pDC316-ZsGreen1-U6-Tpm1-shRNA2

pDC316-ZsGreen1-U6-Tpm1-shRNA2 plasmid

PVT90849

pDC316-ZsGreen1-U6-Tpm1-shRNA2 plasmid Information

Plasmid name: pDC316-ZsGreen1-U6-Tpm1(rat)-shRNA2
Alias: CTGAAGATGAACTGGACAAAT
Gene lenght:
Host: Mammalian cells,Adenovirus
Use(s): Interference silence
Fragment type: shRNA
Species: Rat
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pDC316-ZsGreen1-U6-Tpm1-shRNA2 plasmid Description

pDC316-ZsGreen1-U6-Tpm1-shRNA2 This is a plasmid suitable for interference silencing in mammalian cells, and can also be used for adenovirus packaging.

pDC316-ZsGreen1-U6-Tpm1-shRNA2 plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggccca gtccaagcac 1021 ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag 1081 ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg 1141 tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg 1201 tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc 1261 tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc 1321 tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc 1381 tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag 1441 ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc 1501 atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag 1561 gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc 1621 gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc 1681 ggctccgcct tgccctaaag atctcgagat ccaccggatc tagataactg atcataatca 1741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 1801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 1861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 1921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtctgggca ggaagagggc 1981 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt 2041 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa 2101 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt 2161 accgtaactt gaaagtattt cgatttcttg ggtttatata tcttgtggaa aggacgaaac 2221 acctgcagCT GAAGATGAAC TGGACAAATt tcaagagaAT TTGTCCAGTT CATCTTCAGt 2281 tttttggatc ccaatgtcga cttcgagcaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgtctagc atcgaagatc 2461 caataacttc gtatagcata cattatacga agttataagt agcttggcgt aatcatggtc 2521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 2581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 2641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 2701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 3121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3361 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 3661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 3721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 3781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 4021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4261 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag 4321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 4381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 4441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 4501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 4561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 4621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 4681 aaccattatt atcatgacat taacctataa aaataggcgt atcactctag gcaaaatagc 4741 accctcccgc tccagaacaa catacagcgc ttcacagcgg cagcctaaca gtcagcctta 4801 ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac acggcaccag ctcaatcagt 4861 cacagtgtaa aaaagggcca agtgcagagc gagtatatat aggactaaaa aatgacgtaa 4921 cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc cagaaacgaa 4981 agccaaaaaa cccacaactt cctcaaatcg tcacttccgt tttcccacgt tacgtaactt 5041 cccattttaa gaaaactaca attcccaaca catacaagtt actccgccct aaaacctacg 5101 tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct cattatcata 5161 ttggcttcaa tccaaaataa ggtata

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.