pCMV-SORBS1-3×FLAG-Neo plasmid

pCMV-SORBS1-3×FLAG-Neo plasmid

PVT87228

pCMV-SORBS1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-SORBS1(human)-3×FLAG-Neo
Alias: NM_001034954.3;CAP; FLAF2; R85FL; SH3D5; SH3P12; SORB1
Gene lenght: 3876bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-MCS-3×FLAG-WPRE-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SORBS1-3×FLAG-Neo plasmid Description

pCMV-SORBS1-3×FLAG-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the downstream human SORBS1 gene, promoting the C-terminal fusion expression of the SORBS1 gene with a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The WPRE protein can enhance the processing and stability of the target gene after transcription, helping to increase the half-life and translation efficiency of the mRNA. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-SORBS1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgag ttctgaatgt gatggtggtt 781 ccaaagctgt gatgaatggc ttggcacctg gcagcaatgg gcaagacaaa gcaactgccg 841 accctttacg cgcacgctct atttctgctg ttaaaatcat tcctgtgaag acagtgaaaa 901 acgcctcagg cctagttctc cctacagaca tggatcttac aaaaatctgc actgggaagg 961 gagcggtgac tctccgggcc tcgtcttcct acagggaaac cccaagcagt agccctgcga 1021 gccctcagga aacccggcaa cacgaaagca aaccaggtct ggagccagag ccttcttcag 1081 cagatgagtg gaggctttct tccagtgctg atgccaatgg aaatgcccag ccctcttcac 1141 tcgctgccaa gggctacaga agtgtgcatc ccaaccttcc ttctgacaag tcccaggatg 1201 ccacttcctc cagtgcagcc cagccggagg taatagttgt ccctctctac ctggttaata 1261 ctgacagagg gcaagaaggc actgccagac ctccaacacc tctggggcct cttggctgcg 1321 tccccacaat cccagcgact gcctctgccg cctcacctct gaccttcccg actctagatg 1381 atttcattcc ccctcatctg cagaggtggc cccaccacag ccagccagcc cgcgcctctg 1441 gctcctttgc ccccattagc cagacgccac catccttctc accaccacct ccgctggtcc 1501 ctcctgcccc ggaggacctc cgcagagtct cggagcctga cctcacggga gctgtttcga 1561 gtaccgattc cagtcctcta ctaaatgaag tttcttcttc ccttattgga actgattccc 1621 aagcctttcc atcagttagc aagccttcat ccgcctatcc ctccacaacg attgtcaatc 1681 ctactattgt gctcttgcaa cacaatcgag aacagcaaaa acgactcagt agcctttcag 1741 atcctgtctc agaaagaaga gtgggagagc aggactcagc accaacccag gaaaaaccca 1801 cctcacctgg caaggctatt gaaaaaagag caaaggatga cagtaggcgg gtggtgaaga 1861 gcactcagga cttaagcgat gtttccatgg atgaagtggg catcccactc cggaacactg 1921 agagatcaaa agactggtac aagactatgt ttaaacagat ccacaaactg aacagagaca 1981 ctcctgaaga aaacccttat ttccctacgt acaaattccc tgaacttcct gaaatccagc 2041 aaacttccga agaggacaat ccttacactc ccacctacca gtttcctgca tctactccta 2101 gtcctaaatc tgaagatgat gattcagatc tgtactctcc cagatactca ttttctgaag 2161 acacaaaatc tcccctttct gtgcctcgct caaaaagtga gatgagctac attgatggtg 2221 agaaggtagt caagaggtcg gccacactac ccctcccagc ccgctcttcc tcactgaagt 2281 caagctcaga aagaaatgac tgggaacccc cagataagaa agtagacaca agaaaatatc 2341 gtgcagagcc caagagcatt tacgaatatc agcctggcaa gtcttccgtt ctgaccaacg 2401 aaaagatgag tcgggatata agcccagaag agatagattt aaagaatgaa ccttggtata 2461 aattcttttc ggaattggag tttgggaaac cgcctcccaa aaagatatgg gattatactc 2521 ctggagactg ctctatcctt cctagagagg atagaaagac taatctagac aaagatctca 2581 gcctctgcca gacagagtta gaggcagatt tagaaaaaat ggagacgctt aataaagcac 2641 ccagtgcaaa cgtgccacag agctcagcca tcagccctac tccggaaatt tcttcagaga 2701 ctcctggata tatatattct tccaacttcc atgcagtgaa gagggaatca gacggggctc 2761 ctggggatct cactagcttg gagaatgaga gacaaattta taaaagtgtc ttggaaggtg 2821 gtgacatccc tcttcagggc ctgagtgggc tcaagcgacc atccagctct gcttccacta 2881 aagattcaga atcgccaaga cattttatac cagctgatta cttggaatcc acggaagaat 2941 ttattcgaag acgtcatgat gataaagaga aacttttagc ggaccagaga cgacttaaac 3001 gcgagcaaga agaggctgat attgcagctc gacgccacac aggcgtcatt ccgacgcacc 3061 atcagtttat cactaatgag cgctttgggg acctcctcaa tatagacgat actgcaaaaa 3121 ggaaatctgg gtcagagatg agacctgcca gagccaaatt tgactttaaa gctcagacac 3181 taaaggagct tcctctgcag aagggagata ttgtttacat ttataagcaa attgatcaga 3241 actggtatga aggagaacac cacggccggg tgggaatctt cccacgcacc tacatcgagc 3301 ttcttcctcc tgctgagaag gcacagccca aaaagttgac accagtgcag gttttggaat 3361 atggagaagc tattgctaag tttaacttta atggtgatac acaagtagaa atgtccttca 3421 gaaagggtga gaggatcaca ctgctccggc aggtagatga gaactggtac gaagggagga 3481 tcccggggac atcccgacaa ggcatcttcc ccatcaccta cgtggatgtg atcaagcgac 3541 cactggtgaa aaaccctgtg gattacatgg acctgccttt ctcctcctcc ccaagtcgca 3601 gtgccactgc aagcccacag ttttccagtc acagcaagct catcacgcca gccccctcat 3661 ctctgcccca ctcccgccga gccctgtccc ccgagatgca cgctgtcacc tctgagtgga 3721 tctcactgac tgtgggggtc ccaggcaggc gttctctggc cctgacccca cccttgcctc 3781 ctctgccaga ggcttctatc tataacactg accacctcgc cttgtcacca agggccagtc 3841 cctccctgtc tctcagcctc ccccatttga gttggtcaga tcgtcccacc ccacgatcag 3901 tagcttctcc actggcccta ccttccccac acaaaaccta ctccctagca cctacttccc 3961 aggcctccct tcacatgaat ggagacggtg gtgtccacac gccatcttca ggcatccacc 4021 aagatagctt cttgcagctg ccgctgggga gctctgatag tgtcatctcc cagcttagtg 4081 atgcctttag cagccagagc aagaggcagc catggcgcga agagagtgga caatatgaga 4141 ggaaagcaga gaggggggca ggcgaaagag gccctggtgg acccaagatc tctaagaaga 4201 gctgcttgaa gccttcagac gtggtcaggt gcctgagtac tgaacagaga ctctcagatc 4261 tcaacacccc tgaggagagc cggcccggca agcccctggg tagcgctttt ccaggaagtg 4321 aggctgagca gacagagcgg catagaggtg gcgagcaggc ggggaggaaa gctgctcgga 4381 gaggtgggag ccagcaacct caagcccagc agcgaagagt cacccccgac aggagtcaaa 4441 cctcacaaga tttatttagc tatcaagcat tatatagcta tataccacag aatgatgatg 4501 agttggaact ccgcgatgga gatatcgttg atgtcatgga aaaatgtgac gatggatggt 4561 ttgttggtac ttcaagaagg acaaagcagt ttggtacttt tccaggcaac tatgtaaaac 4621 ctttgtatct aGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG 4681 ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA 4741 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 4801 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 4861 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 4921 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 4981 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 5041 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 5101 TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC 5161 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 5221 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 5281 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA 5341 ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT 5401 AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA 5461 CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC 5521 CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC 5581 AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 5641 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 5701 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 5761 CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 5821 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 5881 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 5941 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 6001 GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 6061 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 6121 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 6181 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 6241 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 6301 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 6361 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 6421 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 6481 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 6541 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 6601 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 6661 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 6721 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 6781 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 6841 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 6901 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 6961 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 7021 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 7081 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 7141 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 7201 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 7261 TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 7321 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 7381 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA 7441 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 7501 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 7561 TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT 7621 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 7681 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 7741 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 7801 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 7861 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 7921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 7981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 8041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 8101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 8161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG 8221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 8281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 8341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 8401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 8461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 8521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 8581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 8641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 8701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 8761 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 8821 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 8881 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 8941 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 9001 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 9061 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 9121 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 9181 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 9241 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 9301 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 9361 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 9421 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 9481 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 9541 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 9601 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 9661 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 9721 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA 9781 TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 9841 CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA 9901 GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG G

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.