pCMV-Spi1-P2A-Batf3-T2A-Irf8-T

pCMV-Spi1-P2A-Batf3-T2A-Irf8-T2A-EGFP-Puro plasmid

PVT90424

pCMV-Spi1-P2A-Batf3-T2A-Irf8-T2A-EGFP-Puro plasmid Information

Plasmid name: pCMV-Spi1(mouse)-P2A-Batf3(mouse)-T2A-Irf8(mouse)-T2A-EGFP-Puro
Alias: NM_001378898.1;Dis-1; Dis1; PU.1; Sfpi-1; Sfpi1; Spi-1;Tcfpu1; Tfpu.1;NM_030060.2;9130211I03Rik; Snft;NM_001301811.1;ICSBP; Icsbp1; IRF-8; Myls;
Gene lenght: 846;354;1275;bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-Spi1-P2A-Batf3-T2A-Irf8-T2A-EGFP-Puro plasmid Description

pCMV-Spi1-P2A-Batf3-T2A-Irf8-T2A-EGFP-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-Spi1-P2A-Batf3-T2A-Irf8-T2A-EGFP-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT 901 TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCGC CACCatgtta 961 caggcgtgca aaatggaagg gttttccctc accgcccctc tcaggctcga cacctttctc 1021 tctcggcagc catcggatga cttggttact tacgattcag agctatacca acgtccaatg 1081 catgactact actccttcgt gggcagcgat ggagaaagcc atagcgatca ctactgggat 1141 ttctccgcac accatgtcca caacaacgag tttgagaact tccctgagaa ccacttcaca 1201 gagctgcaga gtgtgcagcc cccgcagcta cagcagctct atcgccacat ggagctggaa 1261 cagatgcacg tcctcgatac tcccatggtg ccaccccaca ccggcctcag tcaccaggtt 1321 tcctacatgc cccggatgtg cttcccttat caaaccttgt ccccagccca ccagcagagc 1381 tcagatgagg aggagggtga gaggcagagc cctcccctgg aggtgtctga tggagaagct 1441 gatggcttgg agcctgggcc aggtcttctg cacggggaga caggcagcaa gaaaaagatt 1501 cgcctgtacc agttcctgct ggacctgctg cgcagcggcg acatgaagga cagcatctgg 1561 tgggtggaca aggacaaagg taccttccag ttctcgtcca agcacaagga ggcgctggcg 1621 caccgctggg gcatccagaa gggcaaccgc aagaagatga cctaccagaa gatggcgcgc 1681 gcgctgcgca actacggcaa gacaggcgag gtgaagaaag tcaagaagaa gctcacctac 1741 cagttcagcg gcgaggtgct gggccgtggg ggcctggccg agcggcgcct cccgccccac 1801 GGCAGCGGCG CCACCAACTT CAGCCTGCTG AAGCAGGCCG GCGACGTGGA GGAGAACCCC 1861 GGCCCCGATA TCatgtcgca agggcccccc gcggtcagcg tgctgcagag aagcgtggat 1921 gcgcccggga accagccgca gagccccaag gacgatgaca ggaaagttcg aaggagagag 1981 aaaaaccggg ttgcagctca gaggagccgg aagaagcaga cccagaaggc tgacaagctc 2041 cacgaggagc acgagagcct ggagcaggag aactctgtgc tgcgcaggga gatttcgaag 2101 ctgaaggagg agctgcgtca cctgagcgag gtgctgaagg agcacgagaa gatgtgcccg 2161 ctgctgctgt gtcctatgaa ctttgtgcag cttcggtcag accccgtggc cagctgtcta 2221 ccacgagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 2281 GGCTCAGGTa tgtgtgaccg gaacggcggg cggcggctgc ggcagtggct gatcgaacag 2341 atcgacagca gcatgtaccc ggggctgatc tgggaaaatg atgagaagac catgttccgt 2401 atcccctgga agcatgccgg caagcaggat tacaatcagg aggtggatgc ttccatcttc 2461 aaggcctggg cagtttttaa agggaagttt aaagagggag acaaagctga accagccacg 2521 tggaagacga ggttacgctg tgctctgaac aagagcccag attttgaaga agtgactgac 2581 cggtcccagc tggacatttc tgagccatat aaagtttacc gaattgtccc cgaggaagaa 2641 caaaaatgca agctgggcgt ggcacctgca ggctgcatga gcgaagttcc tgagatggag 2701 tgtggccgct cagagattga ggagctgatc aaggaacctt ctgtggatga gtacatgggt 2761 atgaccaaga ggagcccatc cccaccagag gcctgcagga gccagatcct ccctgactgg 2821 tgggtccagc agcccagtgc aggcctgcca ctggtgaccg gatatgccgc ctatgacaca 2881 caccattcag ctttctccca gatggtcatc agcttctact acgggggcaa gctggtgggc 2941 caggccacca ccacctgcct tgaaggctgc cgtctctccc tgagccagcc ggggctgcct 3001 aagttgtatg ggccggatgg cctggaaccc gtgtgctttc cgacggccga caccatcccc 3061 agtgagcggc agaggcaggt gacccggaag ctgtttgggc acctggaacg tggcgtgcta 3121 ctgcacagca accgcaaggg cgtgttcgtg aagcggctgt gccagggccg cgtgttctgc 3181 agcggcaacg cggtggtgtg caagggcagg cccaacaagc tggagcggga cgaggtggtg 3241 caggtctttg acaccaacca gttcatccga gagctgcagc aattctacgc cacccagagc 3301 cgcctacctg acagcagggt ggtcctgtgc ttcggggagg agtttccgga cactgtgccc 3361 ttgcgctcca aactcattct ggtgcaggta gagcagctgt atgccaggca gctggtggag 3421 gaagcgggca agagctgcgg tgctggctcc ctgatgccag ccctggagga gccccagccg 3481 gaccaggctt tccgcatgtt tccggatatc tgtacctcac accagagacc cttttttaga 3541 gaaaatcaac agatcaccgt cTCTAGAGGG CCCTTCGAGG GCAGAGGAAG TCTTCTAACA 3601 TGCGGTGACG TGGAGGAGAA TCCCGGCCCT ATGGTGAGCA AGGGCGAGGA GCTGTTCACC 3661 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG 3721 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC 3781 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG 3841 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC 3901 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC 3961 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC 4021 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC 4081 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC 4141 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC 4201 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA 4261 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC 4321 ACTCTCGGCA TGGACGAGCT GTACAAGTAA GTTTAAACCC GCTGATCAGC CTCGACTGTG 4381 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 4441 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 4501 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 4561 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC 4621 AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 4681 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 4741 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 4801 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 4861 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 4921 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 4981 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 5041 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG 5101 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 5161 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 5221 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 5281 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 5341 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 5401 GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG 5461 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 5521 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 5581 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 5641 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 5701 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 5761 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 5821 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 5881 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 5941 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 6001 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 6061 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 6121 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG 6181 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 6241 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA 6301 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC 6361 TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC 6421 GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT 6481 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT 6541 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT 6601 AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 6661 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 6721 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 6781 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 6841 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 6901 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 6961 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 7021 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 7081 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 7141 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 7201 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 7261 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 7321 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 7381 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 7441 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 7501 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 7561 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 7621 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 7681 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 7741 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 7801 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 7861 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 7921 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 7981 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 8041 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 8101 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 8161 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 8221 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 8281 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 8341 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 8401 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 8461 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 8521 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 8581 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 8641 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 8701 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 8761 CGAAAAGTGC CACCTGACGT C

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.