Tet-pLKO-mCherry plasmid

Tet-pLKO-mCherry plasmid

PVT90288

Tet-pLKO-mCherry plasmid Information

Plasmid name: Tet-pLKO-mCherry
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,Tetracycline induction
Fragment type: shRNA
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: H1-2O2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: hPGK-R
Copy number: High
Induction:

Tet-pLKO-mCherry plasmid Description

Tet-pLKO-mCherry This is a plasmid suitable for interference silencing in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

Tet-pLKO-mCherry plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt 541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt 601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct 661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa 721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt 781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat 841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat 901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc 961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag 1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc 1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 2401 ggatccGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC 2461 ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC 2521 GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT 2581 GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC 2641 TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC 2701 AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC 2761 TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC 2821 GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC 2881 CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC 2941 CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC 3001 GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG 3061 GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG 3121 TAAgatatAT ccgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc 3181 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 3241 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 3301 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 3361 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 3421 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 3481 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 3541 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 3601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3661 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 3721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 3781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 3841 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 3901 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3961 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 4021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 4141 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4261 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4381 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 4441 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 4501 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 5041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 5221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 5401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 5461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 5521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 6421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 6481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 6541 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 6601 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 6661 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 6721 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 6781 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 6841 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 6901 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 6961 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7141 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 7201 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 7261 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 7321 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 7381 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 7441 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 7501 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 7561 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 7621 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg 7681 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat 7741 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag 7801 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg 7861 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca 7921 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg 7981 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca 8041 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa 8101 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg 8161 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa 8221 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa 8281 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg 8341 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg 8401 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga 8461 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg 8521 atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta 8581 tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt 8641 ccctatcagt gatagagaca ccggtgtgtt gtaaatgagc acacaaaata cacatgctaa 8701 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt 8761 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata 8821 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca 8881 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt 8941 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta 9001 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat 9061 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt 9121 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct 9181 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct 9241 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca 9301 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta 9361 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca 9421 tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata 9481 tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa 9541 tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt 9601 ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa 9661 attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa 9721 caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt 9781 caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg 9841 cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat 9901 aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg 9961 agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat 10021 cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat 10081 taactccata tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat 10141 atagagccgg attttttgga atgctattta agcgttttag atttgataat tttcaatcaa 10201 ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg 10261 ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa 10321 taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat 10381 ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt 10441 cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt 10501 ttaaatcttt tttagcttct tgctcttttt tgtagaattc tcgacctcga gacaaatggc 10561 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag 10621 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa 10681 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga 10741 ggggg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.