pLV2-CMV-EGFP-OR2J2-Puro plasm

pLV2-CMV-EGFP-OR2J2-Puro plasmid

PVT87877

pLV2-CMV-EGFP-OR2J2-Puro plasmid Information

Plasmid name: pLV2-CMV-EGFP-OR2J2(human)-Puro
Alias: NM_030905.3;dJ80I19.4; hs6M1-6; OR6-19; OR6-8; OR6.3.8; ORL684
Gene lenght: 939bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLVX-EGFP-Puro
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-EGFP-OR2J2-Puro plasmid Description

pLV2-CMV-EGFP-OR2J2-Puro This plasmid is suitable for overexpression in mammalian cells. The combination of the CMV enhancer and CMV promoter initiates the transcription of the human OR2J2 gene, and the plasmid carries EGFP green fluorescence for visually observing the expression and localization of the target gene. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, allowing for stable integration of the gene into host cells. The plasmid also carries a Puro resistance gene for selecting host cells that express the target gene.

pLV2-CMV-EGFP-OR2J2-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3541 catggacgag ctgtacaagt ccggactcag atctcgagGC GGCGGAGGGA GTGGAGGAGG 3601 GGGAAGCgga ggcggaggga gtggatccat gaatggcaca gaaggcccta acttctacgt 3661 gcccttctcc aatgcgacgg gtgtggtacg cGCCACTAAC TTCTCCCTGT TGAAACAAGC 3721 AGGGGATGTC GAAGAGAATC CCGGGCCAat gatgattaaa aaaaatgcaa gttcggaaga 3781 cttctttatt ctacttggat tttctaattg gcctcagctg gaagtagttc tctttgtggt 3841 tatcttgatc ttctacctga tgacactgac aggaaacctg ttcatcatca tcctgtcata 3901 cgtggactcc catctccaca caccaatgta cttcttcctt tcaaacctct catttctgga 3961 tctctgctac accaccagct ctatccctca gttgctggtg aatctccggg gcccggaaaa 4021 gaccatctcg tatgctggtt gcatggttca actttacttt gttcttgcac tgggaatcac 4081 agagtgtgtc ctactggtgg tgatgtcata tgatcgttat gtagctgtgt gtagaccttt 4141 gcattacact gtcctcatgc accctcgttt ctgccacttg ttggttgcgg cttcttgggt 4201 aattggtttt actatctcag cacttcattc ctcctttact ttctgggtac ccctttgtgg 4261 acatcgccta gtggatcact tcttctgtga agttccagca cttctgcgtt tatcatgtgt 4321 tgacacccat gcaaatgagc tgaccctcat ggtcatgagc tccatttttg ttctcatacc 4381 tctcattctc attctcacta cctatggtgc cattgcccgg gctgtactga gcatgcaatc 4441 aaccactggg cttcagaaag tgtttaggac atgtggagcc catcttatgg ttgtatctct 4501 ctttttcatt ccagtcatgt gcatgtatct ccagccacca tcagaaaatt ctcctgatca 4561 gggcaagttc attgccctct tttatactgt tgtcacaccg agtcttaatc ctctaatcta 4621 cactctcaga aacaagcatg taaaaggggc agcgaagaga ctattggggt gggagtgggg 4681 gaagtgatct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 4741 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 4801 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 4861 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 4921 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 4981 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 5041 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 5101 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 5161 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 5221 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5281 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5341 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5401 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5461 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5521 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5581 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5641 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5701 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5761 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5821 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 5881 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 5941 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 6001 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 6061 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 6121 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 6181 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 6241 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 6301 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 6361 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 6421 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc 6481 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 6541 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat 6601 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga 6661 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac 6721 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta 6781 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac 6841 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac 6901 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc 6961 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg 7021 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg 7081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7261 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7321 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct 7381 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7441 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7501 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 7621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 7681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 7741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 7801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 7861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 7921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 7981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 8041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 8101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 8161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 8221 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 8581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 8641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 8701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 8761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 8821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 8881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 8941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 9001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 9061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 9121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 9181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9541 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 9601 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 9661 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 9721 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 9781 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 9841 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 9901 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.