pLV3-CMV-PANX1-3×FLAG-CopGFP-P

pLV3-CMV-PANX1-3×FLAG-CopGFP-Puro plasmid

PVT87732

pLV3-CMV-PANX1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PANX1(human)-3×FLAG-CopGFP-Puro
Alias: NM_015368.4;MRS1; OOMD7; OZEMA7; PX1; UNQ2529
Gene lenght: 1278bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PANX1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PANX1-3×FLAG-CopGFP-Puro This is a low-copy plasmid suitable for overexpression in mammalian cells. The plasmid contains a CMV strong promoter that can initiate transcription of the human PANX1 gene, promoting the C-terminal fusion expression of the PANX1 gene with a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid also carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, making it easier for stable integration of the gene into host cells. The plasmid includes the CopGFP green fluorescent protein for detecting the expression and localization of the target gene. Additionally, the plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-PANX1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccatcgctca actggccacg 2341 gagtacgtgt tctcggattt cttgctgaag gagcccacgg agcccaagtt caaggggctg 2401 cgactggagc tggctgtgga caagatggtc acgtgcattg cggtggggct gcccctgctg 2461 ctcatctcgc tggccttcgc gcaggagatc tcgattggta cacagataag ctgtttctct 2521 ccaagttctt tctcctggcg tcaggctgcc tttgtggatt catattgctg ggcggctgtt 2581 cagcagaaga actcactgca gagcgagtct ggaaacctcc cactgtggct gcataagttt 2641 ttcccctaca tcctgctgct ctttgcgatc ctcctgtacc tgcccccgct gttctggcgt 2701 ttcgcagctg ctcctcatat ttgctcagac ttgaagttta tcatggaaga acttgacaaa 2761 gtttacaacc gtgcaattaa ggctgcaaag agtgcgcgtg accttgacat gagagatgga 2821 gcctgctcag ttccaggtgt taccgagaac ttagggcaaa gtttgtggga ggtatctgaa 2881 agccacttca agtacccaat tgtggagcag tacttgaaga caaagaaaaa ttctaataat 2941 ttaatcatca agtacattag ctgccgcctg ctgacactca tcattatact gttagcgtgt 3001 atctacctgg gctattactt cagcctctcc tcactctcag acgagtttgt gtgcagcatc 3061 aaatcaggga tcctgagaaa cgacagcacc gtgcccgatc agtttcagtg caaactcatt 3121 gccgtgggca tcttccagtt gctcagtgtc attaaccttg tggtttatgt cctgctggct 3181 cccgtggttg tctacacgct gtttgttcca ttccgacaga agacagatgt tctcaaagtg 3241 tacgaaatcc tccccacttt tgatgttctg catttcaaat ctgaagggta caacgatttg 3301 agcctctaca atctcttctt ggaggaaaat ataagtgagg tcaagtcata caagtgtctt 3361 aaggtactgg agaatattaa gagcagtggt caggggatcg acccaatgct actcctgaca 3421 aaccttggca tgatcaagat ggatgttgtt gatggcaaaa ctcccatgtc tgcagagatg 3481 agagaggagc aggggaacca gacggcagag ctccaaggta tgaacataga cagtgaaact 3541 aaagcaaata atggagagaa gaatgcccga cagagacttc tggattcttc ttgcggatcc 3601 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3661 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3721 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3781 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3841 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 3901 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 3961 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4021 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4081 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4141 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4201 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4261 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4321 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4381 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4441 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4501 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4561 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4621 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4681 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4741 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4801 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4861 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4921 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 4981 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5041 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5101 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5161 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5221 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5281 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5341 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5401 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5461 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5521 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5581 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5641 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5701 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5761 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5821 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 5881 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 5941 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6001 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6061 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6121 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6181 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6241 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6301 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6541 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6601 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6661 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6721 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 6781 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6841 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 6901 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 6961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9121 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9181 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9241 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9301 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9361 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9421 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9481 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9541 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.