pLV3-CMV-Axl-8×His-CopGFP-Puro

pLV3-CMV-Axl-8×His-CopGFP-Puro plasmid

PVT87076

pLV3-CMV-Axl-8×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Axl(mouse)-8×His-CopGFP-Puro
Alias: NM_009465.4;Ark; Tyro7; Ufo
Gene lenght: 2664bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-8×His-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Axl-8×His-CopGFP-Puro plasmid Description

pLV3-CMV-Axl-8×His-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid carries a strong CMV promoter that can initiate the transcription of the mouse Axl gene, promoting the C-terminal fusion expression of the Axl gene with a 6×His tag, facilitating the affinity interaction of the fusion protein with nickel ions for protein purification. It can also be detected and identified using anti-His antibodies, aiding in the separation, purification, and detection of the protein. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, allowing for stable integration of the gene into host cells. The plasmid carries a CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-Axl-8×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcagggtccc gctggcctgg 2341 tggttggcgc tgtgctgctg ggggtgtgca gcccataagg acacacagac cgaggctggc 2401 agcccgtttg tggggaaccc agggaatatc acaggtgcca gaggactcac ggggacactt 2461 cggtgtgagc tccaggttca gggggaaccc cctgaggtgg tgtggcttcg agatggacag 2521 atcctagaac tggctgataa cacccagacc caggtgcctc tgggcgaaga ctggcaagat 2581 gaatggaaag ttgtcagtca gctcagaatc tcagccctgc aactttcaga tgcaggggag 2641 taccagtgta tggtgcatct agaaggacgg acctttgtgt ctcagccggg ctttgtaggg 2701 ctggaaggtc tcccgtactt cctggaggag cctgaggaca aagctgtgcc tgccaacacc 2761 cctttcaacc taagctgcca ggcccaggga cccccggaac ccgtgaccct actctggctt 2821 caagatgctg tccccctggc cccagtcaca ggacacagct cccagcacag tctgcaaact 2881 ccaggcctga acaagacatc ttctttctca tgtgaagccc acaatgccaa gggagtcacc 2941 acctcccgca cagccaccat cacagtgctc ccccagaggc ctcaccatct ccacgtggtt 3001 tccagacaac ctacggagct agaggtagct tggacccctg gcctgagtgg catctacccg 3061 ctcacccact gcaacctgca ggccgtgctg tcagacgatg gggtgggtat ctggctggga 3121 aagtcagatc ctcctgaaga ccccctcacc ttgcaagtat cagtgccccc ccaccagctt 3181 cggctggaaa agctccttcc tcacaccccg tatcacatcc ggatatcctg cagcagcagc 3241 cagggcccct caccttggac ccactggctt cctgtggaga ccacagaggg agtgcccttg 3301 ggtccccctg agaacgttag cgccatgcgg aatgggagcc aggtcctcgt gcgttggcag 3361 gagccaaggg tgcccctgca aggcaccctg ttagggtacc ggctggcata tcgaggccag 3421 gacacccccg aggtacttat ggatataggg ctaactcgag aggtgacctt ggaactgcgg 3481 ggggacaggc ctgtggctaa cctgactgtg tctgtgacag cctatacctc ggctggggat 3541 gggccctgga gccttcctgt gcccctagag ccctggcgcc cagggcaagg acagccactc 3601 caccatctgg tgagtgaacc cccacctcgc gccttctcgt ggccttggtg gtatgtactg 3661 ctgggagcac ttgtggctgc cgcctgcgtc ctcatcttgg ccctgttcct tgtccatcgg 3721 aggaagaagg agactcgata tggggaggtg tttgagccaa ccgtggaaag aggtgaactg 3781 gtagtcaggt accgtgtccg aaagtcctac agccggcgga ccactgaagc caccttgaac 3841 agtctgggca tcagtgaaga gctgaaggag aaactacgag acgtcatggt agatcggcat 3901 aaggtggcct tggggaagac cctgggagaa ggagaatttg gcgctgtgat ggaaggtcag 3961 ctcaatcagg atgactccat cctcaaggtc gctgtgaaga ccatgaaaat tgccatctgc 4021 acaagatcag agctggagga tttcctgagt gaagctgtct gcatgaagga atttgaccac 4081 cccaacgtca tgaggctcat tggcgtctgt tttcagggct ctgacagaga gggtttccca 4141 gaacctgtgg tcatcttgcc tttcatgaaa cacggagacc tacacagttt cctcctgtac 4201 tcccggctcg gggaccagcc agtgttcctg cccactcaga tgctagtgaa gttcatggcc 4261 gacattgcca gtggtatgga gtacctgagt accaagagat tcatacatcg ggacctggct 4321 gccaggaact gcatgctgaa tgagaacatg tccgtgtgtg tggcagactt cgggctctcc 4381 aagaagatct acaacgggga ttactaccgc caagggcgca ttgccaagat gccagtcaag 4441 tggattgcta ttgagagtct ggcagatcgg gtctacacca gcaagagcga tgtgtggtcc 4501 ttcggtgtga caatgtggga gatcgccacc cgaggccaaa ctccctatcc aggggtggag 4561 aacagtgaga tttacgacta cctgcgtcaa ggaaatcggc tgaaacagcc tgtggactgt 4621 ctggacggcc tgtatgccct gatgtctcgg tgctgggaac tgaaccctcg agaccggcca 4681 agttttgcgg agctccggga agacttggag aacacactga aggctctgcc ccctgctcag 4741 gagccagatg aaatcctcta tgtcaacatg gatgagggcg gaagccacct tgaaccccgt 4801 ggggctgctg gaggagctga ccccccaacc caacctgatc ctaaggattc ctgtagctgt 4861 ctcactgcag ctgacgtcca ctcagctgga cgctatgtcc tttgtccttc tacagcccca 4921 ggacccactc tgtctgctga cagaggctgc ccagcacctc cagggcagga ggacggagcc 4981 ggatccCACC ATCACCATCA CCATCACCAT tagcggccgc Gaaggatctg cgatcgctcc 5041 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 5101 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 5161 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 5221 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 5281 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 5341 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 5401 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 5461 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 5521 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 5581 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 5641 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 5701 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 5761 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 5821 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 5881 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 5941 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 6001 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 6061 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 6121 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 6181 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 6241 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 6301 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 6361 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 6421 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 6481 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 6541 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 6601 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 6661 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 6721 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 6781 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 6841 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 6901 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 6961 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 7021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 7081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 7141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 7201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 7261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 7321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 7381 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 7441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7501 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc 7561 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 7621 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 7681 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 7741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 7801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 7861 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 7921 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 7981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 8041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 8101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 8161 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 8221 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 8281 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 8341 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 8401 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 8461 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 8521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 8581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 8641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 8701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 8761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 8821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 8881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 8941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 9001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 9061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 9121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 9181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 9241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 9301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 9361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 9421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 9481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 9541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 9601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 9661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 9721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 9781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 9841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 9901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 9961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 10021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 10081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 10141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 10261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 10321 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 10381 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 10441 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 10501 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 10561 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 10621 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 10681 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 10741 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 10801 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 10861 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.