pLV3-CMV-ETV4-Fluc-Puro plasmi

pLV3-CMV-ETV4-Fluc-Puro plasmid

PVT88508

pLV3-CMV-ETV4-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-ETV4(human)-Fluc-Puro
Alias: NM_001079675.5;E1A-F; E1AF; PEA3; PEAS3
Gene lenght: 1455bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ETV4-Fluc-Puro plasmid Description

pLV3-CMV-ETV4-Fluc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ETV4-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcGCCAC Catggagcgg aggatgaaag ccggatactt ggaccagcaa 2341 gtgccctaca ccttcagcag caaatcgccc ggaaatggga gcttgcgcga agcgctgatc 2401 ggcccgctgg ggaagctcat ggacccgggc tccctgccgc ccctcgactc tgaagatctc 2461 ttccaggatc taagtcactt ccaggagacg tggctcgctg aagctcaggt accagacagt 2521 gatgagcagt ttgttcctga tttccattca gaaaacctag ctttccacag ccccaccacc 2581 aggatcaaga aggagcccca gagtccccgc acagacccgg ccctgtcctg cagcaggaag 2641 ccgccactcc cctaccacca tggcgagcag tgcctttact ccagtgccta tgaccccccc 2701 agacaaatcg ccatcaagtc ccctgcccct ggtgcccttg gacagtcgcc cctacagccc 2761 tttccccggg cagagcaacg gaatttcctg agatcctctg gcacctccca gccccaccct 2821 ggccatgggt acctcgggga acatagctcc gtcttccagc agcccctgga catttgccac 2881 tccttcacat ctcagggagg gggccgggaa cccctcccag ccccctacca acaccagctg 2941 tcggagccct gcccacccta tccccagcag agctttaagc aagaatacca tgatcccctg 3001 tatgaacagg cgggccagcc agccgtggac cagggtgggg tcaatgggca caggtaccca 3061 ggggcggggg tggtgatcaa acaggaacag acggacttcg cctacgactc agatgtcacc 3121 gggtgcgcat caatgtacct ccacacagag ggcttctctg ggccctctcc aggtgacggg 3181 gccatgggct atggctatga gaaacctctg cgaccattcc cagatgatgt ctgcgttgtc 3241 cctgagaaat ttgaaggaga catcaagcag gaaggggtcg gtgcatttcg agaggggccg 3301 ccctaccagc gccggggtgc cctgcagctg tggcaatttc tggtggcctt gctggatgac 3361 ccaacaaatg cccatttcat tgcctggacg ggccggggaa tggagttcaa gctcattgag 3421 cctgaggagg tcgccaggct ctggggcatc cagaagaacc ggccagccat gaattacgac 3481 aagctgagcc gctcgctccg atactattat gagaaaggca tcatgcagaa ggtggctggt 3541 gagcgttacg tgtacaagtt tgtgtgtgag cccgaggccc tcttctcttt ggccttcccg 3601 gacaatcagc gtccagctct caaggctgag tttgaccggc ctgtcagtga ggaggacaca 3661 gtccctttgt cccacttgga tgagagcccc gcctacctcc cagagctggc tggccccgcc 3721 cagccatttg gccccaaggg tggctactct tactaggcgg ccgcaaggat ctgcgatcgc 3781 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 3841 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 3901 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3961 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4021 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4081 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4141 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4201 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4261 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4321 accatggaga gcgacgagag cggcctgatg gaagacgcca aaaacataaa gaaaggcccg 4381 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag 4441 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc 4501 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg 4561 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg 4621 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa 4681 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag 4741 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc 4801 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat 4861 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca 4921 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct 4981 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc 5041 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact 5101 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag 5161 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta 5221 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa 5281 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc 5341 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt 5401 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg 5461 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt 5521 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg 5581 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac 5641 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc 5701 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt 5761 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag 5821 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag 5881 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac 5941 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgaga 6001 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6061 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 6301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7081 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 7141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 7261 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 7321 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 7381 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 7441 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 7501 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7561 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7621 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7681 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7741 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7801 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7861 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7921 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7981 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8041 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 8401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 8461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 8521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 9421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 9481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10141 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 10201 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 10261 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 10321 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 10381 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 10441 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 10501 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10561 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10621 agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.