pGreenIIM-DR5-GUS-35S-EGFP-DHF

pGreenIIM-DR5-GUS-35S-EGFP-DHFR plasmid

PVT89294

pGreenIIM-DR5-GUS-35S-EGFP-DHFR plasmid Information

Plasmid name: pGreenIIM-DR5-GUS-35S-EGFP-DHFR
Alias: -
Gene lenght: 3870bp
Host: Plant
Use(s): Protein Expression
Fragment type: ORF
Species: synthesis
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: 35S
Replicon: pSa
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: EGFP-F3
Reverse primer:
Copy number: High
Induction:

pGreenIIM-DR5-GUS-35S-EGFP-DHFR plasmid Description

pGreenIIM-DR5-GUS-35S-EGFP-DHFR This is a plasmid suitable for protein expression in plants.

pGreenIIM-DR5-GUS-35S-EGFP-DHFR plasmid Sequence

1 CCGACAAAAG GCCGACAAAA GGCCGACAAA AGGCCGACAA AAGGCCGACA AAAGGCCGAC 61 AAAAGGCCGA CAAAAGGCCG ACAAAAGGCC GACAAAAGGC CGACAAAAGG GGGCAGGCCT 121 CGATAAGCTT GATATCGAAT TAATTCCTGC AGCCCCGCAA GACCCTTCCT CTATATAAGG 181 AAGTTCATTT CATTTGGAGA GGTATTTTTA CAACAATTAC CAACAACAAC AAACAACAAA 241 CAACATTACA ATTACTATTT ACAATTACAA TTACAGGGGA TCCGAAATCC AACTCCATAC 301 TGAAGCTTAT GGCTCCAAAG AAGAAGAGAA AGGTCGAGAC AAAAGGGAGA CAAAAGGGAG 361 ACAAAAGGGA GACAAAAGGG AGACAAAAGG GAGACAAAAG GGAGACAAAA GGGAGACAAA 421 AGGGAGACAA AAGGGAGACA ATGTTACGTC CTGTAGAAAC CCCAACCCGT GAAATCAAAA 481 AACTCGACGG CCTGTGGGCA TTCAGTCTGG ATCGCGAAAA CTGTGGAATT GATCAGCGTT 541 GGTGGGAAAG CGCGTTACAA GAAAGCCGGG CAATTGCTGT GCCAGGCAGT TTTAACGATC 601 AGTTCGCCGA TGCAGATATT CGTAATTATG CGGGCAACGT CTGGTATCAG CGCGAAGTCT 661 TTATACCGAA AGGTTGGGCA GGCCAGCGTA TCGTGCTGCG TTTCGATGCG GTCACTCATT 721 ACGGCAAAGT GTGGGTCAAT AATCAGGAAG TGATGGAGCA TCAGGGCGGC TATACGCCAT 781 TTGAAGCCGA TGTCACGCCG TATGTTATTG CCGGGAAAAG TGTACGTATC ACCGTTTGTG 841 TGAACAACGA ACTGAACTGG CAGACTATCC CGCCGGGAAT GGTGATTACC GACGAAAACG 901 GCAAGAAAAA GCAGTCTTAC TTCCATGATT TCTTTAACTA TGCCGGAATC CATCGCAGCG 961 TAATGCTCTA CACCACGCCG AACACCTGGG TGGACGATAT CACCGTGGTG ACGCATGTCG 1021 CGCAAGACTG TAACCACGCG TCTGTTGACT GGCAGGTGGT GGCCAATGGT GATGTCAGCG 1081 TTGAACTGCG TGATGCGGAT CAACAGGTGG TTGCAACTGG ACAAGGCACT AGCGGGACTT 1141 TGCAAGTGGT GAATCCGCAC CTCTGGCAAC CGGGTGAAGG TTATCTCTAT GAACTGTGCG 1201 TCACAGCCAA AAGCCAGACA GAGTGTGATA TCTACCCGCT TCGCGTCGGC ATCCGGTCAG 1261 TGGCAGTGAA GGGCGAACAG TTCCTGATTA ACCACAAACC GTTCTACTTT ACTGGCTTTG 1321 GTCGTCATGA AGATGCGGAC TTGCGTGGCA AAGGATTCGA TAACGTGCTG ATGGTGCACG 1381 ACCACGCATT AATGGACTGG ATTGGGGCCA ACTCCTACCG TACCTCGCAT TACCCTTACG 1441 CTGAAGAGAT GCTCGACTGG GCAGATGAAC ATGGCATCGT GGTGATTGAT GAAACTGCTG 1501 CTGTCGGCTT TAACCTCTCT TTAGGCATTG GTTTCGAAGC GGGCAACAAG CCGAAAGAAC 1561 TGTACAGCGA AGAGGCAGTC AACGGGGAAA CTCAGCAAGC GCACTTACAG GCGATTAAAG 1621 AGCTGATAGC GCGTGACAAA AACCACCCAA GCGTGGTGAT GTGGAGTATT GCCAACGAAC 1681 CGGATACCCG TCCGCAAGGT GCACGGGAAT ATTTCGCGCC ACTGGCGGAA GCAACGCGTA 1741 AACTCGACCC GACGCGTCCG ATCACCTGCG TCAATGTAAT GTTCTGCGAC GCTCACACCG 1801 ATACCATCAG CGATCTCTTT GATGTGCTGT GCCTGAACCG TTATTACGGA TGGTATGTCC 1861 AAAGCGGCGA TTTGGAAACG GCAGAGAAGG TACTGGAAAA AGAACTTCTG GCCTGGCAGG 1921 AGAAACTGCA TCAGCCGATT ATCATCACCG AATACGGCGT GGATACGTTA GCCGGGCTGC 1981 ACTCAATGTA CACCGACATG TGGAGTGAAG AGTATCAGTG TGCATGGCTG GATATGTATC 2041 ACCGCGTCTT TGATCGCGTC AGCGCCGTCG TCGGTGAACA GGTATGGAAT TTCGCCGATT 2101 TTGCGACCTC GCAAGGCATA TTGCGCGTTG GCGGTAACAA GAAAGGGATC TTCACTCGCG 2161 ACCGCAAACC GAAGTCGGCG GCTTTTCTGC TGCAAAAACG CTGGACTGGC ATGAACTTCG 2221 GTGAAAAACC GCAGCAGGGA GGCAAACAAT GACTCGAGAG ATCCGATCCC CGATCGTTCA 2281 AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC 2341 ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA 2401 TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA 2461 AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTGAtga 2521 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg 2581 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga 2641 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc 2701 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga 2761 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga 2821 cccttcctct atataaggaa gttcatttca tttggagagg acctcgacct caacacaaca 2881 tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa 2941 tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatactc 3001 gagggggatc cccaatactt gtatggccgc ggccgcatgg tgagcaaggg cgaggagctg 3061 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 3121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 3181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 3241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 3301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 3361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 3421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 3481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 3541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 3601 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 3661 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 3721 gggatcactc tcggcatgga cgagctgtac aagtagTCTA GATCCGATCC CCGATCGTTC 3781 AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT 3841 CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT 3901 ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA 3961 AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTCTAG 4021 AGCGGCCGCC ACCGCGGTGG AGCTCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTGCGC 4081 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 4141 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 4201 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 4261 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 4321 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 4381 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 4441 TGAAGGCCTT GACAGGATAT ATTGGCGGGT AAACTAAGTC GCTGTATGTG TTTGTTTGAG 4501 ATCTCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 4561 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 4621 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 4681 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4741 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 4801 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4861 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 4921 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 4981 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 5041 TTACCTTCGG AAGAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 5101 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 5161 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 5221 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 5281 TTAAATCAAT CTAAAGTATA TATGTGTAAC ATTGGTCTAG TGATTAGAAA AACTCATCGA 5341 GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA 5401 GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT 5461 GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT 5521 CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC GGTGAGAATG 5581 GCAAAAGTTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT 5641 CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA 5701 ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA 5761 ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA 5821 ATGCTGTTTT CCCTGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA GTACGGATAA 5881 AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT 5941 CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG 6001 GCTTCCCATA CAATCGGTAG ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT 6061 TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTTGAG CAAGACGTTT 6121 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA 6181 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA 6241 CGTGGCTTTG TTGAATAAAT CGAACTTTTG CTGAGTTGAA GGATCAGATC ACGCATCTTC 6301 CCGACAACGC AGACCGTTCC GTGGCAAAGC AAAAGTTCAA AATCACCAAC TGGTCCACCT 6361 ACAACAAAGC TCTCATCAAC CGTGGCTCCC TCACTTTCTG GCTGGATGAT GGGGCGATTC 6421 AGGCGATCCC CATCCAACAG CCCGCCGTCG AGCGGGCTTT TTTATCCCCG GAAGCCTGTG 6481 GATAGAGGGT AGTTATCCAC GTGAAACCGC TAATGCCCCG CAAAGCCTTG ATTCACGGGG 6541 CTTTCCGGCC CGCTCCAAAA ACTATCCACG TGAAATCGCT AATCAGGGTA CGTGAAATCG 6601 CTAATCGGAG TACGTGAAAT CGCTAATAAG GTCACGTGAA ATCGCTAATC AAAAAGGCAC 6661 GTGAGAACGC TAATAGCCCT TTCAGATCAA CAGCTTGCAA ACACCCCTCG CTCCGGCAAG 6721 TAGTTACAGC AAGTAGTATG TTCAATTAGC TTTTCAATTA TGAATATATA TATCAATTAT 6781 TGGTCGCCCT TGGCTTGTGG ACAATGCGCT ACGCGCACCG GCTCCGCCCG TGGACAACCG 6841 CAAGCGGTTG CCCACCGTCG AGCGCCTTTG CCCACAACCC GGCGGCCGGC CGCAACAGAT 6901 CGTTTTATAA ATTTTTTTTT TTGAAAAAGA AAAAGCCCGA AAGGCGGCAA CCTCTCGGGC 6961 TTCTGGATTT CCGATCCCCG GAATTAGATC TTGGCAGGAT ATATTGTGGT GTAACGTTAT 7021 CGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT 7081 ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC 7141 CTATAAGAAC CCTAATTTCC CTTATCGGGA AACTACTCAC ACATTATTTA TGGAGAAAAT 7201 AGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG CGTACCGAAT TAATTCGAGC 7261 TCGGTACCCC TGGATTTTGG TTTTAGGAAT TAGAAATTTT ATTGATAGAA GTATTTTACA 7321 AATACAAATA CATACTAAGG GTTTCTTATA TGCTCAACAC ATGAGCGAAA CCCTATAAGA 7381 ACCCTAATTC CCTTATCTGG GAACTACTCA CACATTATTA TAGAGAGAGA TAGATTTGTA 7441 GAGAGAGACT GGTGATTTCA GCGGGGGATT CAGCGCCGAC GGGACGTAAA CAAAGGACGT 7501 CCCGCGAAGG ATCTAAAGCC AGCAAAAGTC CCATGGTCTT ATAAAAATGC ATAGCTTTAG 7561 GAGGGGAGCA GAGAACTTGA AAGCATCTTC CTGTTAGTCT TTCTTCTCGT AGACTTCAAA 7621 CTTATACTTG ATGCCTTTTT CCTCCTGGAC CTCAGAGAGG ACGCCTGGGT ATTCTGGGAG 7681 AAGTTTATAT TTCCCCAAAT CAATTTCTGG GAAAAACGTG TCACTTTCAA ATTCCTGCAT 7741 GATCCTTGTC ACAAAGAGTC TGAGGTGGCC TGGTTGATTC ATGGCTTCCT GGTAAACAGA 7801 ACTGCCTCCG ACTATCCAAA CCATGTCTAC TTTACTTGCC AATTCCGGTT GTTCAATAAG 7861 TCTTAAGGCA TCATCCAAAC TTTTGGCAAG AAAATGAGCT CCTCGTGGTG GTTCTTTGAG 7921 TTCTCTACTG AGAACTATAT TAATTCTGTC CTTTAAAGGT CGATTCTTCT CAGGAATGGA 7981 GAACCAGGTT TTCCTACCCA TAATCACCAG ATTCTGTTTA CCTTCCACTG AAGAGGTTGT 8041 GGTCATTCTT TGGAAGTACT TGAACTCGTT CCTGAGCGGA GGCCAGGGTC GGTCTCCGTT 8101 CTTGCCAATC CCCATATTTT GGGACACGGC GACGATGCAG TTCAATGGTC GAACCATGAT 8161 GGCAAATTCT AGGGGTACCC AGCTTGGGCT GTCCTCTCCA AATGAAATGA ACTTCCTTAT 8221 ATAGAGGAAG GGTCTTGCGA AGGATAGTGG GATTGTGCGT CATCCCTTAC GTCAGTGGAG 8281 ATGTCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG 8341 CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAATGATA 8401 GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGAGCCAC CTTCCTTTTC TACTGTCCTT 8461 TCGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA AATTACCCTT 8521 TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAC ATTTTTGGAG 8581 TAGGGGTACG ATAACATTAA CGCTTACAAT TTCCATTCGC CATTCAGGCT GCGCAACTGT 8641 TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT 8701 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 8761 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGGTAC CGGGCCCGTA 8821 TGGTTAATTA AGACACAGGC GCGCCTAGTT GGAATAGGAT TTCGAATTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.