pT7-TrxA-6×His-IFT88-6×His pla

pT7-TrxA-6×His-IFT88-6×His plasmid

PVT90286

pT7-TrxA-6×His-IFT88-6×His plasmid Information

Plasmid name: pT7-TrxA-6×His-IFT88(human)-6×His
Alias: NM_001353565.2;D13S1056E; DAF19; hTg737; TG737; TTC10
Gene lenght: 2499bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7ter
Reverse primer:
Copy number: High
Induction: IPTG Induction

pT7-TrxA-6×His-IFT88-6×His plasmid Description

pT7-TrxA-6×His-IFT88-6×His is a plasmid suitable for protein expression in Escherichia coli.

pT7-TrxA-6×His-IFT88-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa 5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc 5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat 5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac 5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct 5581 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag 5641 cacatggaca gcccagatct gggtaccgac gacgacgaca aggccatggc tgatatcgga 5701 tccatgaaat tcacaaacac taaggtacaa atgatgcaaa atgtgcacct ggctccagag 5761 acagatgaag atgatcttta ttccggctat aatgactaca atccaatcta tgatatcgag 5821 gaattggaga atgatgcagc ttttcagcaa gctgtgagga ctagtcatgg cagaagacct 5881 ccaataactg ctaaaatatc aagcacggca gttactagac ctatagctac tggatatggg 5941 tccaagacat ctctggcatc atcaatagga agaccaatga caggggctat tcaggatgga 6001 gttactagac ccatgacagc agtgagagca gctggtttta ccaaagcagc tttgagaggc 6061 tctgcatttg acccccttag tcagtcaagg ggccctgctt cccctttgga agccaagaaa 6121 aaagatagcc cagaggaaaa aataaagcaa ttagagaagg aagtaaatga gttggtagaa 6181 gaaagctgta ttgccaatag ttgtggagac ttaaaattgg ccttagaaaa ggcaaaagat 6241 gcaggaagaa aagagagagt cctggtgaga cagcgagaac aagttacaac tccagaaaat 6301 atcaatttgg atttaactta ctcagttctt ttcaatttgg ccagtcagta ttcagttaat 6361 gaaatgtatg ccgaagcact taacacttat caagttatag tcaaaaataa gatgtttagc 6421 aatgcaggaa tattgaaaat gaatatggga aatatctatt taaagcaaag aaattattcc 6481 aaagccatta aattctaccg aatggcatta gaccaagttc caagtgtcaa taagcaaatg 6541 aggattaaaa taatgcagaa tattggagtt acatttattc aggctggtca gtattcagat 6601 gctattaatt catatgagca cataatgagc atggcaccaa atctgaaggc aggctacaac 6661 ctaactatct gttattttgc tattggagac cgagaaaaaa tgaagaaggc attccaaaaa 6721 ttgattactg ttccattaga aattgatgaa gataaatata tttcaccaag tgatgatcct 6781 catactaact tagtaactga agctataaaa aatgatcacc tcaggcaaat ggaacgtgaa 6841 aggaaagcca tggcagaaaa atatattatg acatctgcaa aactcattgc tcctgtaatt 6901 gaaacatctt ttgctgcagg ttatgattgg tgcgtggaag tggtgaaagc ttctcaatat 6961 gtagagctag ccaatgatct ggaaataaac aaagcagtta catacttgag acaaaaagac 7021 tataaccaag ctgtagagat cttaaaagtg ttggaaaaaa aggacagtag agtgaaaagt 7081 gcagctgcaa ccaatctctc agccctgtat tatatgggaa aggattttgc acaagccagc 7141 agctatgcag atatagctgt gaactctgat agatataatc cagcagctct tactaataaa 7201 gggaatacag tttttgcaaa tggtgattat gagaaggccg ctgaattcta taaagaggct 7261 ctaagaaatg attcttcttg tactgaagca ctttataata ttggccttac ctatgagaaa 7321 ctaaatcggc tagatgaggc tttggactgt ttcctgaaac ttcacgcaat cctacgaaac 7381 agtgccgaag ttctttacca gatagcaaat atatatgaat taatggaaaa tcccagtcaa 7441 gctattgaat ggctaatgca ggtggtcagt gttattccaa ccgatcctca agttttatct 7501 aagctaggag aattatatga tcgtgaagga gataaatctc aagcatttca atattactat 7561 gagtcatata ggtattttcc ttgtaatatt gaagtcattg agtggcttgg agcctattac 7621 attgacaccc aattttggga aaaagctatt cagtactttg aaagagcttc tcttatacag 7681 cctacacaag tgaaatggca gctgatggta gctagttgtt tcagaagaag tggtaactac 7741 caaaaagcat tagatactta caaagatact cacagaaaat ttccagaaaa tgtcgaatgt 7801 ctgcgtttct tagttcgtct ctgcacagat cttggattaa aagatgctca agaatatgcc 7861 agaaaactga agaggttgga aaaaatgaaa gaaataaggg aacagcgcat aaagtcaggc 7921 agagatggca gtgggggctc ccgtggcaaa agagaaggaa gtgctagcgg tgatagtggc 7981 cagaactata gtgccagtag taaaggtgaa cgactaagtg ccagactcag agctttacct 8041 gggacaaatg aaccttatga aagtagcagt aacaaagaaa tagatgcctc ctatgtggac 8101 ccacttggcc ctcaaataga acgaccaaaa actgcagcca agaaaaggat cgatgaggat 8161 gattttgctg atgaagaatt aggagatgat ttgcttccag aactcgagca ccaccaccac 8221 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc 8281 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg 8341 ctgaaaggag gaactatatc cggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.