pLV3-CMV-LINC02285-lncRNA-EF1-

pLV3-CMV-LINC02285-lncRNA-EF1-CopGFP-Puro plasmid

PVT90662

pLV3-CMV-LINC02285-lncRNA-EF1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LINC02285(human)-lncRNA-EF1-CopGFP-Puro
Alias: NR_146490.1
Gene lenght: 946bp
Host: Mammalian cells,lentivirus
Use(s): RNATranscription
Fragment type: ncRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LINC02285-lncRNA-EF1-CopGFP-Puro plasmid Description

pLV3-CMV-LINC02285-lncRNA-EF1-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-LINC02285-lncRNA-EF1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattccttt ttgagaagaa gcttgtgcct gtgcctggaa gcaaggaggg 2341 ctggaactgg ggccctgctg ggaagccctg tatctgcaga actgtgtccc gtgggggcca 2401 ggggacacag atttttgcag ccctctgatg ctgaagcctt tctggggctg ggatgaccca 2461 atgcccacct ggaccgtgca cttcctggtg accctctgag acaacttcct gggcagagtt 2521 gatcatcatg gagtcagtgc aagtctggga acagtggcct gaaactgcgc agctccagtg 2581 tgtcggtgga ggcccttcag tgaggagtcc cccagtctgg gtggagagcc agcccgatcg 2641 gcttgattcc tccactcttg acaccgtgag cctcagattc tgagagcttg ggaggatgga 2701 cagagactgc ggtcagcagg agaatgggaa gcagatggga gaggaagaag cttgcctttg 2761 gtggggacca ggaggctggg agcccaggcc cattcactgt aacatgtgca gtaaatccca 2821 ttatattcat gcaaagaatt ctacacagca aggaaaagga aagagctgtt ggcatgccac 2881 aacacaggtg gatctcacag acctactgct gagctaaaga aggcaaccac gcacatgtac 2941 atgctgcatg gggccgtttc tatctgccca gaaccaggca agaaccatct atgccaacag 3001 cttcattgct gctgtttctg gggttgttga ctggaggggc gaggggaccc tctcggatgt 3061 ggaaatgttc tgtgatgaga tagaggtttc ctgcatgtct gtgcctgtga gatgcatcca 3121 gctgcacact tcagatgcgt gcattttatt gcatatgtta tgccttgatt tttaaaagtc 3181 tttaaatatt taatcctgaa catccctacg aaacaaataa taaaacagcc ctaggctcct 3241 gagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3301 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3361 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3421 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3481 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3541 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 3601 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 3661 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 3721 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 3781 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 3841 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 3901 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 3961 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4021 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4081 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4141 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4201 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4261 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4321 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4381 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4441 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4501 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 4561 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 4621 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 4681 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 4741 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 4801 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 4861 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 4921 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 4981 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5041 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5101 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5161 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 5581 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 5641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 5701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 5761 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 5821 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 5881 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 5941 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6001 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6061 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6121 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6181 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 6301 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 6361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 6481 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6601 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6661 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7321 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 7981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8641 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 8701 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 8761 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 8821 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 8881 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 8941 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9001 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9061 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9121 agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.