pLV3-CMV-Sox6-3×FLAG-CopGFP-Pu

pLV3-CMV-Sox6-3×FLAG-CopGFP-Puro plasmid

PVT87154

pLV3-CMV-Sox6-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Sox6(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001277328.2;SOX-LZ
Gene lenght: 2361bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pCDH-CMV-MCS-3×FLAG-EF1a-CopGFP-T2A-Puro-WPRE
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Sox6-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Sox6-3×FLAG-CopGFP-Puro This is a plasmid suitable for overexpression in mammalian cells. The plasmid contains a CMV strong promoter that can initiate the transcription of the downstream mouse Sox6 gene, promoting the C-terminal fusion expression of a 3×FLAG tag for easy detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid also carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-Sox6-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccATGT CTTCCAAGCA AGCCACCTCT CCATTTGCCT GTACAGCGGA 2341 TGGAGAGGAA GCAATGACCC AGGACTTGAC CTCAAGGGAA AAGGAAGAGG GCAGTGATCA 2401 ACACCCGGCC TCCCATCTGC CTCTGCACCC CATAATGCAC AACAAACCTC ACTCTGAGGA 2461 GCTGCCTACA CTTGTCAGTA CCATTCAACA AGATGCTGAC TGGGACAGCG TTCTGTCATC 2521 TCAGCAAAGA ATGGAGTCAG AGAATAATAA GTTATGTTCC CTATATTCCT TCCGAAATAC 2581 GTCTACCTCA CCACATAAGC CTGACGAAGG GAGTCGGGAG CGTGAAATAA TGAACAGTGT 2641 TACTTTTGGA ACCCCGGAAC GCCGCAAAGG GAGCCTTGCC GATGTGGTGG ATACGCTGAA 2701 GCAGAAGAAG CTGGAGGAGA TGACTCGGAC AGAACAAGAG GATTCCTCCT GCATGGAAAA 2761 ACTACTTTCA AAAGATTGGA AGGAAAAAAT GGAAAGACTA AATACGAGTG AACTTCTTGG 2821 AGAAATCAAA GGTACACCTG AGAGCCTTGC AGAGAAAGAA CGGCAACTCT CCACCATGAT 2881 TACCCAGCTG ATCAGCTTGC GGGAGCAGCT CCTGGCAGCC CATGATGAAC AGAAAAAGCT 2941 GGCAGCATCG CAGATCGAGA AACAGCGGCA GCAAATGGAC CTTGCTCGCC AACAGCAAGA 3001 ACAGATCGCA AGACAACAGC AGCAACTTCT ACAGCAGCAG CACAAGATTA ATCTCCTGCA 3061 GCAACAGATC CAGGTTCAGG GGCACATGCC TCCGCTCATG ATCCCAATTT TTCCACATGA 3121 CCAGCGGACC TTGGCAGCAG CTGCTGCTGC CCAACAGGGA TTCCTCTTCC CCCCTGGAAT 3181 AACATACAAG CCAGGTGATA ACTACCCCGT ACAGTTCATT CCGTCAACAA TGGCAGCTGC 3241 TGCTGCTTCT GGACTCAGCC CTTTACAGCT TCAGCAGCTC TATGCCGCTC AGCTGGCCAG 3301 CATGCAGGTG TCACCTGGAG CAAAGATGCC ATCAACTCCA CAGCCACCAA ACTCGGCAGG 3361 GGCAGTCTCA CCTACTGGGA TAAAAAATGA AAAGAGAGGG ACCAGCCCTG TAACTCAAGT 3421 TAAGGATGAA ACAACAGCCC AGCCGCTGAA TCTCTCATCC CGGCCTAAGA CAGCAGAGCC 3481 TGTGAAGTCC CCAACATCTC CCACCCAGAA CCTCTTCCCA GCCAGCAAAA CCAGCCCTGT 3541 CAACCTGCCA AACAAAAGCA GCATCCCCAG CCCCATTGGA GGAAGCCTGG GGAGAGGATC 3601 CTCTCTAGAT ATACTATCCA GTCTCAACTC TCCTGCCCTG TTTGGGGACC AGGACACAGT 3661 GATGAAAGCC ATTCAGGAGG CTCGGAAGAT GCGAGAACAG ATCCAGCGGG AGCAACAACA 3721 GCAGCCACAC GGAGTTGATG GGAAACTGTC CTCCATGAAC AACATGGGGC TGAGCAACTG 3781 CAGGACTGAG AAGGAAAGAA CACGCTTTGA GAACCTGGGT CCCCAATTAA CAGGAAAGTC 3841 AAGTGAAGAT GGAAAACTGG GCCCGGGTGT CATCGATCTC ACTCGGCCAG AGGACGCAGA 3901 AGGAAGCAAA GCAATGAATG GCTCTGCAGC TAAACTACAG CAGTATTATT GTTGGCCAAC 3961 AGGAGGCGCC ACTGTGGCTG AAGCCCGTGT CTACAGGGAT GCCCGCGGCC GTGCCAGCAG 4021 CGAACCCCAC ATCAAGCGAC CAATGAATGC GTTCATGGTT TGGGCAAAGG ACGAAAGGAG 4081 GAAAATTCTT CAGGCCTTCC CTGACATGCA TAACTCCAAC ATTAGCAAAA TCTTAGGATC 4141 TCGCTGGAAA TCCATGTCCA ACCAGGAGAA GCAACCTTAC TATGAAGAAC AGGCCCGGCT 4201 AAGCAAAATC CACCTAGAGA AGTACCCAAA CTACAAGTAC AAACCCCGGC CGAAGCGCAC 4261 ATGCATCGTG GATGGCAAGA AGCTCCGGAT TGGGGAGTAC AAGCAACTGA TGCGCTCGCG 4321 AAGGCAGGAG ATGCGACAGT TCTTCACTGT GGGGCAACAG CCTCAGATGC CCATCACCAC 4381 AGGAACAGGT GTTGTGTATC CTGGTGCTAT TACTATGGCA ACGACCACAC CATCACCTCA 4441 GATGACATCT GACTGCTCTA GCACCTCTGC CAGCCCCGAG CCCAGCCTCC CTGTCATCCA 4501 GAGCACGTAT GGTATGAAGA TGGACGGCGC AAGCCTGGCT GGGAACGACA TGATTAATGG 4561 AGAGGATGAA ATGGAAGCCT ATGACGACTA CGAAGATGAT CCCAAATCAG ACTATAGCAG 4621 CGAGAATGAG GCCCCAGAGC CTGTCAGTGC CAACggatcc GACTACAAAG ACCATGACGG 4681 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTGAg cggccgcGaa 4741 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 4801 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa 4861 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 4921 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 4981 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 5041 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 5101 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 5161 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 5221 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 5281 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt 5341 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca 5401 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca 5461 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca 5521 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca 5581 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg 5641 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct 5701 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata 5761 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct 5821 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg 5881 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca 5941 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt 6001 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca 6061 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca 6121 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 6181 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 6241 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 6301 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 6361 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 6421 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 6481 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 6541 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 6601 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 6661 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg 6721 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 6781 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 6841 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 6901 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 6961 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 7021 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 7081 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 7141 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 7201 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc 7261 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt 7321 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 7381 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 7441 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 7501 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 7561 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 7621 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 7681 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 7741 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 7801 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 7861 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 7921 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 7981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 8041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 8101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 8161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 8221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 8281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 8341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 8401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 8461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 8521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 8581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 8641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 8701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 8761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8821 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 9061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 9121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 9181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 9241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 9301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 9361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 9421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 9481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 9541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 9601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 9661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 9721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 10021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 10081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 10141 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 10201 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 10261 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 10321 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 10381 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 10441 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 10501 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 10561 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.