pLV3-CMV-Cgref1-3×FLAG-ZsGreen

pLV3-CMV-Cgref1-3×FLAG-ZsGreen1-Puro plasmid

PVT89859

pLV3-CMV-Cgref1-3×FLAG-ZsGreen1-Puro plasmid Information

Plasmid name: pLV3-CMV-Cgref1(mouse)-3×FLAG-ZsGreen1-Puro
Alias: NM_001160149.1;1110004G24Rik; Cgr11
Gene lenght: 840bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cgref1-3×FLAG-ZsGreen1-Puro plasmid Description

pLV3-CMV-Cgref1-3×FLAG-ZsGreen1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Cgref1-3×FLAG-ZsGreen1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc 1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aAATTTTCGG 1921 GTTTATTACA GGGACAGCAG AGATCCAGTT TATCGATAAG CTTGGGAGTT CCGCGTTACA 1981 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 2041 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 2101 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG 2161 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 2221 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 2281 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 2341 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 2401 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 2461 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA 2521 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTACTAGA GGATCTATTT CCGGTGAATT 2581 CGCCACCatg ttccagtggc tgatgcaagc gttgatgctg ccactattgc tgctcccttt 2641 aggtcgagct gctcccaagg atggagttgc aaggttggac cctgaggtac aacagcagct 2701 cacacccaac cctttccagc caggccctga gcagctccga catctgcaga attatctcaa 2761 gggactagag aagatggaag aggatcctga gcacatggac cgggagcaag tcctgctttc 2821 gctctttgct cttcatgact atgaccagaa tggacagctg gacggcctgg agctactgtc 2881 catgctgaca gcagctctgg cccctggagc tgcacacttt cccatcaacc cggtgatcct 2941 agtagtagac tcggtgctcg agactcagga cctggatgga gacgggctca tgactcctgc 3001 agagctcatc aacttcccag aagtccccaa acacacagag tcccttcccc cagctctcca 3061 ggagccacaa cctgctggaa gtcagccact tttagccaac agtccactgc agtcagaaac 3121 ccagcagtcc ctggggacta aagaaattag gagccaggta gaggccaaga gggcgtcctt 3181 ggagcctgaa caggaggctg gacatcagac agagggaaaa gtagataccc taagccctga 3241 agaggaggct aggggacagg cagagtctga aggagatgtt ccaggtccca gagaaggtgc 3301 tgaggaacag gtggagatca aggacaatga aggagaagcc aaagaactgc tggtggaaac 3361 actggagagc ctaaacactc caaatgaggc tgaggctcat agcatccaat tggagaacga 3421 tgagataGGA TCCTCGGTAC CAAGCTTAAG TGACTACAAG GATGACGATG ACAAGGATTA 3481 CAAAGACGAC GATGATAAGG ACTATAAGGA TGATGACGAC AAATAAAGAT CCATCGATAC 3541 TAGTAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG 3601 TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG 3661 GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG 3721 AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA 3781 GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT 3841 GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT 3901 GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG 3961 CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG 4021 CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC 4081 CGGCGCCTAC TCTAGCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 4141 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 4201 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 4261 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 4321 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 4381 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 4441 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 4501 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 4561 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 4621 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 4681 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 4741 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCg cggccgctga 4801 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga 4861 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 4921 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 4981 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5041 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5101 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5161 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5221 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5281 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5341 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5401 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc 5461 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5521 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5581 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5641 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5701 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5761 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5821 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 5881 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5941 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg 6001 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat 6061 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6121 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt 6181 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6241 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6301 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6361 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6541 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc 6601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6661 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat 6721 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6781 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 8941 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 9001 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 9061 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 9121 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 9181 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 9241 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 9301 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.