pLV3-CMV-NAT10-Myc-Neo plasmid

pLV3-CMV-NAT10-Myc-Neo plasmid

PVT89997

pLV3-CMV-NAT10-Myc-Neo plasmid Information

Plasmid name: pLV3-CMV-NAT10(human)-Myc-Neo
Alias: NM_024662.3;ALP; Kre33; NET43
Gene lenght: 3075bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NAT10-Myc-Neo plasmid Description

pLV3-CMV-NAT10-Myc-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NAT10-Myc-Neo plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgcatcgg aaaaaggtgg ataaccgaat ccggattctc 2341 attgagaatg gagtagctga gcggcaaaga tctctctttg ttgtagttgg ggatcgagga 2401 aaagatcagg tggtaatact tcatcacatg ttatccaaag caactgtgaa ggctcggcct 2461 tcagtgctgt ggtgttataa gaaagagctg gggtttagca gtcaccggaa gaaaagaatg 2521 cgacagctgc agaagaaaat aaagaatgga acactgaaca taaagcagga cgaccccttt 2581 gaactcttca tagcagccac aaacattcgc tactgctact acaacgagac ccacaagatc 2641 ctgggcaata ccttcggcat gtgtgtgctg caggattttg aagccttaac tccaaacttg 2701 ctggccagga ctgtagaaac agtggaaggt ggtgggctag tggtcatcct cctacggacc 2761 atgaactcac tcaagcaatt gtacacagtg actatggatg tgcattccag gtacagaact 2821 gaggcccatc aggatgtggt gggaagattt aatgaaaggt ttattctgtc tctggcctct 2881 tgtaagaagt gtctcgtcat tgatgaccag ctcaacatcc tgcccatctc ctcccacgtt 2941 gccaccatgg aggccctgcc tccccagact ccggatgaga gtcttggtcc ttctgatctg 3001 gagctgaggg agttgaagga gagcttgcag gacacccagc ctgtgggtgt gttggtggac 3061 tgctgtaaga ctctagacca ggccaaagct gtcttgaaat ttatcgaggg catctctgaa 3121 aagaccctga ggagtactgt tgcactcaca gctgctcgag gacggggaaa atctgcagcc 3181 ctgggattgg cgattgctgg ggcggtggca tttgggtact ccaatatctt tgttacctcc 3241 ccaagccctg ataacctcca tactctgttt gaatttgtat ttaaaggatt tgatgctctg 3301 caatatcagg aacatctgga ttatgagatt atccagtctc taaatcctga atttaacaaa 3361 gcagtgatca gagtgaatgt atttcgagaa cacaggcaga ctattcagta tatacatcct 3421 gcagatgctg tgaagctggg ccaggctgaa ctagttgtga ttgatgaagc tgccgccatc 3481 cccctcccct tggtgaagag cctacttggc ccctaccttg ttttcatggc atccaccatc 3541 aatggctatg agggcactgg ccggtcactg tccctcaagc taattcagca gctccgtcaa 3601 cagagcgccc agagccaggt cagcaccact gctgagaata agaccacgac gacagccaga 3661 ttggcatcag cgcggacact gtatgaggtt tccctccagg agtcaatccg atacgcccct 3721 ggggatgcag tggagaagtg gctgaatgac ttgctgtgcc tggattgcct caacatcact 3781 cggatagtct caggctgccc cttgcctgaa gcttgtgaac tgtactatgt taatagagat 3841 accctctttt gctaccacaa ggcctctgaa gttttcctcc aacggcttat ggccctctac 3901 gtggcttctc actacaagaa ctctcccaat gatctccaga tgctctccga tgcacctgct 3961 caccatctct tctgccttct gcctcccgtg ccccccaccc agaatgccct tccagaagtg 4021 cttgctgtta tccaggtgtg ccttgaaggg gagatttctc gccagtccat cttgaacagt 4081 ctgtctcgag gcaagaaggc ttcaggggac ctgattccat ggacagtgtc agaacagttc 4141 caagatccag actttggtgg tctgtctggt ggaagggtcg ttcgcattgc tgttcaccca 4201 gattatcaag ggatgggcta tggcagccgt gctctgcagc tgctgcagat gtactatgaa 4261 ggcaggtttc cttgtctgga ggaaaaggtc cttgagacac cacaggaaat tcacaccgta 4321 agcagcgagg ctgtcagctt gttggaagag gtcatcactc cccggaagga cctgcctcct 4381 ttactcctca aattgaatga gaggcctgcc gaacgcctgg attacctggg tgtttcctat 4441 ggcttgaccc ccaggctcct caagttctgg aaacgagctg gatttgttcc tgtttatctg 4501 agacagaccc cgaatgacct gaccggagag cactcgtgca tcatgctgaa gacgctcact 4561 gatgaggatg aggctgacca gggaggctgg cttgcagcct tctggaaaga tttccgacgg 4621 cggttcctag ccttgctctc ctaccagttc agtaccttct ctccttccct ggctctgaac 4681 atcattcaga acaggaacat ggggaagcca gcccagcctg ccctgagccg ggaggagctg 4741 gaagcactct tcctccccta tgacctgaag cggctggaga tgtattcacg gaatatggtg 4801 gactatcacc tcatcatgga catgatcccg gccatctctc gcatctattt cctgaaccag 4861 ctgggggacc tggccctgtc tgcggctcag tcggctcttc tcttggggat tggcctgcag 4921 cataagtctg tggaccagct ggaaaaggag attgagctgc cctcgggcca gttgatggga 4981 cttttcaacc ggatcatccg caaagttgtg aagctattta atgaagttca ggaaaaggcc 5041 attgaggagc agatggtggc agcgaaggat gtggtcatgg agcccacgat gaagaccctc 5101 agtgacgacc tagatgaagc agcaaaggaa tttcaggaga aacacaagaa ggaagtaggg 5161 aagctgaaga gcatggacct ctctgaatac ataatccgtg gggacgatga agagtggaat 5221 gaagttttga acaaagctgg gccgaacgcc tcgatcatca gcctgaaaag tgacaagaaa 5281 aggaagttag aggccaaaca agaacccaaa cagagcaaga agttgaagaa cagagagaca 5341 aagaacaaaa aagatatgaa actgaagcgg aagaaaGGAT CCGAGCAGAA GCTGATCTCA 5401 GAGGAGGACC TGTAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA 5461 GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT 5521 GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT 5581 TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT 5641 CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA 5701 CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT 5761 CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG 5821 AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG 5881 CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT 5941 TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGGCCACC atggctgaac aagatggatt 6001 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 6061 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 6121 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 6181 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 6241 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 6301 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 6361 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 6421 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 6481 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 6541 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 6601 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 6661 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 6721 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaGTCGA 6781 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 6841 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 6901 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 6961 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 7021 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 7081 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 7141 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT 7201 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 7261 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 7321 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGTACCTT 7381 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG 7441 GACTGGAAGG GCTAATTCAC TCCCAACGAA AATAAGATCT GCTTTTTGCT TGTACTGGGT 7501 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC 7561 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG 7621 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA 7681 GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA 7741 GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 7801 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 7861 ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 7921 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 7981 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGACT TTTGCAGAGA 8041 CGGCCCAAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 8101 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 8161 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 8221 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 8281 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 8341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 8401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 8461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 8521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 8581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 8641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 8701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 8761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 8821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 8881 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 8941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 9001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 9061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 9121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 9181 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 9241 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 9301 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 9361 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 9421 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 9481 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 9541 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 9601 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 9661 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 9721 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 9781 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 9841 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 9901 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 9961 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 10021 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 10081 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 10141 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 10201 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 10261 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 10321 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 10381 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 10441 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 10501 GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 10561 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG 10621 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC 10681 CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.