pLV3-CMV-SMC5-EF1a-TagRFP-Puro

pLV3-CMV-SMC5-EF1a-TagRFP-Puro plasmid

PVT91191

pLV3-CMV-SMC5-EF1a-TagRFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SMC5(human)-EF1a-TagRFP-Puro
Alias: NM_015110.4;ATELS2; SMC5L1;
Gene lenght: 3306bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SMC5-EF1a-TagRFP-Puro plasmid Description

pLV3-CMV-SMC5-EF1a-TagRFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SMC5-EF1a-TagRFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catggcgact ccgagcaaga agacgtcaac tccaagcccc 2341 cagccttcca agagagctct cccgagagac ccttcgtcgg aggtcccgag caagaggaag 2401 aattcggccc cgcagctgcc gctgttgcag tcgtccgggc ctttcgtgga aggctctatc 2461 gtccgcatct cgatggagaa cttcctaaca tatgatattt gtgaagtatc tcctggaccc 2521 cacttgaata tgatcgttgg agccaatgga acagggaagt cgagcattgt gtgtgccatt 2581 tgccttggtt tagctggaaa acctgctttc atgggacgag cagataaggt tgggtttttt 2641 gtgaagagag gatgttctag aggcatggtt gaaattgaat tgttcagggc ttctggaaat 2701 cttgtaatca cccgtgagat tgatgtggca aaaaatcagt ccttttggtt catcaacaaa 2761 aaatctacaa cccagaaaat agtggaagag aaagttgcag ccttaaatat tcaagtgggg 2821 aatctttgcc agtttctccc tcaggacaaa gttggagaat ttgctaaact cagcaaaatt 2881 gaactcctcg aagccactga aaagtcaatt ggtcccccag aaatgcacaa atatcactgt 2941 gaactcaaaa acttaaggga gaaagaaaaa cagctcgaga cctcatgcaa agagaaaact 3001 gagtatctac agaaaatggt tcagaggaat gaaagatata aacaagatgt ggagaggttc 3061 tatgaacgga agcgacattt agatttaatt gagatgcttg aagcaaaaag gccatgggtg 3121 gaatatgaaa atgttcgtca ggaatatgaa gaagtaaaac tagttcgtga ccgagtgaag 3181 gaagaggtca gaaaacttaa agaagggcag attcctgtaa catgtcgaat tgaagaaatg 3241 gaaaacgagc gtcacaattt ggaggctcga atcaaagaaa aggcaacaga tattaaggag 3301 gcatctcaaa aatgcaaaca gaagcaagat gttatagaaa ggaaagataa acatattgag 3361 gaacttcagc aggctttaat agtaaagcaa aatgaagagc ttgaccgaca gaggagaata 3421 ggtaataccc gcaaaatgat agaggatttg caaaatgaac taaagaccac ggaaaactgc 3481 gagaatcttc agccccagat tgatgccatt acaaatgatc tgagacggat tcaggatgaa 3541 aaggcattat gtgaaggcga aataattgat aagcgaagag agagggaaac tctagagaag 3601 gagaaaaaga gtgtggacga tcatattgta cgttttgaca atcttatgaa tcagaaggaa 3661 gataagctaa gacagagatt ccgtgacacg tatgatgctg ttttatggct aagaaataac 3721 agagacaaat ttaaacaaag agtctgtgag cccataatgc tcacgatcaa tatgaaagat 3781 aataaaaatg ccaaatatat tgaaaatcat attccatcaa atgacttaag agcctttgta 3841 tttgaaagtc aagaagatat ggaggttttc ctcaaagagg ttcgtgacaa taaaaaatta 3901 agagtaaatg ctgttattgc tcccaagagt tcatatgcag acaaagcacc ttcaagatct 3961 ttgaatgaac ttaaacaata cggatttttc tcttatttga gagaattatt tgatgcacct 4021 gatcctgtaa tgagttacct ttgctgtcag tatcatattc atgaagttcc tgtaggaact 4081 gaaaagacca gagaaagaat tgaacgggta atacaagaaa cccgattaaa acagatttat 4141 acagcagaag aaaagtatgt ggtgaaaact tctttttatt caaacaaagt tatttctagt 4201 aacacatctc taaaagtagc gcagtttctc actgtcactg tggacctaga gcagagaaga 4261 cacttagaag aacagctaaa ggaaattcat agaaaattgc aagcagtgga ttcagggttg 4321 attgccttac gtgaaacaag caaacatctg gagcacaaag acaatgaact tagacaaaag 4381 aagaaggagc ttcttgagag aaaaaccaag aaaagacaac tggaacaaaa aatcagttcc 4441 aaactaggaa gtttaaagct gatggaacag gatacttgca atcttgaaga ggaagagcga 4501 aaagcaagta ccaaaatcaa agaaataaat gttcaaaaag cgaaacttgt taccgaatta 4561 acaaacctaa taaagatttg tacttctttg catatacaaa aagtagattt aattctccaa 4621 aatactacag tgatctctga gaagaacaaa ttagaatcag attatatggc cgcatcttca 4681 caactccgtc ttacagagca acatttcatt gaattggatg aaaatagaca gagattattg 4741 cagaaatgca aggaacttat gaaaagagct aggcaagtat gtaacctggg tgcagagcag 4801 actcttcctc aagaatacca gacacaagta cccaccattc caaatggaca caactcctca 4861 ctccccatgg ttttccaaga ccttccaaac acattggatg aaattgatgc tttattaact 4921 gaagaaagat caagagcttc ctgcttcacg ggactgaatc ctacaattgt tcaggaatat 4981 acaaaaagag aagaagaaat agaacagtta actgaggaac taaagggaaa gaaagttgaa 5041 ctagatcaat acagggaaaa catttcacag gtaaaagaaa ggtggcttaa tcctttaaaa 5101 gagctggtag aaaaaattaa tgaaaaattc agcaattttt ttagttccat gcagtgtgct 5161 ggtgaagttg atctccatac agaaaatgag gaagattatg ataaatatgg aattcgaatt 5221 agagtcaaat ttcgaagtag tactcaactg catgaattaa ctcctcatca tcaaagtgga 5281 ggtgaaagaa gtgtttctac catgttatac ttgatggcac ttcaggagct aaatagatgt 5341 ccattcagag tagttgatga aatcaatcag ggaatggacc caatcaatga acggagagtg 5401 tttgaaatgg ttgtaaatac tgcctgtaaa gaaaatacat ctcaatactt tttcataaca 5461 ccaaagctcc tgcaaaatct tccttattct gaaaagatga cagttttgtt tgtctacaat 5521 ggccctcata tgctggaacc aaacacatgg aatttaaagg ctttccaaag gcggcggcgc 5581 cgtattacat tcactcaacc ttcttaaGGA TCCGCGGCCG CGAAGGATCT GCGATCGCTC 5641 CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG 5701 GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT 5761 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT 5821 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG 5881 GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT 5941 GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT 6001 AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG 6061 ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC 6121 GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC 6181 CATGGTGTCT AAGGGCGAAG AGCTGATTAA GGAGAACATG CACATGAAGC TGTACATGGA 6241 GGGCACCGTG AACAACCACC ACTTCAAGTG CACATCCGAG GGCGAAGGCA AGCCCTACGA 6301 GGGCACCCAG ACCATGAGAA TCAAGGTGGT CGAGGGCGGC CCTCTCCCCT TCGCCTTCGA 6361 CATCCTGGCT ACCAGCTTCA TGTACGGCAG CAGAACCTTC ATCAACCACA CCCAGGGCAT 6421 CCCCGATTTC TTTAAGCAGT CCTTCCCTGA GGGCTTCACA TGGGAGAGAG TCACCACATA 6481 CGAAGACGGG GGCGTGCTGA CCGCTACCCA GGACACCAGC CTCCAGGACG GCTGCCTCAT 6541 CTACAACGTC AAGATCAGAG GGGTGAACTT CCCATCCAAC GGCCCTGTGA TGCAGAAGAA 6601 AACACTCGGC TGGGAGGCCA ACACCGAGAT GCTGTACCCC GCTGACGGCG GCCTGGAAGG 6661 CAGAACCGAC ATGGCCCTGA AGCTCGTGGG CGGGGGCCAC CTGATCTGCA ACTTCAAGAC 6721 CACATACAGA TCCAAGAAAC CCGCTAAGAA CCTCAAGATG CCCGGCGTCT ACTATGTGGA 6781 CCACAGACTG GAAAGAATCA AGGAGGCCGA CAAAGAGACC TACGTCGAGC AGCACGAGGT 6841 GGCTGTGGCC AGATACTGCG ACCTCCCTAG CAAACTGGGG CACAAACTTA ATGGCATGGA 6901 CGAGCTGTAC AAGgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc 6961 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 7021 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 7081 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 7141 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 7201 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 7261 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 7321 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 7381 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 7441 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 7501 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 7561 tgcctgaAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 7621 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 7681 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 7741 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 7801 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 7861 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 7921 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG 7981 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 8041 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 8101 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG 8161 TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA 8221 AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA 8281 CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC 8341 CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT 8401 TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA 8461 GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT 8521 CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 8581 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 8641 TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC 8701 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 8761 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG 8821 CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 8881 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 8941 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 9001 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 9061 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 9121 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 9181 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 9241 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 9301 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 9361 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 9421 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 9481 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 9541 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 9601 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 9661 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 9721 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 9781 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 9841 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 9901 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 9961 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 10021 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 10081 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 10141 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 10201 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 10261 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 10321 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 10381 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 10441 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 10501 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 10561 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 10621 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 10681 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 10741 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 10801 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 10861 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 10921 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 10981 TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA 11041 AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 11101 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 11161 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 11221 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 11281 CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA 11341 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC 11401 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC 11461 CAGTGCCAAG CTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.