pECMV-EGFP-Tjp1-m-FLAG plasmid

pECMV-EGFP-Tjp1-m-FLAG plasmid

PVT87143

pECMV-EGFP-Tjp1-m-FLAG plasmid Information

Plasmid name: pECMV-EGFP-Tjp1-m-FLAG
Alias: -
Gene lenght: 5235bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pECMV-Tjp1-m-FLAG
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pECMV-EGFP-Tjp1-m-FLAG plasmid Description

pECMV-EGFP-Tjp1-m-FLAG This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the mouse Tjp1 gene. The plasmid carries an EGFP green fluorescent protein for labeling and tracking the expression of the protein. Additionally, the plasmid carries an m-FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using antibodies.

pECMV-EGFP-Tjp1-m-FLAG plasmid Sequence

1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCgccacc atggtgagca agggcgagga gctgttcacc 1021 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 1081 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1141 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 1201 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 1261 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 1321 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 1381 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 1441 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 1501 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1561 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1621 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1681 actctcggca tggacgagct gtacaagggc ggtggaggca gtggtggagg cggtagcaaG 1741 GTACCGAGGA GATCTGCCGC CGCGATCGCC ATGTCCGCCA GGGCCGCGGC CGCTAAGAGC 1801 ACAGCAATGG AGGAAACAGC TATATGGGAA CAGCACACAG TGACGCTTCA CAGGGCTCCT 1861 GGGTTTGGAT TTGGAATTGC AATCTCTGGT GGAAGAGATA ATCCTCATTT TCAGAGTGGG 1921 GAAACCTCCA TAGTGATTTC TGATGTGTTA AAAGGAGGGC CAGCTGAAGG ACAGCTACAG 1981 GAAAATGACC GAGTTGCAAT GGTTAACGGA GTTTCAATGG ATAACGTTGA ACATGCTTTT 2041 GCTGTTCAGC AGCTAAGGAA GAGTGGGAAA AACGCAAAAA TTACTATCCG AAGGAAGAAG 2101 AAAGTTCAGA TCCCTGTAAG TCACCCAGAT CCTGAGCCGG TGTCTGATAA TGAAGACGAT 2161 AGTTATGACG AAGAAGTGCA TGACCCAAGA GCTGGCCGCG GTGCTTTAGC GAACAGAAGG 2221 AGCGAGAAGA GCTGGGCAAG GGATAGGAGT GCAAGCAGGG AGAGGAGCCT GTCCCCTCGC 2281 TCGGACAGGC GGTCCGTGGC CTCCAGTCAG CCCGCAAAGC CCACCAAGGT CACACTGGTG 2341 AAGTCTCGGA AAAATGAAGA ATATGGTCTT CGATTGGCCA GCCATATATT TGTAAAGGAA 2401 ATTTCACAAG ATAGTTTGGC AGCAAGAGAT GGTAACATTC AAGAAGGGGA TGTTGTCTTG 2461 AAGATAAATG GTACTGTGAC AGAAAATATG TCATTGACAG ATGCAAAAAC ACTGATAGAA 2521 AGGTCTAAAG GCAAGTTAAA AATGGTAGTG CAAAGAGATG AGCGGGCTAC CTTACTGAAC 2581 GTCCCTGACC TTTCGGATAG TATCCATTCT GCTAATGCCT CGGAAAGAGA TGACATTTCA 2641 GAAATTCAGT CACTAGCATC AGACCATTCA GGTCGCTCGC ATGACAGGCC ACCCCGCCGC 2701 AGCCAGTCAC GATCTCCTGA CCAACGTTCA GAGCCCTCCG ATCATTCCAC GCAGTCTCCA 2761 CAGCAGCCCA GCAATGGCAG TCTCCGGAGC AGAGAGGAAG AGCGAATGTC TAAACCTGGG 2821 GCCATCTCAA CTCCTGTAAA ACATGTAGAC GATCATCCAC CCAAAGCAGT GGAAGAAGTT 2881 ACAGTTGAGA AAAATGAGAA GCAGACGCCC ACTCTTCCAG AACCGAAACC TGTGTATGCT 2941 CAAGTTGGAC AACCAGATGT GGATTTACCC GTCAGCCCTT CTGATGGTGC TCTGCCTAAT 3001 TCAGCTCATG AAGACGGGAT ACTTAGGCCC AGCATGAAAC TGGTAAAATT CAGAAAAGGA 3061 GATAGTGTGG GTTTGCGACT AGCTGGTGGA AATGATGTCG GAATATTTGT AGCTGGCGTT 3121 CTAGAAGATA GCCCTGCAGC CAAAGAAGGC TTAGAGGAAG GTGATCAAAT TCTCAGGGTG 3181 AACAATGTAG ATTTCACAAA TATCATAAGG GAAGAGGCCG TCCTTTTCCT CCTTGACCTC 3241 CCTAAAGGTG AAGAAGTGAC CATACTGGCT CAGAAGAAGA AGGACGTTTA TCGCCGCATT 3301 GTAGAATCAG ATGTAGGAGA TTCATTCTAT ATTAGAACGC ATTTTGAATA TGAAAAAGAA 3361 TCTCCTTACG GACTTAGTTT TAACAAAGGA GAGGTGTTCC GGGTCGTGGA TACTTTATAC 3421 AATGGAAAGC TGGGCTCTTG GCTTGCCATT CGAATTGGCA AAAATCATAA GGAGGTAGAA 3481 CGAGGCATCA TCCCTAATAA GAACAGAGCT GAACAGTTAG CCAGTGTACA GTACACACTT 3541 CCAAAGACAG CGGGTGGTGA TCGGGCAGAC TTCTGGAGGT TTCGAGGTCT TCGCAGCTCC 3601 AAGAGAAATC TTCGAAAAAG CAGAGAGGAC TTGTCAGCTC AGCCAGTTCA AACTAAGTTC 3661 CCAGCTTATG AAAGGGTTGT TCTTCGAGAA GCTGGATTCC TAAGACCTGT AACCATCTTT 3721 GGACCAATAG CTGATGTTGC CAGAGAAAAG TTGGCAAGAG AGGAGCCAGA TATCTATCAG 3781 ATTGCAAAAA GTGAACCACG AGATGCTGGG ACTGACCATC GTAGCTCTGG CATCATTCGC 3841 CTTCATACAA TAAAGCAAAT CATAGATCAG GATAAACATG CTTTATTAGA TGTAACGCCA 3901 AATGCGGTTG ATCGTCTTAA TTATGCGCAG TGGTATCCAA TTGTTGTGTT CCTTAACCCT 3961 GACTCTAAGC AAGGTGTAAA AACAATGAGG ATGAGGTTGT GTCCGGAGTC TCGGAAAAGC 4021 GCCAGGAAGC TATATGAACG CTCTCATAAG CTTCGTAAGA ACAATCACCA TCTCTTCACA 4081 ACTACAATTA ACTTAAACTC AATGAATGAT GGTTGGTACG GTGCCCTGAA AGAAGCGATT 4141 CAGCAGCAAC AGAACCAGCT GGTGTGGGTC TCTGAGGGGA AGGCGGATGG TGCTACAAGT 4201 GATGACCTTG ATTTGCATGA CGATCGTCTG TCCTACCTGT CAGCCCCAGG TAGTGAGTAC 4261 TCAATGTATA GCACGGACAG TAGACACACT TCTGACTATG AAGACACAGA TACAGAAGGC 4321 GGGGCCTACA CTGATCAAGA ACTAGATGAA ACTCTTAATG ATGAGGTGGG GACTCCCCCG 4381 GAGTCTGCCA TTACACGGTC CTCTGAGCCT GTAAGAGAGG ATTCCTCTGG AATGCATCAT 4441 GAAAACCAGA CATACCCTCC TTACTCACCA CAAGCGCAGC CACAAGCTAT TCATAGAATA 4501 GACTCCCCTG GACTTAAGCC AGCCTCTCAA CAGAAAGCAG AAGCCTCATC TCCAGTCCCT 4561 TACCTTTCGC CTGAAACAAC CCCAGCATCA TCAGCCTCTG CAGTTAATCA TAATGTCAGT 4621 GTAACTAATG TCAGCCTGGA GGAGCCTGCC CCAGCCCCTC CCACCTCGCA CGCATCACAG 4681 CCTGGTTGTT TAGGAGCACC AAGTGCTGAG GCAGCTCACG TAGTGCTCAG AGGTGAAGGA 4741 CCACCATTGC CGCCGCATGC AGACCCAGCA AAGGTGTACA GGAAGGAGCC GTATTCTGAA 4801 GAAATGATGA GACAAAACCA TATTTTAAAA CAACCAGCTC TTGGTCACCC AGGGCAGAGG 4861 CCAGATAAAG AGCCAAATCT AGCCTATGAA CCCCAACTTC CATATATAGA AAAACAAGCC 4921 AGCAGAGACC TTGAGCAGCC GTCATACAGG TATGAGGTCT CAAGCTACAC AGACCAGTTT 4981 TCTCGGAACT ATGACCATCG CCTACGGTTT GAAGATCGAA TCCCTACCTA TGAAGACCAG 5041 TGGTCATATT ATGATGACAA ACAGCCCTAC CAACCTCGGC CTTTTGAGAA TCAGCATCCC 5101 CGAGACCTGG ACTCCAGACA ACATCCCGAA GAGGCTTCAG AACGAGGTTA TTTCCAGCGT 5161 TTTGAAGAGC CAGCCCCTCT GTCGTACGAC AGTAGAACAC GCTATGAGCA GCTGCCTCGA 5221 ACCTCTACTC TACGACATGA AGAGCAGCCA GCCCCTGCAT ATGAGGTGCA CAACAGGTAC 5281 AGGCCAGAGG CACAGCCCTA TTCTTCAACA GGCCCTAAGT CATCTGAGCC CAAGCAGTAC 5341 TTTGACCAGT ACCCGCGAAG TTATGAGCAA GTTCCACCAC CAGGCTTTAC CTCCAAAACA 5401 GGCCATTACG AGCCTCTCCA TGGTGCTGCA GTTGTCCCTC CTCTGATACC TTCCTCTCAA 5461 CAAAAGCCAG AAGTCCTGCC CTCGGCTACC AAACCACAGC CTCCACCCCC AACCCTAACT 5521 GAGGAGGAGG AGGATCCAGC AATGAAACCA CAGTCTGTGC TCACCAGAGT CAAAATGTTT 5581 GAAAACAAAA GATCTGCGTC TTTGGAGAAC AAGAAAGATG TGAATGACAC TGCCAGCTTT 5641 AAGCCTCCGG AAGTAGCATC TAAACCTCCA GGTGCTTCTC TTGCTGGCCC TAAACCTGTC 5701 CCTCAGAGTC AGTTTAGTGA GCACGACAAA ACGCTCTACA GGCTCCCAGA GCCTCAGAAA 5761 CCTCAAGTGA AGCCACCCGA AGATATTGTT CGATCAAATC ATTACGACCC TGAAGAGGAT 5821 GAAGAATATT ACCGGAAACA GCTCTCCTAC TTTGACCGAA GAAGTTTTGA GAGCAAGCCT 5881 TCTGCACATC TTCCTGCTGG CCATCACTCA GAGCCTGCTA AGCCAGTCCA TTCTCAGAGC 5941 CAGCCCAACT TCTCTAGTTA CTCTTCAAAG GGAAAACCCG AAACTGATGC TGTGGATAGA 6001 TCATTCAGTG AGAAACGTTA TGATCCAGCC CAGGCCACGC CTCCTCCTCC TCCGTTGCCC 6061 TCACAGTACA GCCAGCCAGC TCCACCTCTG TCCAGCTCTT CTCTCCACAT ACATTCCAAG 6121 GGCGCCCAGG GTGAAGGCAA CTCAGTATCA TTGGATTTTC AGAACTCATA TATGTCCAAA 6181 CCAGACCCAC CCCCATCTCA GAGTAAACCA GCAACTTTCA GACCACCAAC TCGAGAAGAC 6241 CCCCCTCAGA CTTTCTATCC GCAGAAAAGT TTCCCAGACA AAGCTCCAGT TAACGGAGCT 6301 GAGCAGACTC AGAAAACCAT CACTCCGGTG TACAATCGAT TCACACCAAA GCCGTACACA 6361 AGTTCTGCCC GGCCATTTGA ACGCAAATTT GAAAGTCCGA AGTTCAACCA TAATCTTCTG 6421 CCAAGTGAAA CTGTACATAA ACCTGAATTG TCTTCAAAAA CTCCCACTTC CCCAAAAACT 6481 CTTATGAAAG CTCATAGTTC AACACAGCCT CCAGAGTTTG ACAGTGGAGT TGAAACTTTC 6541 TCTGTTCACA CAGATAAGCC TAAATATCAA ATGAATAATA TCAGCACCAT GCCTAAAGCT 6601 GTCCCTGTGA GTCCTTCAGC TGTGGAAGAA GATGAAGATG AGGATGGTCA TACTGTAGTG 6661 GCTACAGCTC GTGGCATTTT TAACAGCAAT GGTGGTGTGT TGAGTTCCAT AGAAACCGGT 6721 GTTAGTATAA TTATCCCACA AGGAGCCATT CCTGAAGGAA TTGAGCAAGA AATCTATTTC 6781 AAAGTCTGCA GAGACAATAG CATTCTCCCA CCTTTAGATA AAGAGAAAGG TGAAACTCTG 6841 CTGAGCCCCC TAGTGATGTG TGGGCCCCAT GGCCTCAAGT TCCTGAAGCC CGTGGAGCTA 6901 CGCTTGCCAC ACTGTGACCC TAAAACCTGG CAAAACAAGT GTCTTCCTGG AGATCCGAAT 6961 TACCTTGTTG GAGCCAACTG TGTTTCTGTC CTGATTGACC ACTTTACGCG TACGCGGCCG 7021 CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG 7081 GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC 7141 CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC 7201 AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC 7261 CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC 7321 AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG 7381 GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT 7441 GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG 7501 GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC 7561 TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT 7621 GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA 7681 TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC 7741 CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT 7801 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 7861 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 7921 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 7981 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 8041 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 8101 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 8161 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 8221 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 8281 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 8341 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 8401 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 8461 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 8521 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 8581 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 8641 AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA 8701 GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT 8761 ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC 8821 CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA 8881 TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT 8941 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA 9001 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG 9061 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC 9121 GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC 9181 GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC 9241 TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG 9301 GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC 9361 GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG 9421 CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC 9481 GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG 9541 CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG 9601 ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC 9661 GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC 9721 TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG 9781 CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA 9841 GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT 9901 CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA 9961 AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT 10021 AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG 10081 GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT 10141 GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA 10201 GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT 10261 GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG 10321 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 10381 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG 10441 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 10501 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 10561 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 10621 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 10681 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 10741 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 10801 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.