pCAGGS-HA-HAT1 plasmid

pCAGGS-HA-HAT1 plasmid

PVT88621

pCAGGS-HA-HAT1 plasmid Information

Plasmid name: pCAGGS-HA-HAT1(human)
Alias: NM_003642.4;KAT1
Gene lenght: 1257bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAGGS-HA-HAT1 plasmid Description

pCAGGS-HA-HAT1 is a plasmid suitable for protein expression in mammalian cells.

pCAGGS-HA-HAT1 plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGTAC 1741 CCATACGATG TTCCAGATTA CGCTGAATTC ATGGCGGGAT TTGGTGCTAT GGAGAAATTT 1801 TTGGTAGAAT ATAAGAGTGC AGTGGAGAAG AAACTGGCAG AGTACAAATG TAACACCAAC 1861 ACAGCAATTG AACTAAAATT AGTTCGTTTT CCTGAAGATC TTGAAAATGA CATTAGAACT 1921 TTCTTTCCTG AGTATACCCA TCAACTCTTT GGGGATGATG AAACTGCTTT TGGTTACAAG 1981 GGTCTAAAGA TCCTGTTATA CTATATTGCT GGTAGCCTGT CAACAATGTT CCGTGTTGAA 2041 TATGCATCTA AAGTTGATGA GAACTTTGAC TGTGTAGAGG CAGATGATGT TGAGGGCAAA 2101 ATTAGACAAA TCATTCCACC TGGATTTTGC ACAAACACGA ATGATTTCCT TTCTTTACTG 2161 GAAAAGGAAG TTGATTTCAA GCCATTCGGA ACCTTACTTC ATACCTACTC AGTTCTCAGT 2221 CCAACAGGAG GAGAAAACTT TACCTTTCAG ATATATAAGG CTGACATGAC ATGTAGAGGC 2281 TTTCGAGAAT ATCATGAAAG GCTTCAGACC TTTTTGATGT GGTTTATTGA AACTGCTAGC 2341 TTTATTGACG TGGATGATGA AAGATGGCAC TACTTTCTAG TATTTGAGAA GTATAATAAG 2401 GATGGAGCTA CGCTCTTTGC GACCGTAGGC TACATGACAG TCTATAATTA CTATGTGTAC 2461 CCAGACAAAA CCCGGCCACG TGTAAGTCAG ATGCTGATTT TGACTCCATT TCAAGGTCAA 2521 GGCCATGGTG CTCAACTTCT TGAAACAGTT CATAGATACT ACACTGAATT TCCTACAGTT 2581 CTTGATATTA CAGCGGAAGA TCCATCCAAA AGCTATGTGA AATTACGAGA CTTTGTGCTT 2641 GTGAAGCTTT GTCAAGATTT GCCCTGTTTT TCCCGGGAAA AATTAATGCA AGGATTCAAT 2701 GAAGATATGG TGATAGAGGC ACAACAGAAG TTCAAAATAA ATAAGCAACA CGCTAGAAGG 2761 GTTTATGAAA TTCTTCGACT ACTGGTAACT GACATGAGTG ATGCCGAACA ATACAGAAGC 2821 TACAGACTGG ATATTAAAAG AAGACTAATT AGCCCATATA AGAAAAAGCA GAGAGATCTT 2881 GCTAAGATGA GAAAATGTCT CAGACCAGAA GAACTGACAA ACCAGATGAA CCAAATAGAA 2941 ATAAGCATGC AACATGAACA GCTGGAAGAG AGTTTTCAGG AACTAGTGGA AGATTACCGG 3001 CGTGTTATTG AACGACTTGC TCAAGAGtaa GGTACCCGGG CATGCCTCGA GCTAGCAGAT 3061 CTTAATTAAT TAAGGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC 3121 TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA 3181 ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA 3241 ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCCATAT GCTGGCTGCC ATGAACAAAG 3301 GTTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC TGCTGTCCAT TCCTTATTCC 3361 ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT TTGTTTTGTG TTATTTTTTT 3421 CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC CAGATTTTTC CTCCTCTCCT 3481 GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGGAG ATCCCTCGAC CTGCAGCCCA 3541 AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT 3601 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC 3661 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 3721 CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 3781 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 3841 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 3901 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT ATTGCAGCTT ATAATGGTTA 3961 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 4021 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT GCATTAATGA 4081 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 4141 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 4201 GTAATACGGT TATCCACAGA ATCAGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 4261 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 4321 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 4381 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 4441 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 4501 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 4561 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 4621 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 4681 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 4741 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 4801 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 4861 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 4921 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 4981 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 5041 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 5101 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 5161 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 5221 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 5281 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 5341 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 5401 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 5461 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 5521 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 5581 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 5641 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 5701 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 5761 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 5821 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 5881 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 5941 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 6001 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.