pCMV-STAT3-mVenus-Neo plasmid

pCMV-STAT3-mVenus-Neo plasmid

PVT91183

pCMV-STAT3-mVenus-Neo plasmid Information

Plasmid name: pCMV-STAT3(dog)-mVenus-Neo
Alias: XM_038676442.1
Gene lenght: 2310bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Canine
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-STAT3-mVenus-Neo plasmid Description

pCMV-STAT3-mVenus-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-STAT3-mVenus-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCatggcc caatggaatc agctacagca actcgacaca 661 cggtacttag agcagcttca tcagctgtac agcgacagct tcccaatgga gctacggcag 721 tttctggccc cgtggattga gagtcaagat tgggcatatg cagccagcaa agaatcacat 781 gctactttgg tgtttcataa tctcttgggt gagattgacc agcagtacag ccgtttccta 841 caggagtcaa atgttctcta tcagcacaat ctgcgaagaa tcaagcagtt ccttcagagc 901 agatatctgg agaagccaat ggagattgcc cgaattgtgg cccggtgctt gtgggaagag 961 tcccgcctgc tacaaactgc agccactgca gctcagcaag ggggccaggc cagccacccc 1021 acagccgccg tggtgacgga gaagcaacag atgctggagc agcatcttca agatgttcgg 1081 aagcgagtac aggatctaga acagaaaatg aaagtggtcg agaatctcca ggatgacttt 1141 gatttcaact acaaaaccct caagagtcaa ggagacatgc aggatctgaa tggaaacaac 1201 cagtcggtga ccagacagaa gatgcagcag ctggagcaga tgctcactgc actggaccag 1261 atgcggcgga gcatcgtgag tgagttagcg gggctgttgt caactatgga gtacgtgcag 1321 aaaactctca cggatgaaga gctggctgat tggaagaggc ggcaacagat tgcgtgcatt 1381 ggaggccccc ccaacatctg cctagatcgc ctagaaaact ggataacctc attagcagag 1441 tcacaacttc agacccgcca acaaattaag aaactggagg agttacaaca aaaagtgtcc 1501 tataaagggg atcccatcgt ccagcaccgg ccaatgctag aggagaggat tgtagagctg 1561 tttaggaact taatgaaaag tgcctttgtg gttgagcggc agccttgcat gcccatgcat 1621 cctgaccggc cgttagtcat caagactggc gtccagttca ctaccaaagt cagattgctg 1681 gtcaaattcc cggagttaaa ttatcagctt aaaattaaag tgtgcattga caaagactct 1741 ggggatgtcg cagctctcag aggatctcgg aaatttaaca ttctgggcac aaacacaaaa 1801 gtgatgaaca tggaagagtc caacaacggc agcctgtctg cagaattcaa acacctgacc 1861 ctgagagagc aaagatgtgg taatgggggc cgagccaact gtgatgcctc cctgattgtc 1921 actgaggagc tgcacctgat cacctttgag acggaggtgt atcaccaagg cctcaagatt 1981 gaccttgaga cccactcctt gccagttgtg gtgatctcga atatctgtca gatgccaaat 2041 gcctgggcat caatcctgtg gtacaacatg ctgaccagca accccaagaa cgtgaacttt 2101 ttcaccaagc ccccgattgg aacctgggat caagtggctg aggtgctgag ctggcagttc 2161 tcctccacca caaagcgagg actgagcatc gagcagttga ctacgctggc agagaaactc 2221 ttaggacctg gtgtgaacta ctcagggtgt cagattacgt gggctaaatt ttgcaaagaa 2281 aacatggctg gcaaaggctt ctccttctgg gtttggctag acaatatcat tgacctcgtg 2341 aaaaagtata tcctggctct ttggaatgaa ggatacataa tgggttttat cagtaaggag 2401 cgggagcggg ccatcctgag cacaaagccc ccaggcacct tcctgctacg gttcagtgaa 2461 agcagcaaag aaggaggtgt caccttcact tgggtggaga aggacatcag cggtaagacc 2521 cagatccagt cggtggaacc atataccaag caacagctga acaacatgtc gtttgctgaa 2581 atcatcatgg gctataagat catggatgct accaacatcc tggtgtctcc actggtctat 2641 ctctaccccg acattcctaa ggaagaggca tttggaaagt actgtcgacc agagagccag 2701 gaacatcctg aagccgaccc aggtagtgct gccccatacc tgaagaccaa gtttatctgt 2761 gtgacaccaa cgacgtgcag caataccatt gacctgccga tgtccccccg cactttagat 2821 tcattgatgc agtttggaaa taatggtgaa ggtgctgagc cctcagcagg agggcagttc 2881 gagaccctca cgttcgacat ggagttgacc tcggagtgcg ctacctcccc gatgGCggat 2941 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 3001 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 3061 ggcgatgcca cctacggcaa gctgaccctg aagctcatct gcaccaccgg caagctgccc 3121 gtgccctggc ccaccctcgt gaccaccctc ggctacggcc tgcagtgctt cgcccgctac 3181 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 3241 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 3301 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 3361 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc 3421 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc 3481 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 3541 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag 3601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 3661 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag 3721 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 3781 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 3841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 3901 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 3961 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 4021 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 4081 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 4141 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 4201 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 4261 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 4321 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 4381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 4441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 4501 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4621 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4681 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4861 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4921 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4981 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 5041 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 5101 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 5161 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 5221 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 5281 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 5341 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 5401 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 5461 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 5521 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 5581 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 5641 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 5701 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 5761 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 5821 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 5881 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 5941 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 6001 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 6061 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 6121 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 6181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6421 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.