pCMV-Dtnb-circRNA-EGFP-Neo pla

pCMV-Dtnb-circRNA-EGFP-Neo plasmid

PVT89290

pCMV-Dtnb-circRNA-EGFP-Neo plasmid Information

Plasmid name: pCMV-Dtnb(mouse)-circRNA-EGFP-Neo
Alias: mmu_circ_0000349
Gene lenght: 514bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: circRNA
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pCMV-Dtnb-circRNA-EGFP-Neo plasmid Description

pCMV-Dtnb-circRNA-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Dtnb-circRNA-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcTAATAC TTTCAGTGAG GGCCGAGGCA 661 AGTTGACCGT GTTTTCAGTT AAAGCTATGT TAGCAACCAT GTGTGGTGGA AAAATGCTGG 721 ACAAATTGAG ATACATTTTC TCCCAGATGT CAGATTCCAA TGGCTTAATG ATGTTTGGAA 781 AGCTTGACCA GTTCCTGAAG GAAGCCCTGA AGCTCCCAAC AGCTGTCTTT GAAGGGCCAT 841 CCTTTGGTTA CACAGAGCAT GCAGTCCGTA CCTGTTTTCC GCAGCAGAAG AAGATAATGC 901 TGAATATGTT TTTAGACACC ATGATGGCTG ATCCTCCTCC CCAGTGCCTT GTCTGGCTAC 961 CTCTCATGCA CAGGCTTGCC CATGTTGAGA ATGTCTTCCA TCCTGTGGAA TGCTCCTACT 1021 GTCACTGCGA GAGCATGATG GGCTTCCGAT ACCGATGCCA GCAGTGCCAC AACTACCAGC 1081 TCTGCCAGAA CTGCTTTTGG CGTGGCCATG CCAGCGGCGC TCACAGCAAC CAGCACCAGA 1141 TGAAGGAGCA TTCCTCTTGG GTAAGAACAA CTggatccac cggtcgccac catggtgagc 1201 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 1261 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 1321 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 1381 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 1441 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 1501 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 1561 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1621 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 1681 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 1741 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 1801 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 1861 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc 1921 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 1981 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 2041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 2161 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 2221 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2281 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 2341 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 2401 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2461 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2521 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2581 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2701 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 2761 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 2821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 2881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 2941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3121 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3301 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 3361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3421 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3661 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3841 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3901 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 3961 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4021 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4081 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4141 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4201 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4261 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4321 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 4381 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4441 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4501 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4561 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4621 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4681 caaatactgt Tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4741 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4801 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4861 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4921 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4981 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5041 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5101 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5161 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5221 tggataaccg tattaccgcc atgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.