaaggt aaaatcaagt 2161 aaagaagaat catcagtaac atcagaagaa aataactttg agaatccaaa gaatacagat 2221 acctatatcc caacaatcat ttgttctcat gaggacaagg aagatttgga agccagtaat 2281 cgaaatgtaa aagatgtaaa cagggaacat gatgaacata atgaaaaaga attagagttg 2341 atgataaatc aacacttaat aagaaccaga agtactgctt ccagagatga aaggaataca 2401 ttttcaacag atccagtcaa ttttccaaat aaagcagagg ggttagagaa gaagcaaatc 2461 catggtgaaa tatgtactga cttgttccaa aggtctctgt ctccaagttc atcagcagaa 2521 agctccgtaa agggagattt ttactgcaat gaaaaatatt cctcaggaga tgactgtaca 2581 catcaacctt cagaggaaac tacttcaaat atgggagaaa tcaagccatc attgggagat 2641 actagtagtg atgaactagt gcaattacat actggcagca aagaagtcct ggatgataat 2701 gctaatccag cccatggcaa tggcacagtg caaatacctt gcccctcttc agatcaacta 2761 atggcaggaa accttaataa aaaacatgaa ggaggagcta aaaatattga agtaaaagat 2821 ttgggatgtt tacgaagaga tttccattca gatacgtcgg catgtctcaa agaatcaaca 2881 gaagaaggat cttctaagga agattattat ggcaatggta aggatgatga agaacaaaga 2941 atatatttag gtgttaatga aaaacaaaga aaaaatttcc aaacaatctt acatgaccaa 3001 gaaaggaaga tgggtaaccc taaaataagt gtggcaggga ttggagctag taacagagac 3061 ctggctactc tgctgagcga acataccgca atccccaccc gggcaatcac agcagatgtg 3121 tctcattcac caaggacaaa tttaagttgg gaagaagctg tgttaacccc agagcatcat 3181 catttgacta gtgaaggcag cgctttagga gggataactg gtcaagtttg ttcatcaaga 3241 actggaaatg ttttgaggaa tgattatctt ttccaagttg aagaaaaatc aggtgggatt 3301 aattctgaag atcaggataa tagcccacag cataaacaaa gttggaatgt tctggaaagt 3361 cagggaaaat caagagagaa taagacaaac ataacagagc atatcaaagg acaaacagat 3421 tgtgaagacg tgtggggaaa aagagataat acgaggagtt tgaaagctac tacagaagaa 3481 ttgtttacct gccaagaaac agtgtgctgt gaactgtctt ctctagctga tcatggcatt 3541 actgagaaag cagaagctgg tacagcctat ataattaaga caacatcaga aagtactcca 3601 gaaagcatgt ctgctagaga aaaagcaata attgctaagc tacctcaaga gacagcacga 3661 agtgacaggc ccatcgaggt aaaggaaaca gcgtttgatc cacatgaagg gagaaatgat 3721 gattcacatt ataccctttg tcaacgagat acagtaggtg taatctatga caatgatttt 3781 gaaaaggaat cacgtttagg tatttgtaat gtacgtgtag atgaaatgga gaaggaagaa 3841 accatgtcta tgtacaatcc taggaagaca catgacaggg agaaatgtgg cactggaaat 3901 ataacatctg tggaagaatc ctcatgggtc attacagaat atcaaaaagc aacttcaaaa 3961 ctggatttac agttgggaat gttaccaaca gacaaaactg tattttcaga aaacagagat 4021 cttaggcagg ttcaagaatt atcaaagaaa acagactcgg atgccattgt gcattctgct 4081 tttaactcag acactaatag agctcctcag aatagctctc ctttttccaa acatcatact 4141 gaaatttcag tgtcaactaa tgagcaggca attgctgtag agaatgcagt tactaccatg 4201 gctagccaac ctatttctac gaaatcagaa aatatttgta attcaacaag agaaatccag 4261 ggtattgaga agcaccctta tcctgagtct aaacctgaag aagtttccag aagttcagga 4321 atagtgacat caggtagtag aaaagaaaga tgcataggcc agattttcca aacagaagag 4381 tatagtgtgg aaaaatctct agggccaatg attttaatca acaaacctct tgagaatatg 4441 gaagaagcaa ggcatgaaaa tgaaggatta gtaagctctg ggcaatcact atacacttca 4501 ggtgaaaagg aatctgacag ctctgcttct actagtcttc ctgttgagga aagtcaagct 4561 caaggcaacg aatctttgtt ttcaaaatat accaactcta aaatacctta tttccttttg 4621 tttctgatat ttcttataac tgtctaccat tatgacttaa tgattggctt gacattctac 4681 gttttgtcat tgtcctggct atcctgggaa gagggtagac aaaaagagtc tgtcaaaaag 4741 aaggaattcG AACAAAAACT AATATCAGAG GAAGATTTGG AACAGAAACT AATCAGTGAA 4801 GAAGATTTAG AGCAAAAGTT AATTTCCGAG GAGGACTTAT AGgatccacc ggatctagat 4861 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 4921 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 4981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa 5101 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 5161 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 5221 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 5281 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 5341 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 5401 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 5461 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 5521 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg 5581 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 5641 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga 5701 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 5761 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 5821 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 5881 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 5941 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 6001 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca 6061 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 6121 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 6181 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 6241 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 6301 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 6361 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 6421 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 6481 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 6541 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 6601 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 6661 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 6721 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 6781 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 6841 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 6901 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 6961 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 7021 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa 7081 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg 7141 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac 7201 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa 7261 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct 7321 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 7381 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 7441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 7501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 7561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 7621 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 7681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 7741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 7801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 7861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 7921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 7981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 8041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 8101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 8161 ataaccgtat taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.