pLV3-CMV-FXYD5-3×FLAG-CopGFP-P

pLV3-CMV-FXYD5-3×FLAG-CopGFP-Puro plasmid

PVT88400

pLV3-CMV-FXYD5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FXYD5(human)-3×FLAG-CopGFP-Puro
Alias: NM_001320912.2;DYSAD; HSPC113; IWU1; KCT1; OIT2; PRO6241;RIC;
Gene lenght: 582bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FXYD5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FXYD5-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FXYD5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt cgccctctgg tcgcctgtgt 2341 cttctcacca tcgttggcct gattctcccc accagaggac agacgttgaa agataccacg 2401 tccagttctt cagcagactc aactatcatg gacattcagg tcccgacacg agccccagat 2461 gcagtctaca cagaactcca gcccacctct ccaaccccaa cctggcctgc tgatgaaaca 2521 ccacaacccc agacccagac ccagcaactg gaaggaacgg atgggcctct agtgacagat 2581 ccagagacac acaagagcac caaagcagct catcccactg atgacaccac gacgctctct 2641 gagagaccat ccccaagcac agacgtccag acagaccccc agaccctcaa gccatctggt 2701 tttcatgagg atgacccctt cttctatgat gaacacaccc tccggaaacg ggggctgttg 2761 gtcgcagctg tgctgttcat cacaggcatc atcatcctca ccagtgagaa ctggggttgg 2821 gtgggaagga caccaagacc aggaggggga cttatgacgg agggctgggt tctcaaaggt 2881 ccctgggcaa ttttgtttgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 2941 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3001 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3061 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 3121 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3181 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3241 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3301 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3361 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3421 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3481 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3541 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3601 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3661 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3721 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3781 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3841 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 3901 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 3961 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4021 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4081 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4141 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4201 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4261 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4321 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4381 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4441 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4501 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4561 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4621 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 4681 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4741 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4801 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4861 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4921 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 4981 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5041 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5101 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5161 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5221 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5281 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5341 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5401 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5461 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5521 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 5641 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 5701 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5761 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5821 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 5881 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 5941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6061 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 6121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6181 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6241 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7081 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8461 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8521 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8581 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8641 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8701 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8761 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8821 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.