pLV3-CMV-3×HA-CHEK1-EF1a-mCher

pLV3-CMV-3×HA-CHEK1-EF1a-mCherry-Puro plasmid

PVT90503

pLV3-CMV-3×HA-CHEK1-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-CHEK1(human)-EF1a-mCherry-Puro
Alias: NM_001114122.3;CHK1; OZEMA21;
Gene lenght: 1431bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-CHEK1-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-3×HA-CHEK1-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×HA-CHEK1-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgTACCC ATACGACGTA CCTGATTATG CAACTTATCC 2341 TTATGATGTA CCTGATTATG CTAGtTATCC TTATGATGTC CCAGACTACG CActcgagat 2401 ggcagtgccc tttgtggaag actgggactt ggtgcaaacc ctgggagaag gtgcctatgg 2461 agaagttcaa cttgctgtga atagagtaac tgaagaagca gtcgcagtga agattgtaga 2521 tatgaagcgt gccgtagact gtccagaaaa tattaagaaa gagatctgta tcaataaaat 2581 gctaaatcat gaaaatgtag taaaattcta tggtcacagg agagaaggca atatccaata 2641 tttatttctg gagtactgta gtggaggaga gctttttgac agaatagagc cagacatagg 2701 catgcctgaa ccagatgctc agagattctt ccatcaactc atggcagggg tggtttatct 2761 gcatggtatt ggaataactc acagggatat taaaccagaa aatcttctgt tggatgaaag 2821 ggataacctc aaaatctcag actttggctt ggcaacagta tttcggtata ataatcgtga 2881 gcgtttgttg aacaagatgt gtggtacttt accatatgtt gctccagaac ttctgaagag 2941 aagagaattt catgcagaac cagttgatgt ttggtcctgt ggaatagtac ttactgcaat 3001 gctcgctgga gaattgccat gggaccaacc cagtgacagc tgtcaggagt attctgactg 3061 gaaagaaaaa aaaacatacc tcaacccttg gaaaaaaatc gattctgctc ctctagctct 3121 gctgcataaa atcttagttg agaatccatc agcaagaatt accattccag acatcaaaaa 3181 agatagatgg tacaacaaac ccctcaagaa aggggcaaaa aggccccgag tcacttcagg 3241 tggtgtgtca gagtctccca gtggattttc taagcacatt caatccaatt tggacttctc 3301 tccagtaaac agtgcttcta gtgaagaaaa tgtgaagtac tccagttctc agccagaacc 3361 ccgcacaggt ctttccttat gggataccag cccctcatac attgataaat tggtacaagg 3421 gatcagcttt tcccagccca catgtcctga tcatatgctt ttgaatagtc agttacttgg 3481 caccccagga tcctcacaga acccctggca gcggttggtc aaaagaatga cacgattctt 3541 taccaaattg gatgcagaca aatcttatca atgcctgaaa gagacttgtg agaagttggg 3601 ctatcaatgg aagaaaagtt gtatgaatca ggttactata tcaacaactg ataggagaaa 3661 caataaactc attttcaaag tgaatttgtt agaaatggat gataaaatat tggttgactt 3721 ccggctttct aagggtgatg gattggagtt caagagacac ttcctgaaga ttaaagggaa 3781 gctgattgat attgtgagca gccagaagat ttggcttcct gccacatgag gatccgcggc 3841 cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 3901 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg 3961 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 4021 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 4081 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct 4141 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct 4201 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg 4261 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg 4321 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac 4381 cggcgcctac gctagacgcc accatggtga gcaagggcga ggaggataac atggccatca 4441 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 4501 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 4561 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 4621 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 4681 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 4741 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 4801 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 4861 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 4921 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 4981 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 5041 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 5101 agctgtacaa gggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg 5161 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 5221 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 5281 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 5341 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 5401 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 5461 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 5521 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 5581 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 5641 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 5701 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 5761 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5821 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5881 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5941 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 6001 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 6061 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 6121 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 6181 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6241 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6301 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc 6361 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6421 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 6481 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6541 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6601 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6661 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6721 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6841 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 6901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 6961 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 7021 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 7081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 9001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9241 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9421 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9481 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9541 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9601 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9661 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.