pLV3-CMV-KIF5B-3×HA-EF1a-Blast

pLV3-CMV-KIF5B-3×HA-EF1a-Blast plasmid

PVT88656

pLV3-CMV-KIF5B-3×HA-EF1a-Blast plasmid Information

Plasmid name: pLV3-CMV-KIF5B(human)-3×HA-EF1a-Blast
Alias: NM_004521.3;HEL-S-61; KINH; KNS; KNS1; UKHC
Gene lenght: 2889bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KIF5B-3×HA-EF1a-Blast plasmid Description

pLV3-CMV-KIF5B-3×HA-EF1a-Blast It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KIF5B-3×HA-EF1a-Blast plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggcggac ctggccgagt gcaacatcaa agtgatgtgt 2341 cgcttcagac ctctcaacga gtctgaagtg aaccgcggcg acaagtacat cgccaagttt 2401 cagggagaag acacggtcgt gatcgcgtcc aagccttatg catttgatcg ggtgttccag 2461 tcaagcacat ctcaagagca agtgtataat gactgtgcaa agaagattgt taaagatgta 2521 cttgaaggat ataatggaac aatatttgca tatggacaaa catcctctgg gaagacacac 2581 acaatggagg gtaaacttca tgatccagaa ggcatgggaa ttattccaag aatagtgcaa 2641 gatattttta attatattta ctccatggat gaaaatttgg aatttcatat taaggtttca 2701 tattttgaaa tatatttgga taagataagg gacctgttag atgtttcaaa gaccaacctt 2761 tcagttcatg aagacaaaaa ccgagttccc tatgtaaagg ggtgcacaga gcgttttgta 2821 tgtagtccag atgaagttat ggataccata gatgaaggaa aatccaacag acatgtagca 2881 gttacaaata tgaatgaaca tagctctagg agtcacagta tatttcttat taatgtcaaa 2941 caagagaaca cacaaacgga acaaaagctg agtggaaaac tttatctggt tgatttagct 3001 ggtagtgaaa aggttagtaa aactggagct gaaggtgctg tgctggatga agctaaaaac 3061 atcaacaagt cactttctgc tcttggaaat gttatttctg ctttggctga gggtagtaca 3121 tatgttccat atcgagatag taaaatgaca agaatccttc aagattcatt aggtggcaac 3181 tgtagaacca ctattgtaat ttgctgctct ccatcatcat acaatgagtc tgaaacaaaa 3241 tctacactct tatttggcca aagggccaaa acaattaaga acacagtttg tgtcaatgtg 3301 gagttaactg cagaacagtg gaaaaagaag tatgaaaaag aaaaagaaaa aaataagatc 3361 ctgcggaaca ctattcagtg gcttgaaaat gagctcaaca gatggcgtaa tggggagacg 3421 gtgcctattg atgaacagtt tgacaaagag aaagccaact tggaagcttt cacagtggat 3481 aaagatatta ctcttaccaa tgataaacca gcaaccgcaa ttggagttat aggaaatttt 3541 actgatgctg aaagaagaaa gtgtgaagaa gaaattgcta aattatacaa acagcttgat 3601 gacaaggatg aagaaattaa ccagcaaagt caactggtag agaaactgaa gacgcaaatg 3661 ttggatcagg aggagctttt ggcatctacc agaagggatc aagacaatat gcaagctgag 3721 ctgaatcgcc ttcaagcaga aaatgatgcc tctaaagaag aagtgaaaga agttttacag 3781 gccctagaag aacttgctgt caattatgat cagaagtctc aggaagttga agacaaaact 3841 aaggaatatg aattgcttag tgatgaattg aatcagaaat cggcaacttt agcgagtata 3901 gatgctgagc ttcagaaact taaggaaatg accaaccacc agaaaaaacg agcagctgag 3961 atgatggcat ctttactaaa agaccttgca gaaataggaa ttgctgtggg aaataatgat 4021 gtaaagcagc ctgagggaac tggcatgata gatgaagagt tcactgttgc aagactctac 4081 attagcaaaa tgaagtcaga agtaaaaacc atggtgaaac gttgcaagca gttagaaagc 4141 acacaaactg agagcaacaa aaaaatggaa gaaaatgaaa aggagttagc agcatgtcag 4201 cttcgtatct ctcaacatga agccaaaatc aagtcattga ctgaatacct tcaaaatgtg 4261 gaacaaaaga aaagacagtt ggaggaatct gtcgatgccc tcagtgaaga actagtccag 4321 cttcgagcac aagagaaagt ccatgaaatg gaaaaggagc acttaaataa ggttcagact 4381 gcaaatgaag ttaagcaagc tgttgaacag cagatccaga gccatagaga aactcatcaa 4441 aaacagatca gtagtttgag agatgaagta gaagcaaaag caaaacttat tactgatctt 4501 caagaccaaa accagaaaat gatgttagag caggaacgtc taagagtaga acatgagaag 4561 ttgaaagcca cagatcagga aaagagcaga aaactacatg aacttacggt tatgcaagat 4621 agacgagaac aagcaagaca agacttgaag ggtttggaag agacagtggc aaaagaactt 4681 cagactttac acaacctgcg caaactcttt gttcaggacc tggctacaag agttaaaaag 4741 agtgctgaga ttgattctga tgacaccgga ggcagcgctg ctcagaagca aaaaatctcc 4801 tttcttgaaa ataatcttga acagctcact aaagtgcaca aacagttggt acgtgataat 4861 gcagatctcc gctgtgaact tcctaagttg gaaaagcgac ttcgagctac agctgagaga 4921 gtgaaagctt tggaatcagc actgaaagaa gctaaagaaa atgcatctcg tgatcgcaaa 4981 cgctatcagc aagaagtaga tcgcataaag gaagcagtca ggtcaaagaa tatggccaga 5041 agagggcatt ctgcacagat tgctaaacct attcgtcccg ggcaacatcc agcagcttct 5101 ccaactcacc caagtgcaat tcgtggagga ggtgcatttg ttcagaacag ccagccagtg 5161 gcagtgcgag gtggaggagg caaacaagtg CTCGAGTACC CATACGACGT ACCTGATTAT 5221 GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT CCCAGACTAC 5281 GCATAGGGAT CCGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA 5341 GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC 5401 CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT 5461 TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG 5521 CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG 5581 CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC 5641 CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG 5701 ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT 5761 TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA 5821 CAGATCCAAG CTGTGACCGG CGCCTACGCT AGGCCACCAT GGCCAAGCCT TTGTCTCAAG 5881 AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG 5941 ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG 6001 TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG 6061 CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA 6121 GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG 6181 TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT 6241 ATGTGTGGGA GGGCTAAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 6301 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 6361 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 6421 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 6481 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 6541 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 6601 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT 6661 CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 6721 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 6781 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 6841 CCGCCTCCCC GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC 6901 CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT 6961 CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 7021 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 7081 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 7141 GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT 7201 GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC 7261 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT 7321 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC 7381 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 7441 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 7501 GAGGCCTAGA CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG 7561 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 7621 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 7681 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 7741 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 7801 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 7861 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 7921 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 7981 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 8041 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 8101 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 8161 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 8221 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 8281 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 8341 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 8401 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 8461 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 8521 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 8581 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 8641 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 8701 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 8761 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 8821 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 8881 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 8941 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 9001 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 9061 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 9121 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 9181 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 9241 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 9301 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 9361 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 9421 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 9481 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 9541 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 9601 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 9661 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA 9721 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG 9781 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 9841 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 9901 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 9961 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC 10021 TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA 10081 AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC 10141 GTTGTAAAAC GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.