pCMV-3×HA-KEAP1-EGFP-Neo plasm

pCMV-3×HA-KEAP1-EGFP-Neo plasmid

PVT91241

pCMV-3×HA-KEAP1-EGFP-Neo plasmid Information

Plasmid name: pCMV-3×HA-KEAP1(human)-EGFP-Neo
Alias: NM_203500.2;INrf2; KLHL19
Gene lenght: 1872bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-KEAP1-EGFP-Neo plasmid Description

pCMV-3×HA-KEAP1-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-KEAP1-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCATGT ACCCATACGA CGTACCTGAT TATGCAACTT 961 ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC TACGCAatgc 1021 agccagatcc caggcctagc ggggctgggg cctgctgccg attcctgccc ctgcagtcac 1081 agtgccctga gggggcaggg gacgcggtga tgtacgcctc cactgagtgc aaggcggagg 1141 tgacgccctc ccagcatggc aaccgcacct tcagctacac cctggaggat cataccaagc 1201 aggcctttgg catcatgaac gagctgcggc tcagccagca gctgtgtgac gtcacactgc 1261 aggtcaagta ccaggatgca ccggccgccc agttcatggc ccacaaggtg gtgctggcct 1321 catccagccc tgtcttcaag gccatgttca ccaacgggct gcgggagcag ggcatggagg 1381 tggtgtccat tgagggtatc caccccaagg tcatggagcg cctcattgaa ttcgcctaca 1441 cggcctccat ctccatgggc gagaagtgtg tcctccacgt catgaacggt gctgtcatgt 1501 accagatcga cagcgttgtc cgtgcctgca gtgacttcct ggtgcagcag ctggacccca 1561 gcaatgccat cggcatcgcc aacttcgctg agcagattgg ctgtgtggag ttgcaccagc 1621 gtgcccggga gtacatctac atgcattttg gggaggtggc caagcaagag gagttcttca 1681 acctgtccca ctgccaactg gtgaccctca tcagccggga cgacctgaac gtgcgctgcg 1741 agtccgaggt cttccacgcc tgcatcaact gggtcaagta cgactgcgaa cagcgacggt 1801 tctacgtcca ggcgctgctg cgggccgtgc gctgccactc gttgacgccg aacttcctgc 1861 agatgcagct gcagaagtgc gagatcctgc agtccgactc ccgctgcaag gactacctgg 1921 tcaagatctt cgaggagctc accctgcaca agcccacgca ggtgatgccc tgccgggcgc 1981 ccaaggtggg ccgcctgatc tacaccgcgg gcggctactt ccgacagtcg ctcagctacc 2041 tggaggctta caaccccagt gacggcacct ggctccggtt ggcggacctg caggtgccgc 2101 ggagcggcct ggccggctgc gtggtgggcg ggctgttgta cgccgtgggc ggcaggaaca 2161 actcgcccga cggcaacacc gactccagcg ccctggactg ttacaacccc atgaccaatc 2221 agtggtcgcc ctgcgccccc atgagcgtgc cccgtaaccg catcggggtg ggggtcatcg 2281 atggccacat ctatgccgtc ggcggctccc acggctgcat ccaccacaac agtgtggaga 2341 ggtatgagcc agagcgggat gagtggcact tggtggcccc aatgctgaca cgaaggatcg 2401 gggtgggcgt ggctgtcctc aatcgtctcc tttatgccgt ggggggcttt gacgggacaa 2461 accgccttaa ttcagctgag tgttactacc cagagaggaa cgagtggcga atgatcacag 2521 caatgaacac catccgaagc ggggcaggcg tctgcgtcct gcacaactgt atctatgctg 2581 ctgggggcta tgatggtcag gaccagctga acagcgtgga gcgctacgat gtggaaacag 2641 agacgtggac tttcgtagcc cccatgaagc accggcgaag tgccctgggg atcactgtcc 2701 accaggggag aatctacgtc cttggaggct atgatggtca cacgttcctg gacagtgtgg 2761 agtgttacga cccagataca gacacctgga gcgaggtgac ccgaatgaca tcgggccgga 2821 gtggggtggg cgtggctgtc accatggagc cctgccggaa gcagattgac cagcagaact 2881 gtacctgtGC ctcgagtggc ggtggaggaa gcggcggagg tggatctatg gtgagcaagg 2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagtct agagggcccg 3661 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3721 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3781 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3841 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3901 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4141 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4261 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4381 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4441 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4561 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4741 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4801 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4861 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4921 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4981 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 5041 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 5101 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 5161 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5221 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5281 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5341 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5401 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5461 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5521 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5581 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5881 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 5941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 6121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 6181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 6781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6841 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc 6901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 7021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 7081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.