pLV2-CMV-CHST3-3×FLAG-Puro pla

pLV2-CMV-CHST3-3×FLAG-Puro plasmid

PVT90152

pLV2-CMV-CHST3-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-CHST3(human)-3×FLAG-Puro
Alias: NM_004273.5;C6ST; C6ST1; HSD;
Gene lenght: 1437bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-CHST3-3×FLAG-Puro plasmid Description

pLV2-CMV-CHST3-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-CHST3-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggagaa aggactcact ttgccccagg actgccggga 2881 ctttgtgcac agcctgaaga tgagaagcaa atacgccctt ttcttggttt ttgtggtgat 2941 agtttttgtc ttcatcgaaa aggaaaataa aatcatatca agggtctcag acaagctgaa 3001 gcagattccc caagctctag cagatgccaa cagcaccgac ccagccctga tcttagctga 3061 gaacgcatct ctcttgtccc tgagcgagct cgattcagcc ttctcccagc ttcagagccg 3121 tctccgcaac ctcagcttgc agctgggcgt ggagccagcc atggaggccg caggggagga 3181 agaggaagag cagagaaagg aggaggagcc gcccagaccg gccgtggcgg ggccccggcg 3241 ccacgtgctg ctcatggcca ccacgcgcac cggctcctcg ttcgtgggcg agttcttcaa 3301 ccagcagggc aacatcttct acctcttcga gccgctgtgg cacatcgagc gcacagtgtc 3361 cttcgagccg gggggcgcca acgccgcggg ctcggccctg gtgtaccgcg acgtgctcaa 3421 gcagctcttc ctgtgcgacc tgtacgtgct ggagcacttc atcacgccgc tgcccgagga 3481 ccacctgact cagttcatgt tccgccgggg ctccagccgc tccctgtgcg aggaccccgt 3541 ctgtacgccc ttcgtcaaga aggtcttcga gaagtaccac tgcaagaacc gccgctgcgg 3601 ccccctcaac gtgacgctgg ccgcagaggc ctgccgccgc aaggagcaca tggccctcaa 3661 ggcggtgcgc atccggcagc tggagttcct gcagccgctg gccgaggacc cccgcctgga 3721 cctgcgcgtc atccagctgg tgcgcgaccc ccgggccgtg ctggcctcgc gcatggtggc 3781 cttcgccggc aagtataaga cctggaagaa gtggctggac gacgagggcc aggacggcct 3841 gagggaagag gaggtgcagc ggctgcgggg caactgcgag agcatccgcc tgtccgcgga 3901 gctggggctg cggcagcccg cctggctgcg gggccgctac atgctggtgc gctacgagga 3961 cgtggcacgc gggccgctgc agaaggcccg cgagatgtac cgcttcgccg gcatccccct 4021 gaccccgcag gtggaagact ggatccaaaa gaacacgcag gcggcccacg acggcagcgg 4081 catctactcc acgcagaaga actcctcgga gcagttcgag aagtggcgct tcagcatgcc 4141 cttcaagctg gcccaggtgg tgcaggccgc ctgcggccct gccatgcgcc tcttcggcta 4201 caaactggcg cgggacgccg ccgccctcac caaccgctca gtcagcctgc tggaggagag 4261 gggcaccttc tgggtcacgG GTGGAGGCgg atccGACTAC AAAGACCATG ACGGTGATTA 4321 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG tagtctagat aattctaccg 4381 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 4441 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 4501 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 4561 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 4621 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 4681 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 4741 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 4801 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 4861 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 4921 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4981 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 5041 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5101 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5161 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5221 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5281 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5341 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5401 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5461 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 5521 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5581 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5641 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5701 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5761 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5821 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5881 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5941 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6001 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6061 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 6121 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 6181 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 6241 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6301 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 6361 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 6421 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 6481 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 6541 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 6601 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 6661 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 6721 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 6781 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6841 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6901 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6961 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 7021 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 7081 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 7141 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 7201 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 7261 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 7321 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 7381 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 7441 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 7501 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 7561 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 7621 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 7681 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 7741 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 7801 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 7861 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 7921 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 7981 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 8041 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 8101 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 8161 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 8221 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 8281 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 8341 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 8401 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 8461 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 8521 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 8581 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 8641 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 8701 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 8761 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 8821 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 8881 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 8941 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 9001 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 9061 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 9121 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 9181 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 9241 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9301 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9361 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 9421 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 9481 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 9541 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.