pLV3-EF1a-Areg-3×FLAG-T2A-CopG

pLV3-EF1a-Areg-3×FLAG-T2A-CopGFP plasmid

PVT89486

pLV3-EF1a-Areg-3×FLAG-T2A-CopGFP plasmid Information

Plasmid name: pLV3-EF1a-Areg(mouse)-3×FLAG-T2A-CopGFP
Alias: NM_009704.4;AR; Mcub; Sdgf;
Gene lenght: 744bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-Areg-3×FLAG-T2A-CopGFP plasmid Description

pLV3-EF1a-Areg-3×FLAG-T2A-CopGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-Areg-3×FLAG-T2A-CopGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 2461 agctgtgacc ggcgcctact ctagagctag cgaattcgcc accatgagaa ctccgctgct 2521 accgctggcg cgctcagtgc tgttgctgct ggtcttaggc tcaggccatt atgcagctgc 2581 tttggagctc aatgacccca gctcagggaa aggcgaatcg ctttctgggg accacagtgc 2641 cggtggactt gagctttctg tgggaagaga ggtttccacc ataagcgaaa tgccttctgg 2701 cagtgaactc tccacagggg actacgacta ctcagaggag tatgataatg aaccacaaat 2761 atccggctat attatagatg attcagtcag agttgaacag gtgattaagc ccaagaaaaa 2821 caagacagaa ggagaaaagt ctacagaaaa acccaaaagg aagaaaaagg gaggcaaaaa 2881 tggaaaaggc agaaggaata agaagaaaaa gaatccatgc actgccaagt ttcagaactt 2941 ttgcattcat ggcgaatgca gatacatcga gaacctggag gtggtgacat gcaattgtca 3001 tcaagattac tttggtgaac ggtgtggaga aaaatccatg aagactcaca gcgaggatga 3061 caaggaccta tccaagattg cagtagtagc tgtcactatc tttgtctctg ccatcatcct 3121 cgcagctatt ggcatcggca tcgttatcac agtgcacctt tggaaacgat acttcaggga 3181 atatgaagga gaaacagaag aaagaaggag gcttcgacaa gaaaacggga ctgtgcatgc 3241 cattgccggt ggtggtggtt ccgattacaa ggaccacgac ggggattaca aggaccacga 3301 cattgattac aaggatgatg atgacaaggg atccgcggcc gctgagggca gaggaagtct 3361 tctaacatgc ggtgacgtgg aggagaatcc cggcccttcc ggaatggaga gcgacgagag 3421 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt 3481 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa 3541 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg 3601 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa 3661 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt 3721 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac 3781 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt 3841 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag 3901 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc 3961 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga 4021 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat 4081 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc 4141 cggctccacc ggatctcgct agagctgaat ctaagtcgac aatcaacctc tggattacaa 4201 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 4261 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 4321 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 4381 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 4441 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 4501 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 4561 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 4621 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 4681 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 4741 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 4801 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 4861 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 4921 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 4981 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 5041 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 5101 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 5161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 5281 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 5341 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 5401 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 5461 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 5521 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 5581 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 5641 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5701 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 5761 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5821 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6301 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6421 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6481 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6541 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6601 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6661 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6721 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 6781 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6841 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6901 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 6961 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7021 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7081 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7141 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7201 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7261 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7321 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7381 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7441 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7501 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7561 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7621 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 7681 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 7741 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 7801 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 7861 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 7921 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 7981 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 8041 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.