pCMV-Ogfr-3×Myc-Neo plasmid

pCMV-Ogfr-3×Myc-Neo plasmid

PVT88182

pCMV-Ogfr-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Ogfr(mouse)-3×Myc-Neo
Alias: NM_031373.3;2010013E17Rik;
Gene lenght: 1899bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ogfr-3×Myc-Neo plasmid Description

pCMV-Ogfr-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ogfr-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgga cgacccggaa 721 tgcgattcca cctgggagga cgagagcgag gaggacggcg aggatggcca ggcggatgat 781 acgaccgatg aggacacggg cgacgatgac ggcgacgcgg aggaggcacg gccaagcctg 841 ttccagtcta ggatgacaag gtaccgaaac tggcgtgcta tgcaggacat gcaaagatac 901 cggcacaact acccggattt gacagatcaa gactgcaacg gagacatgtg caacctgagc 961 ttctacaaaa atgagatctg cttccagcca aatggctttc tcattgagga cattcttcag 1021 aactggaaag acaactatga cctcctggaa gagaatcact cctacatcca gtggctgttt 1081 cccctgaggg aaccaggagt gaactggcat gccaagcccc tcacactgaa ggaggttgag 1141 gcatttaaaa gctccaagga agtcagagag cgtcttgtcc gggcctatga gctcatgctg 1201 ggcttctatg ggatccaact tgaggaccgg aacacaggcg ccgtatgccg tgcacagaac 1261 ttccagccac gcttccacaa tctcaacagc cacagccaca acaatctgcg tattacacgc 1321 atcctcaagt cgctgggcga gctgggctta gagcactacc aggcaccgct ggtgcgcttc 1381 ttcctggagg agaccctagt acagcacaaa ctgcccagcg ttcgccagag tgccctggac 1441 tacttcctgt ttgccgtgcg ctgccggcac cagcgccggg agctcgtgca ctttgcttgg 1501 gagcacttca agcctcgccg agagtttgtc tgggggcccc gtgacaagct gcggaggttc 1561 aggccccaga ctatatcccg gccactgatg ggactagggc aggcagataa agatgagggc 1621 cccggggacc cctcccaaga ggctggcacc cagggtcgga cctgtggatc tggaagggac 1681 ctgagtgggg acagtggaac agctgaggat ctctcactgt tgagcgcaaa accccaggat 1741 gtgggaacct tggatgggga ccaaaggcat gaggctaagt ccccgagtcc caaagaaagc 1801 aagaagagga agttggaggg gaacaggcag gagcaggtcc caggggagcc agatcctcag 1861 ggtgtctctg aggtagagaa aattgccctc aaccttgagg ggtgtgccct cagccctacc 1921 agccaggagc ccagggaggc tgaacagccc tgtcttgtgg ctagggtggc taatgaggta 1981 agaaaacgga ggaaggtgga ggaaggagct gagggtgatg gagtagccag taacactcaa 2041 gtgcaggcca gtgccctgtc tcctacccct tcagagtgtc ctgagtccca aaaggatggg 2101 aatgggccag aggaccccaa aagccaggtg gggccagaag accccaaaag ccaggtgggg 2161 ccagaggacc ccaaaagcca ggtggggcca gaggacccca aaagccaggt ggggccagag 2221 gacccaaaag gccaggtgga gccagaggac ccaaaaggcc aggtggggcc agaagaccca 2281 aaaggccagg tggggccaga ggacccaaaa ggccaggtgg ggccagagga ccccaaaagc 2341 caggtggggc cagaggaccc caaaagccag gtggagccag aggaccccaa aagccaggtg 2401 gagccagagg accccaaaag ccaggtggag ccagaggacc ccaaaagcca ggtggggcca 2461 gaggaccccc aaagccaggt ggggccagag caagctgcct ctaagagcct gggggaggat 2521 cctgactctg acactacagg aacctcaatg agtgagtcag aggagttggc aaggattgag 2581 gcctctgttg aacccccaaa gcctCTCGAG GAACAAAAAC TAATATCAGA GGAAGATTTG 2641 GAACAGAAAC TAATCAGTGA AGAAGATTTA GAGCAAAAGT TAATTTCCGA GGAGGACTTA 2701 TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 2761 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 2821 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 2881 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 2941 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 3001 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 3061 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 3121 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 3181 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 3241 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG 3301 CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA 3361 AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT 3421 TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC 3481 CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG 3541 GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCAGATCTGC 3601 GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 3661 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 3721 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 3781 GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 3841 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 3901 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 3961 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA 4021 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 4081 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 4141 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 4201 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 4261 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 4321 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 4381 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 4441 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 4501 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 4561 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 4621 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 4681 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 4741 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 4801 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 4861 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 4921 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 4981 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 5041 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 5101 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 5161 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA 5221 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG 5281 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 5341 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 5401 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 5461 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 5521 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 5581 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 5641 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 5701 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 5761 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 5821 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 5881 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 5941 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 6001 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 6061 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 6121 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 6181 CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 6241 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 6301 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 6361 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 6421 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 6481 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 6541 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 6601 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 6661 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 6721 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 6781 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 6841 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 6901 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 6961 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 7021 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 7081 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 7141 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 7201 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 7261 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 7321 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 7381 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 7441 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 7501 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 7561 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 7621 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 7681 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 7741 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTCGAC 7801 TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT 7861 GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC 7921 CGACAATTGC ATGAAGAATC TGCTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.