pCMV-EGFP-EPHA8-Puro plasmid

pCMV-EGFP-EPHA8-Puro plasmid

PVT91098

pCMV-EGFP-EPHA8-Puro plasmid Information

Plasmid name: pCMV-EGFP-EPHA8(human)-Puro
Alias: NM_020526.5；EEK; EK3; HEK3
Gene lenght: 3018bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-EPHA8-Puro plasmid Description

pCMV-EGFP-EPHA8-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-EPHA8-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag AAGCTTgGCG GCGGAGGGAG TGGAGGAGGG 1621 GGAAGCatgg cccccgcccg gggccgcctg ccccctgcgc tctgggtcgt cacggccgcg 1681 gcggcggcgg ccacctgcgt gtccgcggcg cgcggcgaag tgaatttgct ggacacgtcg 1741 accatccacg gggactgggg ctggctcacg tatccggctc atgggtggga ctccatcaac 1801 gaggtggacg agtccttcca gcccatccac acgtaccagg tttgcaacgt catgagcccc 1861 aaccagaaca actggctgcg cacgagctgg gtcccccgag acggcgcccg gcgcgtctat 1921 gctgagatca agtttaccct gcgcgactgc aacagcatgc ctggtgtgct gggcacctgc 1981 aaggagacct tcaacctcta ctacctggag tcggaccgcg acctgggggc cagcacacaa 2041 gaaagccagt tcctcaaaat cgacaccatt gcggccgacg agagcttcac aggtgccgac 2101 cttggtgtgc ggcgtctcaa gctcaacacg gaggtgcgca gtgtgggtcc cctcagcaag 2161 cgcggcttct acctggcctt ccaggacata ggtgcctgcc tggccatcct ctctctccgc 2221 atctactata agaagtgccc tgccatggtg cgcaatctgg ctgccttctc ggaggcagtg 2281 acgggggccg actcgtcctc actggtggag gtgaggggcc agtgcgtgcg gcactcagag 2341 gagcgggaca cacccaagat gtactgcagc gcggagggcg agtggctcgt gcccatcggc 2401 aaatgcgtgt gcagtgccgg ctacgaggag cggcgggatg cctgtgtggc ctgtgagctg 2461 ggcttctaca agtcagcccc tggggaccag ctgtgtgccc gctgccctcc ccacagccac 2521 tccgcagctc cagccgccca agcctgccac tgtgacctca gctactaccg tgcagccctg 2581 gacccgccgt cctcagcctg cacccggcca ccctcggcac cagtgaacct gatctccagt 2641 gtgaatggga catcagtgac tctggagtgg gcccctcccc tggacccagg tggccgcagt 2701 gacatcacct acaatgccgt gtgccgccgc tgcccctggg cactgagccg ctgcgaggca 2761 tgtgggagcg gcacccgctt tgtgccccag cagacaagcc tggtgcaggc cagcctgctg 2821 gtggccaacc tgctggccca catgaactac tccttctgga tcgaggccgt caatggcgtg 2881 tccgacctga gccccgagcc ccgccgggcc gctgtggtca acatcaccac gaaccaggca 2941 gccccgtccc aggtggtggt gatccgtcaa gagcgggcgg ggcagaccag cgtctcgctg 3001 ctgtggcagg agcccgagca gccgaacggc atcatcctgg agtatgagat caagtactac 3061 gagaaggaca aggagatgca gagctactcc accctcaagg ccgtcaccac cagagccacc 3121 gtctccggcc tcaagccggg cacccgctac gtgttccagg tccgagcccg cacctcagca 3181 ggctgtggcc gcttcagcca ggccatggag gtggagaccg ggaaaccccg gccccgctat 3241 gacaccagga ccattgtctg gatctgcctg acgctcatca cgggcctggt ggtgcttctg 3301 ctcctgctca tctgcaagaa gaggcactgt ggctacagca aggccttcca ggactcggac 3361 gaggagaaga tgcactatca gaatggacag gcacccccac ctgtcttcct gcctctgcat 3421 caccccccgg gaaagctccc agagccccag ttctatgcgg aaccccacac ctacgaggag 3481 ccaggccggg cgggccgcag tttcactcgg gagatcgagg cctctaggat ccacatcgag 3541 aaaatcatcg gctctggaga ctccggggaa gtctgctacg ggaggctgcg ggtgccaggg 3601 cagcgggatg tgcccgtggc catcaaggcc ctcaaagccg gctacacgga gagacagagg 3661 cgggacttcc tgagcgaggc gtccatcatg gggcaattcg accatcccaa catcatccgc 3721 ctcgagggtg tcgtcacccg tggccgcctg gcaatgattg tgactgagta catggagaac 3781 ggctctctgg acaccttcct gaggacccac gacgggcagt tcaccatcat gcagctggtg 3841 ggcatgctga gaggagtggg tgccggcatg cgctacctct cagacctggg ctatgtccac 3901 cgagacctgg ccgcccgcaa cgtcctggtt gacagcaacc tggtctgcaa ggtgtctgac 3961 ttcgggctct cacgggtgct ggaggacgac ccggatgctg cctacaccac cacgggcggg 4021 aagatcccca tccgctggac ggccccagag gccatcgcct tccgcacctt ctcctcggcc 4081 agcgacgtgt ggagcttcgg cgtggtcatg tgggaggtgc tggcctatgg ggagcggccc 4141 tactggaaca tgaccaaccg ggatgtcatc agctctgtgg aggaggggta ccgcctgccc 4201 gcacccatgg gctgccccca cgccctgcac cagctcatgc tcgactgttg gcacaaggac 4261 cgggcgcagc ggcctcgctt ctcccagatt gtcagtgtcc tcgatgcgct catccgcagc 4321 cctgagagtc tcagggccac cgccacagtc agcaggtgcc caccccctgc cttcgtccgg 4381 agctgctttg acctccgagg gggcagcggt ggcggtgggg gcctcaccgt gggggactgg 4441 ctggactcca tccgcatggg ccggtaccga gaccacttcg ctgcgggcgg atactcctct 4501 ctgggcatgg tgctacgcat gaacgcccag gacgtgcgcg ccctgggcat caccctcatg 4561 ggccaccaga agaagatcct gggcagcatt cagaccatgc gggcccagct gaccagcacc 4621 caggggcccc gccggcacct ctgaGAATTC TGCAGATATC CAGCACAGTG GCGGCCGCTC 4681 GAGTCTAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG 4741 CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT 4801 GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT 4861 CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT 4921 GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG 4981 GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 5041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 5101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 5161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 5221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 5281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 5341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 5401 CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC 5461 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 5521 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT 5581 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG 5641 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT 5701 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 5761 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 5821 CGTTTCGCat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc 5881 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 5941 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 6001 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 6061 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 6121 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 6181 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 6241 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 6301 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 6361 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 6421 gtgcctgaGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC 6481 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 6541 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC 6601 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 6661 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 6721 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT 6781 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 6841 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 6901 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 6961 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 7021 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 7081 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 7141 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 7201 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 7261 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 7321 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 7381 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 7441 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 7501 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 7561 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 7621 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 7681 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 7741 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 7801 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 7861 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 7921 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 7981 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 8041 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 8101 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 8161 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 8221 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 8281 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 8341 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 8401 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 8461 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 8521 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 8581 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 8641 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 8701 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 8761 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 8821 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 8881 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA 8941 TCTCCCGATC CCCTATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.