pLV3-H1-2O2-MDM2-shRNA3-TetR-H

pLV3-H1-2O2-MDM2-shRNA3-TetR-Hyg plasmid

PVT90961

pLV3-H1-2O2-MDM2-shRNA3-TetR-Hyg plasmid Information

Plasmid name: pLV3-H1-2O2-MDM2(human)-shRNA3-TetR-Hyg
Alias: CTCTCGACTCAGAAGATTATA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,Tetracycline induction
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: H1-2O2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: H1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV3-H1-2O2-MDM2-shRNA3-TetR-Hyg plasmid Description

pLV3-H1-2O2-MDM2-shRNA3-TetR-Hyg This is a plasmid suitable for interference silencing in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV3-H1-2O2-MDM2-shRNA3-TetR-Hyg plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt 541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt 601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct 661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa 721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt 781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat 841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat 901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc 961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag 1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc 1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 2401 ggatccaccg gagcttacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt 2461 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc 2521 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc 2581 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat 2641 tccggaagtg cttgacattg gggagttcag cgagagcctg acctattgca tctcccgccg 2701 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc 2761 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg 2821 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat 2881 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt 2941 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct 3001 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt 3061 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt 3121 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc 3181 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact 3241 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga 3301 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc 3361 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag 3421 cactcgtccg agggcaaagg aatagggtac ctttaagacc aatgacttac aaggcagctg 3481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 3541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 3601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 3661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 3721 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 3781 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 3841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 3961 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4021 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4081 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc 4141 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 4201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 4261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 4321 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 4381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 4441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 4501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 4561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 4621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 4681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 4741 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 4801 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 4861 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 4921 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 4981 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 5041 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 5101 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 5161 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 5221 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 5281 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 5341 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 5401 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 5461 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 5521 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 5581 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 5641 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 5701 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 5761 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 5821 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 5881 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 5941 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6001 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 6061 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6121 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6181 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 6241 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 6301 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 6361 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 6421 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 6481 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 6541 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 6601 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 6661 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 6721 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 6781 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 6841 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 6901 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc 6961 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt 7021 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca 7081 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca 7141 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt 7201 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga 7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7381 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg 7441 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc 7501 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga 7561 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt 7621 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg 7681 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa 7741 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat 7801 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa 7861 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc 7921 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa 7981 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt 8041 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc 8101 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt 8161 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct 8221 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag 8281 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac 8341 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca 8401 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt 8461 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa 8521 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt 8581 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt 8641 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag 8701 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc 8761 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc 8821 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt 8881 gatagagact tataagttcc ctatcagtga tagagacacc ggCTCTCGAC TCAGAAGATT 8941 ATAttcaaga gaTATAATCT TCTGAGTCGA GAGTTTTTTg aattctcgac ctcgagacaa 9001 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 9061 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 9121 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg 9181 ctcgaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.