pLV3-CMV-SPP1-3×FLAG-CopGFP-Pu

pLV3-CMV-SPP1-3×FLAG-CopGFP-Puro plasmid

PVT89598

pLV3-CMV-SPP1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SPP1(human)-3×FLAG-CopGFP-Puro
Alias:
Gene lenght: 942bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SPP1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SPP1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SPP1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gaattgcagt gatttgcttt 2341 tgcctcctag gcatcacctg tgccatacca gttaaacagg ctgattctgg aagttctgag 2401 gaaaagcagc tttacaacaa atacccagat gctgtggcca catggctaaa ccctgaccca 2461 tctcagaagc agaatctcct agccccacag aatgctgtgt cctctgaaga aaccaatgac 2521 tttaaacaag agacccttcc aagtaagtcc aacgaaagcc atgaccacat ggatgatatg 2581 gatgatgaag atgatgatga ccatgtggac agccaggact ccattgactc gaacgactct 2641 gatgatgtag atgacactga tgattctcac cagtctgatg agtctcacca ttctgatgaa 2701 tctgatgaac tggtcactga ttttcccacg gacctgccag caaccgaagt tttcactcca 2761 gttgtcccca cagtagacac atatgatggc cgaggtgata gtgtggttta tggactgagg 2821 tcaaaatcta agaagtttcg cagacctgac atccagtacc ctgatgctac agacgaggac 2881 atcacctcac acatggaaag cgaggagttg aatggtgcat acaaggccat ccccgttgcc 2941 caggacctga acgcgccttc tgattgggac agccgtggga aggacagtta tgaaacgagt 3001 cagctggatg accagagtgc tgaaacccac agccacaagc agtccagatt atataagcgg 3061 aaagccaatg atgagagcaa tgagcattcc gatgtgattg atagtcagga actttccaaa 3121 gtcagccgtg aattccacag ccatgaattt cacagccatg aagatatgct ggttgtagac 3181 cccaaaagta aggaagaaga taaacacctg aaatttcgta tttctcatga attagatagt 3241 gcatcttctg aggtcaatgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3301 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3361 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3421 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3481 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3541 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3601 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3661 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3721 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3781 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3841 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3901 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3961 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4021 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4081 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4141 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4201 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4261 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4321 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4381 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4441 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4501 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4561 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4621 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4681 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4741 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4801 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4861 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4921 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4981 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5041 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5101 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5161 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5221 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5281 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5341 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5401 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5461 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5521 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5581 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5641 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5701 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5761 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5821 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5881 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5941 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6001 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6061 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6121 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6181 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6241 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6421 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6481 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6541 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6601 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7441 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8761 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8821 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8881 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8941 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9001 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9061 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9121 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9181 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.