pCMV-TRPC5-Neo plasmid

pCMV-TRPC5-Neo plasmid

PVT88655

pCMV-TRPC5-Neo plasmid Information

Plasmid name: pCMV-TRPC5(human)-Neo
Alias: NM_012471.3;PPP1R159; TRP5
Gene lenght: 2922bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TRPC5-Neo plasmid Description

pCMV-TRPC5-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TRPC5-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gcccaactgt actacaaaaa ggtcaactac tcaccgtaca gagaccgcat 961 ccccctgcaa attgtgaggg ctgagacaga gctctctgca gaggagaagg ccttcctcaa 1021 tgctgtggag aagggggact atgccactgt gaagcaggcc cttcaggagg ctgagatcta 1081 ctataatgtt aacatcaact gcatggaccc cttgggccgg agtgccctgc tcattgccat 1141 tgagaacgag aacctggaga tcatggagct actgctgaac cacagcgtgt atgtgggtga 1201 tgcattgctc tatgccatac gcaaggaagt ggtgggcgct gtggagcttc tgctcagcta 1261 caggcggccc agcggagaga agcaggtccc cactctgatg atggacacgc agttctctga 1321 attcacaccg gacatcactc ccatcatgct ggctgcccac accaacaact acgaaatcat 1381 caaattgctt gtccaaaaac gggtcactat cccacggccc caccagatcc gctgcaactg 1441 tgtggagtgt gtgtctagtt cagaggtaga cagcctgcgc cactctcgct cccgactgaa 1501 catctataag gctctggcaa gcccctcact cattgcctta tcaagtgagg accccatcct 1561 aactgccttc cgtctgggct gggagctcaa ggagctcagc aaggtggaga atgagttcaa 1621 ggccgagtat gaggagctct ctcagcagtg caagctcttt gccaaagacc tgctggacca 1681 agctcggagc tccagggaac tggagatcat cctcaaccat cgagatgacc acagtgaaga 1741 gcttgaccct cagaagtacc atgacctggc caagttgaag gtggcaatca aataccacca 1801 gaaagagttt gttgctcagc ccaactgcca acagttgctt gccaccctgt ggtatgatgg 1861 cttccctgga tggcggcgga aacactgggt agtcaagctt ctaacctgca tgaccattgg 1921 gttcctgttt cccatgctgt ctatagccta cctgatctca cccaggagca accttgggct 1981 gttcatcaag aaacccttta tcaagtttat ctgccacaca gcatcctatt tgaccttcct 2041 ctttatgctt ctcctggctt ctcagcacat tgtcaggaca gaccttcatg tacaggggcc 2101 tcccccaact gtcgtggaat ggatgatatt gccttgggtt ctaggtttca tttggggtga 2161 gattaaggaa atgtgggatg gtggatttac tgaatacatc catgactggt ggaacctgat 2221 ggattttgca atgaactccc tctacctggc aactatttcc ctgaagattg tggcctatgt 2281 caagtataat ggttctcgtc caagggagga atgggaaatg tggcacccga ctctgattgc 2341 ggaagcactc ttcgcaatat ccaacatttt aagttcgttg cgtctcatat ccctgttcac 2401 agccaactcc cacttaggac ctctgcagat ctctttggga cgcatgctgc ttgatatcct 2461 caaattcctc tttatctact gcctggtact actagctttt gccaatggac tgaaccagct 2521 ttacttctat tatgaaacca gagctatcga tgagcctaac aactgcaagg ggatccgatg 2581 tgagaaacag aacaatgcct tctccacgct ctttgagact cttcagtcac tcttctggtc 2641 tgtatttggc cttttaaatc tatatgtcac caatgtgaaa gccagacacg aattcaccga 2701 gtttgtagga gctaccatgt ttggaacata caatgtcatc tccctggtag tgctgctgaa 2761 catgctgatt gctatgatga acaactccta tcagcttatt gccgatcatg ctgatatcga 2821 gtggaagttt gcaaggacga agctctggat gagttacttt gatgaaggtg gcaccttgcc 2881 acctcctttc aacatcatcc ccagccccaa gtcatttcta taccttggta actggttcaa 2941 caacaccttc tgccccaaaa gagaccctga cggtagacgg agaaggcgca acttgagaag 3001 tttcacagaa cgcaatgctg acagcctgat acaaaatcaa cattatcagg aagttatcag 3061 gaatttagtc aaaagatatg tggctgctat gataagaaat tccaaaacac atgagggact 3121 tacagaagaa aattttaagg aattaaagca agacatctcc agctttcggt atgaagtgct 3181 tgacctcttg ggaaatagaa aacatccaag gagcttttcc actagcagca ctgaactgtc 3241 tcagagagac gataataatg atggcagtgg tggggctcgg gccaaatcca agagtgtctc 3301 ttttaattta ggctgcaaga aaaagacttg ccatgggcca cctctcatca gaaccatgcc 3361 aaggtccagt ggtgcccaag gaaagtcaaa agctgagtca tcaagcaaac gctccttcat 3421 gggtccttct ctcaagaaac tgggtctcct attctccaaa tttaatggtc atatgtctga 3481 acccagttca gagccaatgt acacaatttc tgatggaatt gttcagcagc actgtatgtg 3541 gcaggacatc agatattctc agatggagaa agggaaagca gaggcctgtt ctcaaagtga 3601 aattaacctc agtgaggtag aattaggtga agtccagggc gctgctcaga gcagtgaatg 3661 ccctctagcc tgttccagct ctcttcactg tgcatccagc atctgctcct caaattctaa 3721 acttttagac tcctcagagg atgtatttga aacttgggga gaggcttgtg acttgctcat 3781 gcacaaatgg ggtgatggac aggaagaaca agttacaact cgcctctaaC TCGAGTCTAG 3841 AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 3901 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 3961 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 4021 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 4081 TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC 4141 CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 4201 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 4261 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 4321 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 4381 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 4441 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 4501 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 4561 TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 4621 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 4681 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 4741 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 4801 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT 4861 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 4921 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 4981 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 5041 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 5101 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 5161 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 5221 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 5281 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 5341 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 5401 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 5461 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 5521 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 5581 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC 5641 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 5701 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 5761 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 5821 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 5881 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 5941 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 6001 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 6061 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 6121 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 6181 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 6241 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 6301 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 6361 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 6421 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 6481 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 6541 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 6601 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 6661 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 6721 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 6781 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 6841 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 6901 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 6961 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 7021 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 7081 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 7141 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 7201 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 7261 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 7321 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 7381 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 7441 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 7501 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 7561 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 7621 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 7681 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 7741 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 7801 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 7861 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 7921 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 7981 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 8041 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 8101 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 8161 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 8221 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT 8281 CGGGAGATCT CCCGATCCCC TATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.